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PolD3/p66 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS3901748

PolD3/p66 is a subunit of the heterotetrameric DNA polymerase delta that plays a role in DNA replication and repair. The PolD3/p66 subunit has been shown to mediate the interaction between DNA polymerase delta and PCNA (proliferating cell nuclear antigen) to… More details

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Product Overview

Online Datasheet in PDF
Product Name
PolD3/p66, Polyclonal Antibody
Full Product Name
Rabbit anti-PolD3/p66 Antibody, Affinity Purified
Product Gene Name
Product Synonym Gene Name
POLD3
Product Synonym Names
PPP1R128; protein phosphatase 1, regulatory subunit 128; polymerase (DNA-directed), delta 3, accessory subunit; polymerase (DNA) delta 3, accessory subunit; Pol delta C subunit (p66); P66; DNA polymerase delta subunit p68; DNA polymerase delta subunit p66; DNA polymerase delta subunit C; DNA polymerase delta subunit 3; P68; DNA polymerase delta subunit 3; DNA polymerase delta 3, accessory subunit
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Purity/Purification
Antigen Affinity Purified
Concentration
1000ug/ml (lot specific)
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Molecular Weight
51,400 Da
Chromosome Location
Chromosome: 11; NC_000011.9 (74303575..74354105). Location: 11q14
OMIM
611415
3D Structure
ModBase 3D Structure for Q15054
Immunogen
Between 350 and 400
Conjugation
Unconjugated

Product Categories/Family for anti-PolD3/p66 antibody

Applications Tested/Suitable for anti-PolD3/p66 antibody
Immunoprecipitation (IP), Immunohistochemistry (IHC)
Application Notes for anti-PolD3/p66 antibody
IP: 6ug/mg lysate
IHC: 1:500-1:2000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

Immunohistochemistry (IHC) of anti-PolD3/p66 antibody

Detection of human PolD3/p66 by immunohistochemistry. Sample: FFPE section of human small cell lung cancer. Antibody: Affinity purified rabbit anti-PolD3/p66 (Cat. No. MBS3901748 Lot1) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB
Rabbit Anti-PolD3/p66 Polyclonal Antibody – Cat# MBS3901748

Immunoprecipitation (IP) of anti-PolD3/p66 antibody

Detection of human PolD3/p66 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-PolD3/p66 antibody MBS3901748 used for IP at 3 ug/mg lysate. PolD3/p66 was also immunoprecipitated by rabbit anti-PolD3/p66 antibody BL5048, which recognizes an upstream epitope. For blotting immunoprecipitated PolD3/p66, MBS3901749 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Rabbit Anti-PolD3/p66 Polyclonal Antibody, Immunoprecipitation (IP) – Cat# MBS3901748

Other Notes

Small volumes of anti-PolD3/p66 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for PolD3/p66 purchase

MBS3901748 is an antibody targeting PolD3/p66. Also known as Rabbit anti-PolD3/p66 Antibody, Affinity Purified. Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for PolD3/p66. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
DNA polymerase delta subunit 3
NCBI Official Synonym Full Names
polymerase (DNA-directed), delta 3, accessory subunit
NCBI Official Symbol
POLD3
NCBI Official Synonym Symbols
P66; P68
UniProt Protein Name
DNA polymerase delta subunit 3
UniProt Synonym Protein Names
DNA polymerase delta subunit p66
UniProt Gene Name
POLD3
UniProt Synonym Gene Names
KIAA0039
UniProt Entry Name
DPOD3_HUMAN
NCBI Protein Information
DNA polymerase delta subunit 3; Pol delta C subunit (p66); DNA polymerase delta subunit p66

NCBI Summary for PolD3/p66

This gene encodes the 66-kDa subunit of DNA polymerase delta. DNA polymerase delta possesses both polymerase and 3' to 5' exonuclease activity and plays a critical role in DNA replication and repair. The encoded protein plays a role in regulating the activity of DNA polymerase delta through interactions with other subunits and the processivity cofactor proliferating cell nuclear antigen (PCNA). Alternatively spliced transcript variants have been observed for this gene. [provided by RefSeq, Mar 2012]
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UniProt Comments for PolD3/p66

POLD3: Required for optimal DNA polymerase delta activity.

Protein type: Nucleotide Metabolism - pyrimidine; Nucleotide Metabolism - purine; DNA replication

Chromosomal Location of Human Ortholog: 11q14

Cellular Component: nucleoplasm; delta DNA polymerase complex; mitochondrion; nucleus

Molecular Function: protein binding; DNA-directed DNA polymerase activity

Biological Process: telomere maintenance via semi-conservative replication; DNA synthesis during DNA repair; mismatch repair; base-excision repair; nucleotide-excision repair; transcription-coupled nucleotide-excision repair; telomere maintenance via recombination; mitotic cell cycle; nucleotide-excision repair, DNA gap filling; DNA strand elongation during DNA replication; DNA repair; telomere maintenance
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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