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USP14 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS3901426

Ubiquitin (Ub) is a highly conserved protein found ubiquitously in eukaryotic organisms. The conjugation of ubiquitin to proteins is an important means to regulate protein activity for many cellular processes by tagging them for degradation. Removal of Ub can… More details

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Product Overview

Online Datasheet in PDF
Product Name
USP14, Polyclonal Antibody
Full Product Name
Rabbit anti-USP14 Antibody, Affinity Purified
Product Gene Name
anti-USP14 antibody
Product Synonym Names
TGT; ubiquitin thioesterase 14; Ubiquitin-specific-processing protease 14; ubiquitin-specific processing protease 14; ubiquitin thiolesterase 14; ubiquitin specific protease 14 (tRNA-guanine transglycosylase); ubiquitin specific peptidase 14 (tRNA-guanine transglycosylase); tRNA-guanine transglycosylase, 60-kD subunit; deubiquitinating enzyme 14; ubiquitin carboxyl-terminal hydrolase 14; Ubiquitin carboxyl-terminal hydrolase 14; ubiquitin specific peptidase 14
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human, Mouse
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Purity/Purification
Antigen Affinity Purified
Concentration
1000ug/ml (lot specific)
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Molecular Weight
56,069 Da
Chromosome Location
Chromosome: 18; NC_000018.9 (158483..213739). Location: 18p11.32
OMIM
607274
3D Structure
ModBase 3D Structure for P54578
Predicted Reactivity
Bovine
Immunogen
Between 444 and C-terminus
Conjugation
Unconjugated

Product Categories/Family for anti-USP14 antibody

Primary Antibodies
Applications Tested/Suitable for anti-USP14 antibody
Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC)
Application Notes for anti-USP14 antibody
WB: 1:2000-1:10,000
IP: 6ug/mg lysate
IHC: 1:500-1:2000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

Immunohistochemistry (IHC) of anti-USP14 antibody

Detection of human USP14 by immunohistochemistry. Samples: FFPE sections of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-USP14 (Cat. No. MBS3901426 Lot1-left and Cat. No. MBS3901425 Lot2-right) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB
Rabbit Anti-USP14 Polyclonal Antibody – Cat# MBS3901426

Immunohistochemistry (IHC) of anti-USP14 antibody

Detection of human USP14 by immunohistochemistry. Samples: FFPE sections of human colon carcinoma. Antibody: Affinity purified rabbit anti-USP14 (Cat. No. MBS3901426 Lot1-left and Cat. No. MBS3901425 Lot2-right) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB.
Rabbit Anti-USP14 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS3901426

Western Blot (WB) of anti-USP14 antibody

Detection of human and mouse USP14 by western blot (h&m) and immunoprecipitation (h). Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), HEK293T (T; 50 ug), and mouse NIH 3T3 (M; 50 ug) cells. Antibodies: Affinity purified rabbit anti-USP14 antibody MBS3901426 used for WB at 0.1 ug/ml (A) and 1.0 ug/ml (B) and used for IP at 3 ug/mg lysate (B). USP14 was also immunoprecipitated by rabbit anti-USP14 antibody MBS3901425, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).
Rabbit Anti-USP14 Polyclonal Antibody, Western Blot (WB) – Cat# MBS3901426

Other Notes

Small volumes of anti-USP14 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for USP14 purchase

MBS3901426 is an antibody targeting USP14. Also known as Rabbit anti-USP14 Antibody, Affinity Purified. Reactive in Human, Mouse. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for USP14. It may not necessarily be applicable to this product.

