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NUMB Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS3902168

NUMB plays an essential role in the determination of cell fate and development and appears to be especially important to sensory organogenesis and neurogenesis. NUMB is a notch1-interacting protein that antagonizes the notch1 signaling pathway. In the cell,… More details

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Product Overview

Online Datasheet in PDF
Product Name
NUMB, Polyclonal Antibody
Full Product Name
Rabbit anti-NUMB Antibody, Affinity Purified
Product Gene Name
anti-NUMB antibody
Product Synonym Names
h-Numb; S171; Protein S171; numb homolog; C14orf41; c14_5527; protein numb homolog; Protein numb homolog; NUMB, endocytic adaptor protein
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human
Form/Format
Liquid. Whole IgG. Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Purity/Purification
Antigen Affinity Purified
Concentration
200ug/ml (lot specific)
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Molecular Weight
37,396 Da
OMIM
603728
3D Structure
ModBase 3D Structure for P49757
Predicted Reactivity
Rat
Immunogen
Between 375 and 425
Conjugation
Unconjugated

Product Categories/Family for anti-NUMB antibody

Primary Antibodies
Applications Tested/Suitable for anti-NUMB antibody
Western Blot (WB), Immunoprecipitation (IP)
Application Notes for anti-NUMB antibody
WB: 1:2000-1:10,000
IP: 6ug/mg lysate

Immunoprecipitation (IP) of anti-NUMB antibody

Detection of human NUMB by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-NUMB antibody MBS3902168 (lot MBS3902168-1) used for IP at 3 ug/mg lysate. NUMB was also immunoprecipitated by rabbit anti-NUMB antibody MBS3902169, which recognizes a downstream epitope. For blotting immunoprecipitated NUMB, MBS3902168 was used at 0.1 ug/ml. Detection: Chemiluminescence with exposure time of 30 seconds.
Rabbit Anti-NUMB Polyclonal Antibody – Cat# MBS3902168

Western Blot (WB) of anti-NUMB antibody

Detection of human NUMB by western blot. Samples: Whole cell lysate from HeLa (5, 15 and 50 ug) and HEK293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-NUMB antibody MBS3902168 (lot MBS3902168-1) used for WB at 0.04 ug/ml. Detection: Chemiluminescence with exposure time of 30 seconds.
Rabbit Anti-NUMB Polyclonal Antibody, Western Blot (WB) – Cat# MBS3902168

Other Notes

Small volumes of anti-NUMB antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for NUMB purchase

MBS3902168 is an antibody targeting NUMB. Also known as Rabbit anti-NUMB Antibody, Affinity Purified. Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for NUMB. It may not necessarily be applicable to this product.

NCBI GI #
54144625
NCBI GeneID
8650
NCBI Accession #
NP_001005743.1
UniProt Primary Accession #
P49757
UniProt Secondary Accession #
Q6NUQ7; Q86SY1; Q8WW73; Q9UBG1; Q9UEQ4; B1P2N5; B1P2N6; B1P2N7; B1P2N8; B1P2N9; B4E2B1
UniProt Related Accession #
P49757
GenBank Nucleotide #
NM_001005743.1
NCBI Official Full Name
protein numb homolog isoform 1
NCBI Official Synonym Full Names
NUMB, endocytic adaptor protein
NCBI Official Symbol
NUMB
NCBI Official Synonym Symbols
S171; C14orf41; c14_5527
UniProt Protein Name
Protein numb homolog
UniProt Synonym Protein Names
Protein S171
Protein Family
Protein numb
UniProt Gene Name
NUMB
UniProt Synonym Gene Names
h-Numb
UniProt Entry Name
NUMB_HUMAN
NCBI Protein Information
protein numb homolog

NCBI Summary for NUMB

The protein encoded by this gene plays a role in the determination of cell fates during development. The encoded protein, whose degradation is induced in a proteasome-dependent manner by MDM2, is a membrane-bound protein that has been shown to associate with EPS15, LNX1, and NOTCH1. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2016]
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UniProt Comments for NUMB

