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G9A/EHMT2 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS3901439

G9A/EHMT2 is a histone H3-Lys-9 methyltransferase. G9A/EHMT2 has been shown to function as a corepressor by associating with transcriptional regulators such as CDP/cut, Blimp-1/PRDI-BF1, and REST/NRSF. G9A/EHMT2 has also been shown to function as a… More details

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Product Overview

Online Datasheet in PDF
Product Name
G9A/EHMT2, Polyclonal Antibody
Full Product Name
Rabbit anti-G9A/EHMT2 Antibody, Affinity Purified
Product Gene Name
anti-G9A/EHMT2 antibody
Product Synonym Gene Name
EHMT2
Product Synonym Names
histone-lysine N-methyltransferase EHMT2; NG36; lysine N-methyltransferase 1C; KMT1C; HLA-B-associated transcript 8; HLA-B associated transcript 8; G9A; histone-lysine N-methyltransferase, H3 lysine-9 specific 3; Protein G9a; H3-K9-HMTase 3; G9A histone methyltransferase; histone H3-K9 methyltransferase 3; euchromatic histone-lysine N-methyltransferase 2; C6orf30; BAT8; ankyrin repeat-containing protein; GAT8; Histone-lysine N-methyltransferase EHMT2; euchromatic histone lysine methyltransferase 2
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Purity/Purification
Antigen Affinity Purified
Concentration
1000ug/ml (lot specific)
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
OMIM
604599
3D Structure
ModBase 3D Structure for Q96KQ7
Immunogen
Between 1160 and C-terminus
Conjugation
Unconjugated

Product Categories/Family for anti-G9A/EHMT2 antibody

Primary Antibodies
Applications Tested/Suitable for anti-G9A/EHMT2 antibody
Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC)
Application Notes for anti-G9A/EHMT2 antibody
WB: 1:2000-1:10,000
IP: 6ug/mg lysate
IHC: 1:1000-1:5000. Epitope retrieval with Tris-EDTA pH 9.0 is recommended for FFPE tissue sections.

Immunohistochemistry (IHC) of anti-G9A/EHMT2 antibody

Detection of human G9A/EHMT2 by immunohistochemistry. Sample: FFPE section of human lung carcinoma. Antibody: Affinity purified rabbit anti-G9A/EHMT2 (Cat. No. MBS3901439 Lot2) used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB
Rabbit Anti-G9A/EHMT2 Polyclonal Antibody – Cat# MBS3901439

Immunoprecipitation (IP) of anti-G9A/EHMT2 antibody

Detection of human G9A/EHMT2 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-G9A/EHMT2 antibody MBS3901439 (lot MBS3901439-2) used for IP at 6 ug per reaction. G9A/EHMT2 was also immunoprecipitated by a previous lot of this antibody (MBS3901439-1) and rabbit anti-G9A/EHMT2 antibody BL4161. For blotting immunoprecipitated G9A/EHMT2, MBS3901439 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Rabbit Anti-G9A/EHMT2 Polyclonal Antibody, Immunoprecipitation (IP) – Cat# MBS3901439

Western Blot (WB) of anti-G9A/EHMT2 antibody

Detection of human G9A/EHMT2 by western blot. Samples: Whole cell lysate (50 ug) from HeLa and HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-G9A/EHMT2 antibody MBS3901439 (lot MBS3901439-2) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Rabbit Anti-G9A/EHMT2 Polyclonal Antibody, Western Blot (WB) – Cat# MBS3901439

Other Notes

Small volumes of anti-G9A/EHMT2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for G9A/EHMT2 purchase

MBS3901439 is an antibody targeting G9A/EHMT2. Also known as Rabbit anti-G9A/EHMT2 Antibody, Affinity Purified. Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for G9A/EHMT2. It may not necessarily be applicable to this product.

NCBI GeneID
10919
NCBI Accession #
NP_006700.2
UniProt Primary Accession #
Q96KQ7
UniProt Protein Name
Histone-lysine N-methyltransferase EHMT2
UniProt Synonym Protein Names
Euchromatic histone-lysine N-methyltransferase 2; HLA-B-associated transcript 8; Histone H3-K9 methyltransferase 3; H3-K9-HMTase 3; Lysine N-methyltransferase 1C; Protein G9a
UniProt Gene Name
EHMT2
UniProt Synonym Gene Names
BAT8; C6orf30; G9A; KMT1C; NG36; H3-K9-HMTase 3
UniProt Entry Name
EHMT2_HUMAN

UniProt Comments for G9A/EHMT2

EHMT2 iso4: Histone methyltransferase. Preferentially methylates 'Lys-9' of histone H3 and 'Lys-27' of histone H3 (in vitro). H3 'Lys-9' methylation represents a specific tag for epigenetic transcriptional repression by recruiting HP1 proteins to methylated histones. Probably targeted to histone H3 by different DNA-binding proteins like E2F6, MGA, MAX and/or DP1. Also methylates histone H1. Belongs to the histone-lysine methyltransferase family. Suvar3-9 subfamily. Part of the E2F6.com-1 complex in G0 phase composed of E2F6, MGA, MAX, TFDP1, CBX3, BAT8, EUHMTASE1, RING1, RNF2, MBLR, L3MBTL2 and YAF2. Interacts with GFI1B, WIZ and EHMT1. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Methyltransferase, protein lysine; Amino Acid Metabolism - lysine degradation; Methyltransferase; EC 2.1.1.43

Chromosomal Location of Human Ortholog: 6p21.31

Cellular Component: nucleoplasm; intermediate filament cytoskeleton; intracellular membrane-bound organelle; nuclear chromatin; nucleus

Molecular Function: protein binding; zinc ion binding; p53 binding; histone lysine N-methyltransferase activity (H3-K9 specific); protein-lysine N-methyltransferase activity; histone-lysine N-methyltransferase activity; histone lysine N-methyltransferase activity (H3-K27 specific)

Biological Process: histone methylation; establishment and/or maintenance of chromatin architecture; peptidyl-lysine di-methylation; DNA methylation; negative regulation of transcription from RNA polymerase II promoter; regulation of DNA replication; histone H3-K9 methylation
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
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