NCBI GI #
4827050
NCBI GeneID
9097
NCBI Accession #
NP_005142.1
UniProt Primary Accession #
P54578
UniProt Secondary Accession #
Q53XY5
UniProt Related Accession #
P54578
GenBank Nucleotide #
NM_005151.3
NCBI Official Full Name
ubiquitin carboxyl-terminal hydrolase 14 isoform a
NCBI Official Synonym Full Names
ubiquitin specific peptidase 14 (tRNA-guanine transglycosylase)
NCBI Official Symbol
USP14
NCBI Official Synonym Symbols
TGT
UniProt Protein Name
Ubiquitin carboxyl-terminal hydrolase 14
UniProt Synonym Protein Names
Deubiquitinating enzyme 14; Ubiquitin thioesterase 14; Ubiquitin-specific-processing protease 14
UniProt Gene Name
USP14
UniProt Synonym Gene Names
TGT
UniProt Entry Name
UBP14_HUMAN
NCBI Protein Information
ubiquitin carboxyl-terminal hydrolase 14; ubiquitin thioesterase 14; deubiquitinating enzyme 14; ubiquitin thiolesterase 14; ubiquitin-specific processing protease 14; ubiquitin-specific-processing protease 14; tRNA-guanine transglycosylase, 60-kD subunit

UniProt Comments for USP14

USP14: Proteasome-associated deubiquitinase which releases ubiquitin from the proteasome targeted ubiquitinated proteins. Ensures the regeneration of ubiquitin at the proteasome. Is a reversibly associated subunit of the proteasome and a large fraction of proteasome-free protein exists within the cell. Required for the degradation of the chemokine receptor CXCR4 which is critical for CXCL12-induced cell chemotaxis. Serves also as a physiological inhibitor of endoplasmic reticulum-associated degradation (ERAD) under the non-stressed condition by inhibiting the degradation of unfolded endoplasmic reticulum proteins via interaction with ERN1. Indispensable for synaptic development and function at neuromuscular junctions (NMJs). Homodimer (Potential). Associates with the 26S proteasome. Interacts with FANCC, CXCR4 and ERN1. Belongs to the peptidase C19 family. USP14/UBP6 subfamily.

Protein type: Protease; Ubiquitin conjugating system; Ubiquitin-specific protease; EC 3.4.19.12

Chromosomal Location of Human Ortholog: 18p11.32

Cellular Component: proteasome complex; cell surface; cytoplasm; cytoplasmic membrane-bound vesicle; plasma membrane; synapse; nucleus

Molecular Function: protein binding; tRNA guanylyltransferase activity; cysteine-type endopeptidase activity; ubiquitin-specific protease activity; endopeptidase inhibitor activity

Biological Process: proteasomal ubiquitin-dependent protein catabolic process; synaptic transmission; protein deubiquitination; regulation of chemotaxis
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-USP14 antibody

 Reference Product  PubMed Publications
 UCHL5 antibody  >13 publications with USP14 and UCHL5

Diseases associated with anti-USP14 antibody

 Disease Name  Pubmed Publications
 Disease Models, Animal Antibodies  >5 publications with USP14 and Disease Models, Animal
 Brain Diseases Antibodies  >5 publications with USP14 and Brain Diseases
 Carcinoma Antibodies  >4 publications with USP14 and Carcinoma
 Inflammation Antibodies  >3 publications with USP14 and Inflammation
 Liver Diseases Antibodies  >2 publications with USP14 and Liver Diseases
 Ovarian Diseases Antibodies  >2 publications with USP14 and Ovarian Diseases
 Atrophy Antibodies  >2 publications with USP14 and Atrophy
 Liver Neoplasms Antibodies  >2 publications with USP14 and Liver Neoplasms
 Neurobehavioral Manifestations Antibodies  >1 publications with USP14 and Neurobehavioral Manifestations
 Infertility, Male Antibodies  >1 publications with USP14 and Infertility, Male

Organs/Tissues associated with anti-USP14 antibody

 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >14 publications with USP14 and Brain
 Muscle Antibodies  >8 publications with USP14 and Muscle
 Blood Antibodies  >4 publications with USP14 and Blood
 Lung Antibodies  >4 publications with USP14 and Lung
 Bone Antibodies  >3 publications with USP14 and Bone
 Liver Antibodies  >3 publications with USP14 and Liver
 Bone Marrow Antibodies  >2 publications with USP14 and Bone Marrow
 Prostate Antibodies  >2 publications with USP14 and Prostate
 Lymph Antibodies  >2 publications with USP14 and Lymph
 Ovary Antibodies  >2 publications with USP14 and Ovary
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