Rabbit Anti-SMARCD1/BAF60a Polyclonal Antibody – Cat# MBS3902072

Detection of human SMARCD1/BAF60a by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-SMARCD1/BAF60a antibody MBS3902072 used for IP at 3 ug/mg lysate. SMARCD1/BAF60a was also immunoprecipitated by rabbit anti-SMARCD1/BAF60a antibody BL4525, which recognizes an upstream epitope. For blotting immunoprecipitated SMARCD1/BAF60a, MBS3902073 was used at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

SMARCD1/BAF60a Antibody – Rabbit Polyclonal

Name: SMARCD1/BAF60a Antibody; Polyclonal; Rabbit; Human-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS3902072
Price: 250 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS3902072

The SMARCs (SWI/SNF-related, matrix-associated, actin-dependent regulators of chromatin), and BAFs (BRG1-associated factors), have been identified as components of the mammalian SWI/SNF-like chromatin-remodeling protein complexes. These multi-protein… More details

$250 / 0.01 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

SMARCD1/BAF60a, Polyclonal Antibody

Full Product Name

Rabbit anti-SMARCD1/BAF60a Antibody, Affinity Purified

Product Gene Name

anti-SMARCD1/BAF60a antibody

Product Synonym Gene Name

SMARCD1

Product Synonym Names

Rsc6p; Swp73-like protein; SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily D member 1; SWI/SNF complex 60 kDa subunit A; SWI/SNF complex 60 kDa subunit; mammalian chromatin remodeling complex BRG1-associated factor 60A; CRACD1; chromatin remodeling complex BAF60A subunit; BRG1-associated factor 60A; 60 kDa BRG-1/Brm-associated factor subunit A; BAF60A; SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily D member 1; SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily d, member 1

Clonality

Polyclonal

Host

Rabbit

Species Reactivity

Human

Form/Format

Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide

Purity/Purification

Antigen Affinity Purified

Concentration

1000ug/ml (lot specific)

Preparation and Storage

Store at 2-8 degree C
Shelf Life: 1 year from date of receipt

OMIM

601735

3D Structure

ModBase 3D Structure for Q96GM5

Predicted Reactivity

Mouse, Bovine

Immunogen

Between 50 and 100

Conjugation

Unconjugated

Product Categories/Family for anti-SMARCD1/BAF60a antibody

Primary Antibodies

Applications Tested/Suitable for anti-SMARCD1/BAF60a antibody

Immunoprecipitation (IP)

Application Notes for anti-SMARCD1/BAF60a antibody

IP: 6ug/mg lysate

Other Notes

Small volumes of anti-SMARCD1/BAF60a antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for SMARCD1/BAF60a purchase

MBS3902072 is an antibody targeting SMARCD1/BAF60a. Also known as Rabbit anti-SMARCD1/BAF60a Antibody, Affinity Purified. Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for SMARCD1/BAF60a. It may not necessarily be applicable to this product.

NCBI GeneID

6602

NCBI Accession #

NP_003067.2

UniProt Primary Accession #

Q96GM5

UniProt Protein Name

SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily D member 1

UniProt Synonym Protein Names

60 kDa BRG-1/Brm-associated factor subunit A; BRG1-associated factor 60A; BAF60A; SWI/SNF complex 60 kDa subunit

UniProt Gene Name

SMARCD1

UniProt Synonym Gene Names

BAF60A

UniProt Entry Name

SMRD1_HUMAN

UniProt Comments for SMARCD1/BAF60a

SMARCD1: Involved in chromatin remodeling. Belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and the neuron-specific chromatin remodeling complex (nBAF complex). During neural development a switch from a stem/progenitor to a post-mitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural stem/progenitor cells to post-mitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into neurons, npBAF complexes which contain ACTL6A/BAF53A and PHF10/BAF45A, are exchanged for homologous alternative ACTL6B/BAF53B and DPF1/BAF45B or DPF3/BAF45C subunits in neuron-specific complexes (nBAF). The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural stem cells. The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth. Has a strong influence on the Vitamin D-mediated transcriptional activity from an enhancer Vitamin D receptor element (VDRE). May be a link between mammalian SWI-SNF-like chromatin remodeling complexes and the vitamin D receptor (VDR) heterodimer. Mediates critical interactions between nuclear receptors and the BRG1/SMARCA4 chromatin-remodeling complex for transactivation. Also involved in vitamin D-coupled transcription regulation via its association with the WINAC complex, a chromatin-remodeling complex recruited by vitamin D receptor (VDR), which is required for the ligand- bound VDR-mediated transrepression of the CYP27B1 gene. Belongs to the SMARCD family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Transcription, coactivator/corepressor; Nuclear receptor co-regulator

Chromosomal Location of Human Ortholog: 12q13-q14

Cellular Component: SWI/SNF complex

Molecular Function: protein binding; transcription coactivator activity; protein complex scaffold

Biological Process: regulation of transcription from RNA polymerase II promoter; chromatin remodeling; nervous system development; nucleosome disassembly; chromatin-mediated maintenance of transcription

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

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Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.