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BCAS2 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS3901421

Breast cancer amplified sequence 2 (BCAS2) was identified as a gene overexpressed and amplified in breast cancer cell lines and primary breast cancer tissues. BCAS2 has also been identified as a component of the splicesome and as a protein that interacts with… More details

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Product Overview

Online Datasheet in PDF
Product Name
BCAS2, Polyclonal Antibody
Full Product Name
Rabbit anti-BCAS2 Antibody, Affinity Purified
Product Gene Name
Product Synonym Names
pre-mRNA-splicing factor SPF27; spliceosome-associated protein SPF 27; spliceosome associated protein, amplified in breast cancer; Snt309; DNA amplified in mammary carcinoma 1 protein; DAM1; Breast carcinoma-amplified sequence 2; breast carcinoma amplified sequence 2; SPF27; Pre-mRNA-splicing factor SPF27; BCAS2, pre-mRNA processing factor
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human, Mouse
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Purity/Purification
Antigen Affinity Purified
Concentration
1000ug/ml (lot specific)
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Molecular Weight
26,131 Da
Chromosome Location
Chromosome: 1; NC_000001.10 (115110178..115124265, complement). Location: 1p13.2
OMIM
605783
3D Structure
ModBase 3D Structure for O75934
Predicted Reactivity
Zebrafish, Orangutan
Immunogen
Between 175 and C-terminus
Conjugation
Unconjugated

Product Categories/Family for anti-BCAS2 antibody

Applications Tested/Suitable for anti-BCAS2 antibody
Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC)
Application Notes for anti-BCAS2 antibody
WB: 1:2000-1:10,000
IP: 6ug/mg lysate
IHC: 1:1000-1:5000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

Immunohistochemistry (IHC) of anti-BCAS2 antibody

Detection of human BCAS2 by immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-BCAS2 (Cat. No. MBS3901421 Lot2) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB
Rabbit Anti-BCAS2 Polyclonal Antibody – Cat# MBS3901421

Immunohistochemistry (IHC) of anti-BCAS2 antibody

Detection of mouse BCAS2 by immunohistochemistry. Sample: FFPE section of mouse plasmacytoma. Antibody: Affinity purified rabbit anti-BCAS2 (Cat. No. MBS3901421 Lot2) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB
Rabbit Anti-BCAS2 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS3901421

Immunoprecipitation (IP) of anti-BCAS2 antibody

Detection of human BCAS2 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from MCF-7 cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-BCAS2 antibody MBS3901421 (lot MBS3901421-2) used for IP at 6 ug per reaction. BCAS2 was also immunoprecipitated by a previous lot of this antibody (lot MBS3901421-1) and rabbit anti-BCAS2 antibody BL4030 For blotting immunoprecipitated BCAS2, MBS3901421 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Rabbit Anti-BCAS2 Polyclonal Antibody, Immunoprecipitation (IP) – Cat# MBS3901421

Western Blot (WB) of anti-BCAS2 antibody

Detection of human and mouse BCAS2 by western blot. Samples: Whole cell lysate from MCF-7 (5, 15 and 50 ug), and HeLa (50ug), and mouse NIH 3T3 (50ug) cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-BCAS2 antibody MBS3901421 (lot MBS3901421-2) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Rabbit Anti-BCAS2 Polyclonal Antibody, Western Blot (WB) – Cat# MBS3901421

Other Notes

Small volumes of anti-BCAS2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for BCAS2 purchase

MBS3901421 is an antibody targeting BCAS2. Also known as Rabbit anti-BCAS2 Antibody, Affinity Purified. Reactive in Human, Mouse. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for BCAS2. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
pre-mRNA-splicing factor SPF27
NCBI Official Synonym Full Names
breast carcinoma amplified sequence 2
NCBI Official Symbol
BCAS2
NCBI Official Synonym Symbols
DAM1; SPF27; Snt309
UniProt Protein Name
Pre-mRNA-splicing factor SPF27
UniProt Synonym Protein Names
Breast carcinoma-amplified sequence 2; DNA amplified in mammary carcinoma 1 protein; Spliceosome-associated protein SPF 27
UniProt Gene Name
BCAS2
UniProt Synonym Gene Names
DAM1
UniProt Entry Name
SPF27_HUMAN
NCBI Protein Information
pre-mRNA-splicing factor SPF27; breast carcinoma-amplified sequence 2; spliceosome-associated protein SPF 27; DNA amplified in mammary carcinoma 1 protein; spliceosome associated protein, amplified in breast cancer

UniProt Comments for BCAS2

BCAS2: Component of the PRP19-CDC5L complex that forms an integral part of the spliceosome and is required for activating pre-mRNA splicing. May have a scaffolding role in the spliceosome assembly as it contacts all other components of the core complex. Belongs to the SPF27 family.

Protein type: RNA splicing; Spliceosome; Nuclear receptor co-regulator; Nucleolus

Chromosomal Location of Human Ortholog: 1p13.2

Cellular Component: nucleoplasm; spliceosome; DNA replication factor A complex; nucleolus; nucleus; cell junction

Molecular Function: protein binding

Biological Process: RNA splicing, via transesterification reactions; nuclear mRNA splicing, via spliceosome; RNA splicing; gene expression
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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