APO AI/AII Reagent – SDS Gel

Name: APO AI/AII Reagent; SDS Gel; MyBioSource
Brand: MyBioSource
SKU: MBS390140
Price: 310 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS390140

MBS390140 is a reagent targeting Apo AI/AII. Also known as Human Apo AI/AII SDS-PAGE Standard. More details

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Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Product Name

Apo AI/AII, Reagent

Full Product Name

Human Apo AI/AII SDS-PAGE Standard

Product Gene Name

Apo AI/AII reagent

Product Synonym Names

Human Apo AI/AII SDS-PAGE Standard, Un-Stained

Form/Format

100 ul (20 applications of 5 ul each). ApoAI/AII Standard is a ready-to-use format - no mixing, heating or reducing required. It is pre-reduced and contains reducing reagents in the loading buffer. The ApoAI/AII resolves into bands with 8.7 and 28 kD.

Concentration

0.20 mg/ml (vortex before apply for gel electrophoresis) (lot specific)

Preparation and Storage

-20o C for short and long-term storage. Aliquot to avoid repeated freezing and thawing.

Molecular Weight

30,062 Da

Chromosome Location

Chromosome: 8; NC_005107.3 (49151163..49152947). Location: 8q23-q24

3D Structure

ModBase 3D Structure for P04639

Source Note

From purified ApoAI and ApoAII; fresh human plasma that has tested negative for Hepatitis C, HIV-I and HIV-II antibodies as well as Hepatitis surface antigens

Buffer

In loading buffer consists of Tris-HCl, MeSH, SDS, glycerol, and bromophenol.

Product Categories/Family for Apo AI/AII reagent

SDS Gel

Applications Tested/Suitable for Apo AI/AII reagent

SDS-PAGE

Application Notes for Apo AI/AII reagent

Apo AI and AII Standard for SDS- PAGE, 0.2 mg/ml, preserved in loading buffer.
Use: The Un-Stained ApoAI/AII Standard allows you to check the result of your gel run and to judge western transfer efficiency. The attached gel was running with 4 ul and 5 ul for lane 1 (ApoAI/AII) and lane 2 (Std Mix2) on an Invitrogen 18% SDS-Gel, respectively.

Other Notes

Small volumes of Apo AI/AII reagent vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for Apo AI/AII. It may not necessarily be applicable to this product.

NCBI GI #

6978515

NCBI GeneID

25081

NCBI Accession #

NP_036870.1

UniProt Primary Accession #

P04639

UniProt Secondary Accession #

O08877; O09054; Q5EBB2

UniProt Related Accession #

P04639

GenBank Nucleotide #

NM_012738.1

NCBI Official Full Name

apolipoprotein A-I preproprotein

NCBI Official Synonym Full Names

apolipoprotein A-I

NCBI Official Symbol

Apoa1

NCBI Official Synonym Symbols

apoA-I

UniProt Protein Name

Apolipoprotein A-I

UniProt Synonym Protein Names

Apolipoprotein A1

UniProt Gene Name

Apoa1

UniProt Synonym Gene Names

Apo-AI; ApoA-I

UniProt Entry Name

APOA1_RAT

NCBI Protein Information

apolipoprotein A-I; apo-AI; apolipoprotein A1; apolipoprotein A-1; preproapolipoprotein A-I

NCBI Summary for Apo AI/AII

major component of high density lipoprotein (HDL) that is involved in intercellular cholesterol transport in astrocytes; cofactor for lecithin cholesterolacyltransferase (LCAT) [RGD, Feb 2006]

UniProt Comments for Apo AI/AII

Function: Participates in the reverse transport of cholesterol from tissues to the liver for excretion by promoting cholesterol efflux from tissues and by acting as a cofactor for the lecithin cholesterol acyltransferase (LCAT). As part of the SPAP complex, activates spermatozoa motility.

Subunit structure: Interacts with APOA1BP and CLU. Component of a sperm activating protein complex (SPAP), consisting of APOA1, an immunoglobulin heavy chain, an immunoglobulin light chain and albumin. Interacts with NDRG1

By similarity.

Subcellular location: Secreted.

Tissue specificity: Major protein of plasma HDL, also found in chylomicrons.

Post-translational modification: Palmitoylated

By similarity.Met-109 is oxidized to methionine sulfoxide

By similarity.Phosphorylation sites are present in the extracellular medium

By similarity.

Sequence similarities: Belongs to the apolipoprotein A1/A4/E family.

Research Articles on Apo AI/AII

1. on neonatal rat ventricular cardiomyocytes, the aldosterone-dependent chronotropic effects of antiapoA-1 IgG involve the nongenomic activation of L-type calcium channels

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with Apo AI/AII reagent

Products by Pathway	Pathway Diagram
ABC-family Proteins Mediated Transport Pathway antibodies	ABC-family Proteins Mediated Transport Pathway Diagram
ABCA Transporters In Lipid Homeostasis Pathway antibodies	ABCA Transporters In Lipid Homeostasis Pathway Diagram
African Trypanosomiasis Pathway antibodies	African Trypanosomiasis Pathway Diagram
African Trypanosomiasis Pathway antibodies	African Trypanosomiasis Pathway Diagram
Amyloids Pathway antibodies	Amyloids Pathway Diagram
Binding And Uptake Of Ligands By Scavenger Receptors Pathway antibodies	Binding And Uptake Of Ligands By Scavenger Receptors Pathway Diagram
Chylomicron-mediated Lipid Transport Pathway antibodies	Chylomicron-mediated Lipid Transport Pathway Diagram
Disease Pathway antibodies	Disease Pathway Diagram
Diseases Associated With Visual Transduction Pathway antibodies	Diseases Associated With Visual Transduction Pathway Diagram
Fat Digestion And Absorption Pathway antibodies	Fat Digestion And Absorption Pathway Diagram

Diseases associated with Apo AI/AII reagent

Disease Name	Pubmed Publications
Cardiovascular Diseases Antibodies	>382 publications with Apo AI/AII and Cardiovascular Diseases
Atherosclerosis Antibodies	>232 publications with Apo AI/AII and Atherosclerosis
Dyslipidemias Antibodies	>163 publications with Apo AI/AII and Dyslipidemias
Heart Diseases Antibodies	>150 publications with Apo AI/AII and Heart Diseases
Hyperlipidemias Antibodies	>117 publications with Apo AI/AII and Hyperlipidemias
Insulin Resistance Antibodies	>81 publications with Apo AI/AII and Insulin Resistance
Inflammation Antibodies	>79 publications with Apo AI/AII and Inflammation
Hypertension Antibodies	>66 publications with Apo AI/AII and Hypertension
Hypercholesterolemia Antibodies	>57 publications with Apo AI/AII and Hypercholesterolemia
Hypertriglyceridemia Antibodies	>55 publications with Apo AI/AII and Hypertriglyceridemia

Organs/Tissues associated with Apo AI/AII reagent

Organ/Tissue Name	Pubmed Publications
Liver Antibodies	>141 publications with Apo AI/AII and Liver
Kidney Antibodies	>38 publications with Apo AI/AII and Kidney
Small Intestine Antibodies	>5 publications with Apo AI/AII and Small Intestine
Embryonic Tissue Antibodies	>3 publications with Apo AI/AII and Embryonic Tissue
Placenta Antibodies	>2 publications with Apo AI/AII and Placenta
Spleen Antibodies	>2 publications with Apo AI/AII and Spleen
Uncharacterized Tissue Antibodies	>1 publications with Apo AI/AII and Uncharacterized Tissue