NUMB: Plays a role in the process of neurogenesis. Required throughout embryonic neurogenesis to maintain neural progenitor cells, also called radial glial cells (RGCs), by allowing their daughter cells to choose progenitor over neuronal cell fate. Not required for the proliferation of neural progenitor cells before the onset of neurogenesis. Also involved postnatally in the subventricular zone (SVZ) neurogenesis by regulating SVZ neuroblasts survival and ependymal wall integrity. May also mediate local repair of brain ventricular wall damage. Interacts with CDH1 and TFAP2B. Interacts with EPS15, LNX and NOTCH1. May interact with DUOXA1. Interacts with RALBP1 in a complex also containing EPN1 and TFAP2A during interphase and mitosis. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell development/differentiation

Chromosomal Location of Human Ortholog: 14q24.3

Cellular Component: extrinsic to plasma membrane; focal adhesion; clathrin-coated vesicle; apical part of cell; basolateral plasma membrane; integral to plasma membrane; early endosome; plasma membrane; nucleus

Molecular Function: protein binding; cadherin binding; beta-catenin binding; alpha-catenin binding

Biological Process: positive regulation of polarized epithelial cell differentiation; axon guidance; positive regulation of neurogenesis; Notch signaling pathway; negative regulation of Notch signaling pathway; neuroblast division in the subventricular zone; lateral ventricle development; positive regulation of cell migration
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Research Articles on NUMB

1. Methylation of the Numb promoter contributed to hepatocarcinogenesis.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-NUMB antibody

 Products by Pathway  Pathway Diagram
 Activated NOTCH1 Transmits Signal To The Nucleus Pathway antibodies  Activated NOTCH1 Transmits Signal To The Nucleus Pathway Diagram
 Axon Guidance Pathway antibodies  Axon Guidance Pathway Diagram
 Degradation Of GLI1 By The Proteasome Pathway antibodies  Degradation Of GLI1 By The Proteasome Pathway Diagram
 Delta-Notch Signaling Pathway antibodies  Delta-Notch Signaling Pathway Diagram
 Developmental Biology Pathway antibodies  Developmental Biology Pathway Diagram
 Hedgehog 'off' State Pathway antibodies  Hedgehog 'off' State Pathway Diagram
 Hedgehog 'on' State Pathway antibodies  Hedgehog 'on' State Pathway Diagram
 L1CAM Interactions Pathway antibodies  L1CAM Interactions Pathway Diagram
 Notch Signaling Pathway antibodies  Notch Signaling Pathway Diagram
 Notch Signaling Pathway antibodies  Notch Signaling Pathway Diagram

Diseases associated with anti-NUMB antibody

 Disease Name  Pubmed Publications
 Nervous System Diseases Antibodies  >254 publications with NUMB and Nervous System Diseases
 Skin Diseases Antibodies  >51 publications with NUMB and Skin Diseases
 Lymphoma Antibodies  >48 publications with NUMB and Lymphoma
 Carcinoma Antibodies  >46 publications with NUMB and Carcinoma
 Breast Neoplasms Antibodies  >42 publications with NUMB and Breast Neoplasms
 Leukemia Antibodies  >33 publications with NUMB and Leukemia
 Adenocarcinoma Antibodies  >24 publications with NUMB and Adenocarcinoma
 Neoplasms, Experimental Antibodies  >19 publications with NUMB and Neoplasms, Experimental
 Lung Neoplasms Antibodies  >18 publications with NUMB and Lung Neoplasms
 Necrosis Antibodies  >16 publications with NUMB and Necrosis

Organs/Tissues associated with anti-NUMB antibody

 Organ/Tissue Name  Pubmed Publications
 Liver Antibodies  >17 publications with NUMB and Liver
 Kidney Antibodies  >15 publications with NUMB and Kidney
 Lymph Node Antibodies  >8 publications with NUMB and Lymph Node
 Pancreas Antibodies  >7 publications with NUMB and Pancreas
 Spleen Antibodies  >2 publications with NUMB and Spleen
 Ovary Antibodies  >1 publications with NUMB and Ovary
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