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CHD1 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS3901725

CHD1 is a member of the CHD (chromodomain-helicase-DNA-binding) family of proteins that interacts with nucleosomes and plays a role in chromatin remodeling to modulate transcription. The members of the CHD family of proteins possess 3 common structural and… More details

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Product Overview

Online Datasheet in PDF
Product Name
CHD1, Polyclonal Antibody
Full Product Name
Rabbit anti-CHD1 Antibody, Affinity Purified
Product Gene Name
Product Synonym Names
PILBOS; ATP-dependent helicase CHD1; CHD-1; chromodomain-helicase-DNA-binding protein 1; Chromodomain-helicase-DNA-binding protein 1; chromodomain helicase DNA binding protein 1
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human
Form/Format
Liquid. Whole IgG. Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Purity/Purification
Antigen Affinity Purified
Concentration
200ug/ml (lot specific)
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Molecular Weight
196,590 Da
OMIM
602118
3D Structure
ModBase 3D Structure for O14646
Predicted Reactivity
Chicken
Immunogen
Between 1660 and C-terminus
Conjugation
Unconjugated

Product Categories/Family for anti-CHD1 antibody

Applications Tested/Suitable for anti-CHD1 antibody
Western Blot (WB), Immunoprecipitation (IP), ChImmunoprecipitation (IP), Chromatin Immunoprecipitation (ChIP), Chromatin Immunoprecipitation-Seq (ChIP-Seq)
Application Notes for anti-CHD1 antibody
WB: 1:2000-1:10,000
IP: 6ug/mg lysate
ChIP: 1-5 ug
ChIP-chip: 10 ug per
ChIP-Seq: 1 ug

Chromatin Immunoprecipitation (ChIP) of anti-CHD1 antibody

ChIP-chip scatter plot of anti-CHD1 (MBS3901725) enriched DNA binding sites versus input reference DNA. A. 10 ug of MBS3901725 was used to immunoprecipitate chromatin from K-562 cells according to Ren et al (Genes Dev. 2002 16: 245-256). Immunoprecipitated DNA and reference DNA were amplified via ligation-mediated PCR and the products labeled with fluorescent dUTPs. The labeled ChIP and reference DNA were pooled, hybridized to a DNA microarray, and analyzed. Data points below the +3 SD curve (red line) represent significantly enriched binding sites. B. As a control, a similar experiment was performed using normal rabbit IgG. Compared to the anti-CHD1ChIP, normal rabbit IgG showed little enrichment.
Rabbit Anti-CHD1 Polyclonal Antibody – Cat# MBS3901725

Chromatin Immunoprecipitation (ChIP) of anti-CHD1 antibody

Localization of CHD1 Binding Sites by ChIP-sequencing. Chromatin from K562 cells was immunoprecipitated with anti-CHD1 antibody MBS3901725 and analyzed by DNA sequencing. The figure illustrates the peak distribution of CHD1 binding within a 3.5 Mb region of the human X chromosome as detected using anti-CHD1 antibody MBS3901725. ChIP-seq validation performed by Diogenode, Denville, NJ.
Rabbit Anti-CHD1 Polyclonal Antibody, Chromatin Immunoprecipitation (ChIP) – Cat# MBS3901725

Western Blot (WB) of anti-CHD1 antibody

Detection of human CHD1 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and HEK293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-CHD1 antibody MBS3901725 used for WB at 0.04 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. CHD1 was also immunoprecipitated by rabbit anti-CHD1 antibody MBS3901724, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 3 seconds (A) and 10 seconds (B).
Rabbit Anti-CHD1 Polyclonal Antibody, Western Blot (WB) – Cat# MBS3901725

Other Notes

Small volumes of anti-CHD1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CHD1 purchase

MBS3901725 is an antibody targeting CHD1. Also known as Rabbit anti-CHD1 Antibody, Affinity Purified. Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for CHD1. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
chromodomain-helicase-DNA-binding protein 1
NCBI Official Synonym Full Names
chromodomain helicase DNA binding protein 1
NCBI Official Symbol
CHD1
UniProt Protein Name
Chromodomain-helicase-DNA-binding protein 1
UniProt Synonym Protein Names
ATP-dependent helicase CHD1
UniProt Gene Name
CHD1
UniProt Synonym Gene Names
CHD-1
UniProt Entry Name
CHD1_HUMAN
NCBI Protein Information
chromodomain-helicase-DNA-binding protein 1

NCBI Summary for CHD1

The CHD family of proteins is characterized by the presence of chromo (chromatin organization modifier) domains and SNF2-related helicase/ATPase domains. CHD genes alter gene expression possibly by modification of chromatin structure thus altering access of the transcriptional apparatus to its chromosomal DNA template. [provided by RefSeq, Jul 2008]
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UniProt Comments for CHD1

CHD-1: a sequence-selective DNA-binding protein. Contains two chromo (chromatin organization modifier) domains and one SNF2-related helicase and ATPase domains. May play an important role in gene regulation.

Protein type: Helicase; EC 3.6.4.12

Chromosomal Location of Human Ortholog: 5q15-q21

Cellular Component: cytoplasm; nucleus

Molecular Function: ATP-dependent DNA helicase activity; protein binding; DNA binding; methylated histone residue binding; ATP binding

Biological Process: chromatin remodeling; regulation of transcription from RNA polymerase II promoter; transcription, DNA-dependent; DNA duplex unwinding
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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