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SF3a120/SAP114 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS3902082

SF3a120/SAP114 is a 120 kDa subunit of the heterotrimeric splicing factor 3a (SF3A) protein complex. SF3A along with SF3B and a 12S RNA subunit are components of the 17S U2 small nuclear ribonucleoprotein complex (U2 snRNP) involved in pre-mRNA splicing.… More details

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Product Overview

Online Datasheet in PDF
Product Name
SF3a120/SAP114, Polyclonal Antibody
Full Product Name
Rabbit anti-SF3a120/SAP114 Antibody, Affinity Purified
Product Synonym Gene Name
SF3A1
Product Synonym Names
PRPF21; splicing factor 3a, subunit 1, 120kD; splicing factor 3A subunit 1; splicing factor 3 subunit 1; spliceosome-associated protein 114; SF3A120; SAP 114; PRP21; pre-mRNA splicing factor SF3a (120 kDa subunit); pre-mRNA processing 21; splicing factor 3a, subunit 1, 120kDa; SAP114; Splicing factor 3A subunit 1; splicing factor 3a subunit 1
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human
Form/Format
Liquid. Whole IgG. Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Purity/Purification
Antigen Affinity Purified
Concentration
200ug/ml (lot specific)
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Molecular Weight
81,678 Da
OMIM
605595
3D Structure
ModBase 3D Structure for Q15459
Predicted Reactivity
Mouse, Bovine
Immunogen
Between 743 and C-terminus
Conjugation
Unconjugated
Applications Tested/Suitable for anti-SF3a120/SAP114 antibody
Western Blot (WB), Immunoprecipitation (IP)
Application Notes for anti-SF3a120/SAP114 antibody
WB: 1:2000-1:10,000
IP: 6ug/mg lysate

Western Blot (WB) of anti-SF3a120/SAP114 antibody

Detection of human SF3a120/SAP114 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and HEK293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-SF3a120/SAP114 antibody MBS3902082 used for WB at 0.04 ug/ml (A) and 0.1 ug/ml (B) and used for IP at 3 ug/mg lysate. SF3a120/SAP114 was also immunoprecipitated by rabbit anti-SF3a120/SAP114 antibodies MBS3902079, MBS3902080, and MBS3902081, which recognize upstream epitopes. Detection: Chemiluminescence with exposure times of 10 seconds (A and B).
Rabbit Anti-SF3a120/SAP114 Polyclonal Antibody – Cat# MBS3902082

Other Notes

Small volumes of anti-SF3a120/SAP114 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for SF3a120/SAP114 purchase

MBS3902082 is an antibody targeting SF3a120/SAP114. Also known as Rabbit anti-SF3a120/SAP114 Antibody, Affinity Purified. Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for SF3a120/SAP114. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
splicing factor 3A subunit 1
NCBI Official Synonym Full Names
splicing factor 3a, subunit 1, 120kDa
NCBI Official Symbol
SF3A1
NCBI Official Synonym Symbols
PRP21; PRPF21; SAP114; SF3A120
UniProt Protein Name
Splicing factor 3A subunit 1
UniProt Synonym Protein Names
SF3a120; Spliceosome-associated protein 114; SAP 114
UniProt Gene Name
SF3A1
UniProt Synonym Gene Names
SAP114; SAP 114
UniProt Entry Name
SF3A1_HUMAN
NCBI Protein Information
splicing factor 3A subunit 1; SAP 114; pre-mRNA processing 21; splicing factor 3 subunit 1; spliceosome-associated protein 114; splicing factor 3a, subunit 1, 120kD; pre-mRNA splicing factor SF3a (120 kDa subunit)

NCBI Summary for SF3a120/SAP114

This gene encodes a subunit of the splicing factor 3a protein complex. The splicing factor 3a heterotrimer is a component of the mature U2 small nuclear ribonucleoprotein particle (snRNP). U2 small nuclear ribonucleoproteins play a critical role in spliceosome assembly and pre-mRNA splicing. [provided by RefSeq, Aug 2014]
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UniProt Comments for SF3a120/SAP114

SF3A1: subunit of the splicing factor SF3A required for 'A' complex assembly formed by the stable binding of U2 snRNP to the branchpoint sequence (BPS) in pre-mRNA. Sequence independent binding of SF3A/SF3B complex upstream of the branch site is essential, it may anchor U2 snRNP to the pre-mRNA. May also be involved in the assembly of the 'E' complex.

Protein type: RNA-binding; Nuclear receptor co-regulator; Spliceosome; RNA splicing

Chromosomal Location of Human Ortholog: 22q12.2

Cellular Component: nucleoplasm; spliceosome

Molecular Function: protein binding; RNA binding

Biological Process: nuclear mRNA 3'-splice site recognition; nuclear mRNA splicing, via spliceosome; RNA splicing; gene expression; mRNA processing
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-SF3a120/SAP114 antibody

Diseases associated with anti-SF3a120/SAP114 antibody

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