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CBX8 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS3901393

CBX8 is part of the polycomb group of proteins that are important in embryogenesis and human tumorigenesis and regulates a number of genes involved in cell growth and survival. There are at least 5 CBX proteins in mammals. CBX proteins associate with the core… More details

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Testing Data View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
CBX8, Polyclonal Antibody
Full Product Name
Rabbit anti-CBX8 Antibody, Affinity Purified
Product Gene Name
anti-CBX8 antibody
Product Synonym Names
Pc class 3 homolog; RC1; Polycomb 3 homolog; rectachrome 1; hPc3; chromobox protein homolog 8; chromobox homolog 8 (Pc class homolog, Drosophila); polycomb 3; PC3; Chromobox protein homolog 8; chromobox 8
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Purity/Purification
Antigen Affinity Purified
Concentration
1000ug/ml (lot specific)
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Molecular Weight
43,396 Da
3D Structure
ModBase 3D Structure for Q9HC52
Predicted Reactivity
Mouse
Immunogen
Between 339 and C-terminus
Conjugation
Unconjugated

Product Categories/Family for anti-CBX8 antibody

Primary Antibodies
Applications Tested/Suitable for anti-CBX8 antibody
Western Blot (WB), Immunoprecipitation (IP), Chromatin Immunoprecipitation-Seq (ChIP-Seq)
Application Notes for anti-CBX8 antibody
WB: 1:2000-1:10,000
IP: 6ug/mg lysate
ChIP-Seq: 1-2 ug

Chromatin Immunoprecipitation (ChIP) of anti-CBX8 antibody

Localization of CBX8 Binding Sites by ChIP-sequencing. Chromatin from K562 cells was immunoprecipitated with anti-CBX8 antibody MBS3901393 and analyzed by DNA sequencing. The figure illustrates the peak distribution of CBX8 binding within a 4 Mb region of chromosome 1 as detected using anti-CBX8 antibody MBS3901393. ChIP-seq validation performed by Diogenode, Denville, NJ.
Rabbit Anti-CBX8 Polyclonal Antibody – Cat# MBS3901393

Immunoprecipitation (IP) of anti-CBX8 antibody

Detection of human CBX8 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-CBX8 antibody MBS3901393 (lot MBS3901393-2) used for IP at 6 ug per reaction. CBX8 was also immunoprecipitated by rabbit anti-CBX8 antibody BL3751. For blotting immunoprecipitated CBX8, MBS3901393 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Rabbit Anti-CBX8 Polyclonal Antibody, Immunoprecipitation (IP) – Cat# MBS3901393

Western Blot (WB) of anti-CBX8 antibody

Detection of human CBX8 by western blot. Samples: Whole cell lysate (50 ug) from HeLa and HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CBX8 antibody MBS3901393 (lot MBS3901393-2) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Rabbit Anti-CBX8 Polyclonal Antibody, Western Blot (WB) – Cat# MBS3901393

Other Notes

Small volumes of anti-CBX8 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CBX8 purchase

MBS3901393 is an antibody targeting CBX8. Also known as Rabbit anti-CBX8 Antibody, Affinity Purified. Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for CBX8. It may not necessarily be applicable to this product.

NCBI GI #
10190682
NCBI GeneID
57332
NCBI Accession #
NP_065700.1
UniProt Primary Accession #
Q9HC52
UniProt Secondary Accession #
Q96H39; Q9NR07
UniProt Related Accession #
Q9HC52
GenBank Nucleotide #
NM_020649.2
NCBI Official Full Name
chromobox protein homolog 8
NCBI Official Synonym Full Names
chromobox 8
NCBI Official Symbol
CBX8
NCBI Official Synonym Symbols
PC3; RC1
UniProt Protein Name
Chromobox protein homolog 8
UniProt Synonym Protein Names
Polycomb 3 homolog; Pc3; hPc3; Rectachrome 1
Protein Family
Chromobox protein
UniProt Gene Name
CBX8
UniProt Synonym Gene Names
PC3; RC1; Pc3; hPc3
UniProt Entry Name
CBX8_HUMAN
NCBI Protein Information
chromobox protein homolog 8

UniProt Comments for CBX8

CBX8: Component of a Polycomb group (PcG) multiprotein PRC1- like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility.

Protein type: Transcription, coactivator/corepressor

Chromosomal Location of Human Ortholog: 17q25.3

Cellular Component: nucleoplasm; heterochromatin; nuclear chromatin; PcG protein complex; nucleus

Molecular Function: protein binding; single-stranded RNA binding; ubiquitin-protein ligase activity; methylated histone residue binding

Biological Process: transcription, DNA-dependent; histone ubiquitination; negative regulation of transcription from RNA polymerase II promoter
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Research Articles on CBX8

1. Low CBX8 expression was associated with distant metastasis in colorectal cancer.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-CBX8 antibody

 Reference Product  PubMed Publications
 BMI1 antibody  >6 publications with CBX8 and BMI1
 EZH2 antibody  >5 publications with CBX8 and EZH2
 MLLT3 antibody  >2 publications with CBX8 and MLLT3
 PHC2 antibody  >2 publications with CBX8 and PHC2
 RNF2 antibody  >2 publications with CBX8 and RNF2
 SUZ12 antibody  >1 publications with CBX8 and SUZ12
 PCGF2 antibody  >1 publications with CBX8 and PCGF2

Pathways associated with anti-CBX8 antibody

 Products by Pathway  Pathway Diagram
 Cellular Senescence Pathway antibodies  Cellular Senescence Pathway Diagram
 Cellular Responses To Stress Pathway antibodies  Cellular Responses To Stress Pathway Diagram
 Metabolism Of Proteins Pathway antibodies  Metabolism Of Proteins Pathway Diagram
 Oxidative Stress Induced Senescence Pathway antibodies  Oxidative Stress Induced Senescence Pathway Diagram
 Post-translational Protein Modification Pathway antibodies  Post-translational Protein Modification Pathway Diagram
 SUMO E3 Ligases SUMOylate Target Proteins Pathway antibodies  SUMO E3 Ligases SUMOylate Target Proteins Pathway Diagram
 SUMOylation Pathway antibodies  SUMOylation Pathway Diagram
 SUMOylation Of DNA Damage Response And Repair Proteins Pathway antibodies  SUMOylation Of DNA Damage Response And Repair Proteins Pathway Diagram
 SUMOylation Of RNA Binding Proteins Pathway antibodies  SUMOylation Of RNA Binding Proteins Pathway Diagram

Diseases associated with anti-CBX8 antibody

 Disease Name  Pubmed Publications
 Cell Transformation, Neoplastic Antibodies  >2 publications with CBX8 and Cell Transformation, Neoplastic
 Memory Disorders Antibodies  >1 publications with CBX8 and Memory Disorders
 Brain Diseases Antibodies  >1 publications with CBX8 and Brain Diseases
 Liver Neoplasms Antibodies  >1 publications with CBX8 and Liver Neoplasms
 Death Antibodies  >1 publications with CBX8 and Death
 Disease Models, Animal Antibodies  >1 publications with CBX8 and Disease Models, Animal
 Liver Diseases Antibodies  >1 publications with CBX8 and Liver Diseases

Organs/Tissues associated with anti-CBX8 antibody

 Organ/Tissue Name  Pubmed Publications
 Bone Antibodies  >2 publications with CBX8 and Bone
 Brain Antibodies  >2 publications with CBX8 and Brain
 Lung Antibodies  >2 publications with CBX8 and Lung
 Prostate Antibodies  >1 publications with CBX8 and Prostate
 Bone Marrow Antibodies  >1 publications with CBX8 and Bone Marrow
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