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SMARCB1/SNF5 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS3901607

The SWI/SNF-related, matrix-associated, actin-dependent regulators of chromatin (SMARC), also called BRG1-associated factors (BAFs), have been identified as components of the human SWI/SNF-like chromatin-remodeling protein complexes. SMARCA3 also known as… More details

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Product Overview

Online Datasheet in PDF
Product Name
SMARCB1/SNF5, Polyclonal Antibody
Full Product Name
Rabbit anti-SMARCB1/SNF5 Antibody, Affinity Purified
Product Synonym Gene Name
SMARCB1
Product Synonym Names
SNF5L1; Sfh1p; SNF5; SNF5 homolog; Snr1; sucrose nonfermenting, yeast, homolog-like 1; SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily B member 1; RTPS1; SWNTS1; integrase interactor 1 protein; SWI/SNF-related matrix-associated protein; CSS3; MRD15; BRG1-associated factor 47; RDT; hSNF5; hSNFS; INI1; malignant rhabdoid tumor suppressor; PPP1R144; protein phosphatase 1, regulatory subunit 144; BAF47; SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily B member 1; SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily b, member 1
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human, Mouse
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Purity/Purification
Antigen Affinity Purified
Concentration
1000ug/ml (lot specific)
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Molecular Weight
43,158 Da
OMIM
162091
3D Structure
ModBase 3D Structure for Q12824
Predicted Reactivity
X. laevis, X. tropicalis, Chicken, Bovine
Immunogen
Between 100 and 150
Conjugation
Unconjugated

Product Categories/Family for anti-SMARCB1/SNF5 antibody

Applications Tested/Suitable for anti-SMARCB1/SNF5 antibody
Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC)
Application Notes for anti-SMARCB1/SNF5 antibody
WB: 1:2000-1:10,000
IP: 6ug/mg lysate
IHC: 1:1000 to 1:5000. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.

Immunohistochemistry (IHC) of anti-SMARCB1/SNF5 antibody

Detection of human SMARCB1/SNF5 by immunohistochemistry. Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti SMARCB1/SNF5 (MBS3901607 Lot 3) used at a dilution of 1:2,000 (0.5ug/ml). Detection: DAB
Rabbit Anti-SMARCB1/SNF5 Polyclonal Antibody – Cat# MBS3901607

Immunoprecipitation (IP) of anti-SMARCB1/SNF5 antibody

Detection of human SMARCB1/SNF5 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-SMARCB1/SNF5 antibody (MBS3901607 lot 3) used for IP at 6 ug per reaction. SMARCB1/SNF5 was also immunoprecipitated by a previous lot of this antibody (MBS3901607 lot 2) and a second antibody against a different epitope of SMARCB1/SNF5 (BL708). For blotting immunoprecipitated SMARCB1/SNF5, MBS3901607 was used at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Rabbit Anti-SMARCB1/SNF5 Polyclonal Antibody, Immunoprecipitation (IP) – Cat# MBS3901607

Western Blot (WB) of anti-SMARCB1/SNF5 antibody

Detection of mouse SMARCB1/SNF5 by western blot. Samples: Whole cell lysate (10 ug) from NIH 3T3, CT26, and CH27 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-SMARCB1/SNF5 antibody (MBS3901607 lot 3) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Rabbit Anti-SMARCB1/SNF5 Polyclonal Antibody, Western Blot (WB) – Cat# MBS3901607

Western Blot (WB) of anti-SMARCB1/SNF5 antibody

Detection of human SMARCB1/SNF5 by western blot. Samples: Whole cell lysate (10 ug) from HEK293T, Jurkat, K-562, MCF-7, and HeLa cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-SMARCB1/SNF5 antibody (MBS3901607 lot 3) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.
Rabbit Anti-SMARCB1/SNF5 Polyclonal Antibody, Western Blot (WB) – Cat# MBS3901607

Other Notes

Small volumes of anti-SMARCB1/SNF5 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for SMARCB1/SNF5 purchase

MBS3901607 is an antibody targeting SMARCB1/SNF5. Also known as Rabbit anti-SMARCB1/SNF5 Antibody, Affinity Purified. Reactive in Human, Mouse. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for SMARCB1/SNF5. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily B member 1 isoform a
NCBI Official Synonym Full Names
SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily b, member 1
NCBI Official Symbol
SMARCB1
NCBI Official Synonym Symbols
RDT; INI1; SNF5; Snr1; BAF47; MRD15; RTPS1; Sfh1p; hSNFS; SNF5L1; SWNTS1; PPP1R144
UniProt Protein Name
SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily B member 1
UniProt Synonym Protein Names
BRG1-associated factor 47; BAF47; Integrase interactor 1 protein; SNF5 homolog
UniProt Gene Name
SMARCB1
UniProt Synonym Gene Names
BAF47; INI1; SNF5L1; BAF47; hSNF5
UniProt Entry Name
SNF5_HUMAN
NCBI Protein Information
SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily B member 1; hSNF5; SNF5 homolog; BRG1-associated factor 47; integrase interactor 1 protein; malignant rhabdoid tumor suppressor; SWI/SNF-related matrix-associated protein;

NCBI Summary for SMARCB1/SNF5

The protein encoded by this gene is part of a complex that relieves repressive chromatin structures, allowing the transcriptional machinery to access its targets more effectively. The encoded nuclear protein may also bind to and enhance the DNA joining activity of HIV-1 integrase. This gene has been found to be a tumor suppressor, and mutations in it have been associated with malignant rhabdoid tumors. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
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Precautions

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Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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