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Irisin (FNDC5) Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS390153

Description: The exercise-inducible myokine Irisin (Boström, et al. 2012) is secreted by cleavage from the transmembrane precursor, FNDC5. Circulating Irisin govern a wide variety of cellular processes relevant to browning, angiogenesis, wound healing, bone… More details

$785 / 0.2 mg +1 FREE 8GB USB
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Testing Data View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
Irisin (FNDC5), Polyclonal Antibody
Full Product Name
Biotinylated Rabbit Anti Irisin Polyclonal Antibody
Product Gene Name
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Mouse, Human
Form/Format
Freeze-dried powder
Concentration
1 mg / ml (lot specific)
Preparation and Storage
Freeze-dried product should be stored refrigerated until opened. After opening, restore to suggested ml volume with distilled water. If it is not completely clear after standing for 1-2 hours at room temperature, centrifuge the product. It is stable for several weeks at 4 degree C as an undiluted liquid. Do not use for more than one day after dilution. For extended storage after reconstitution, we suggest aliquot to avoid repeated freezing and thawing; or the addition of an equal volume of glycerol to make a final glycerol concentration of 50%, followed by storage at -20 degree C. The concentration of protein and buffer salts will decrease to one-half of the original after the addition of glycerol.
Molecular Weight
15,527 Da
OMIM
AK092102 mRNA
Source Note
Polyclonal antibody purified by Irisin Sepharose affinity column and labeled with biotin. Recombinant Irisin is constructed from the coding sequence of mouse (NM 027402.3). rIrisin produce in BL 21 competent cells.
Preservation
In 75 mM Sodium Phosphate, 75 mM NaCl,  0.5 mM EDTA, 0.02% NaN3, pH 7.2 pH 7.2.

Product Categories/Family for anti-FNDC5 antibody

Applications Tested/Suitable for anti-FNDC5 antibody
Immunoblot (IB), ELISA (EIA)
Application Notes for anti-FNDC5 antibody
Dilution for Immunoblot & ELISA range: 1,000 to 4,000.
Centrifuge before use.

Product References and Citations for anti-FNDC5 antibody

Boström, et al. 2012. A PGC1-alpha-dependent myokine that drive brown-fat-like development of white fat and thermogenesis. Nature, 481: 463-468

Other Notes

Small volumes of anti-FNDC5 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for FNDC5 purchase

MBS390153 is an antibody targeting FNDC5. Also known as Biotinylated Rabbit Anti Irisin Polyclonal Antibody. Reactive in Mouse, Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for FNDC5. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Secondary Accession #
UniProt Related Accession #
NCBI Official Full Name
FNDC5 protein
NCBI Official Synonym Full Names
fibronectin type III domain containing 5
NCBI Official Symbol
FNDC5
NCBI Official Synonym Symbols
FRCP2; irisin
UniProt Protein Name
Fibronectin type III domain-containing protein 5
UniProt Synonym Protein Names
Fibronectin type III repeat-containing protein 2
UniProt Gene Name
FNDC5
UniProt Synonym Gene Names
FRCP2
NCBI Protein Information
fibronectin type III domain-containing protein 5

NCBI Summary for FNDC5

This gene encodes a secreted protein that is released from muscle cells during exercise. The encoded protein may participate in the development of brown fat. Translation of the precursor protein initiates at a non-AUG start codon at a position that is conserved as an AUG start codon in other organisms. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2013]
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UniProt Comments for FNDC5

Irisin: Contrary to mouse, may not be involved in the beneficial effects of muscular exercise, nor in the induction of browning of human white adipose tissue.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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