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eIF3B/EIF3S9 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS3902208

Eukaryotic initiation factor 3 subunit B (eIF3B) is one of at least 13 non-identical protein subunits of eukaryotic initiation factor 3 (eIF3). eIF3 is the largest eIF (~650 kDa) and functions to facilitate binding of the 40S ribosomal subunit to the 5'-end… More details

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Product Overview

Online Datasheet in PDF
Product Name
eIF3B/EIF3S9, Polyclonal Antibody
Full Product Name
Rabbit anti-eIF3B/EIF3S9 Antibody, Affinity Purified
Product Synonym Gene Name
EIF3B
Product Synonym Names
eukaryotic translation initiation factor 3, subunit 9 (eta, 116kD); prt1 homolog; PRT1; eukaryotic translation initiation factor 3, subunit 9 eta, 116kDa; eukaryotic translation initiation factor 3 subunit B; eukaryotic translation initiation factor 3 subunit 9; EIF3S9; EIF3-P110; EIF3-ETA; eIF3 p110; eIF-3-eta; hPrt1; eIF3b; eIF3 p116; EIF3-P116; Eukaryotic translation initiation factor 3 subunit B; eukaryotic translation initiation factor 3 subunit B
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human, Mouse
Form/Format
Liquid. Whole IgG. Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Purity/Purification
Antigen Affinity Purified
Concentration
200ug/ml (lot specific)
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Molecular Weight
99,029 Da
OMIM
603917
3D Structure
ModBase 3D Structure for P55884
Immunogen
Between 1 and 50
Conjugation
Unconjugated

Product Categories/Family for anti-eIF3B/EIF3S9 antibody

Applications Tested/Suitable for anti-eIF3B/EIF3S9 antibody
Western Blot (WB)
Application Notes for anti-eIF3B/EIF3S9 antibody
WB: 1:2000-1:10,000

Immunoprecipitation (IP) of anti-eIF3B/EIF3S9 antibody

Detection of human and mouse eIF3B/EIF3S9 by western blot of immunoprecipitates. Samples: Whole cell lysate from HeLa (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibody: Affinity purified rabbit anti-eIF3B/EIF3S9 antibody MBS3902208 (lot MBS3902208-1) used for IP at 3 ug/mg lysate. eIF3B/EIF3S9 was immunoprecipitated efficiently by rabbit anti-eIF3B/EIF3S9 antibody MBS3902209, which recognizes a downstream epitope. For blotting immunoprecipitated eIF3B/EIF3S9, MBS3902208 was used at 1.0 ug/ml. Detection: Chemiluminescence with exposure time of 1 second.
Rabbit Anti-eIF3B/EIF3S9 Polyclonal Antibody – Cat# MBS3902208

Western Blot (WB) of anti-eIF3B/EIF3S9 antibody

Detection of human and mouse eIF3B/EIF3S9 by western blot. Samples: Whole cell lysate from HeLa (5, 15 and 50 ug), HEK293T (T; 50 ug), and mouse NIH 3T3 (M; 50 ug) cells. Antibody: Affinity purified rabbit anti-eIF3B/EIF3S9 antibody MBS3902208 (lot MBS3902208-1) used for WB at 0.04 ug/ml. Detection: Chemiluminescence with exposure time of 30 second.
Rabbit Anti-eIF3B/EIF3S9 Polyclonal Antibody, Western Blot (WB) – Cat# MBS3902208

Other Notes

Small volumes of anti-eIF3B/EIF3S9 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for eIF3B/EIF3S9 purchase

MBS3902208 is an antibody targeting eIF3B/EIF3S9. Also known as Rabbit anti-eIF3B/EIF3S9 Antibody, Affinity Purified. Reactive in Human, Mouse. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for eIF3B/EIF3S9. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
eukaryotic translation initiation factor 3 subunit B
NCBI Official Synonym Full Names
eukaryotic translation initiation factor 3, subunit B
NCBI Official Symbol
EIF3B
NCBI Official Synonym Symbols
PRT1; EIF3S9; EIF3-ETA; EIF3-P110; EIF3-P116
UniProt Protein Name
Eukaryotic translation initiation factor 3 subunit B
UniProt Synonym Protein Names
Eukaryotic translation initiation factor 3 subunit 9; Prt1 homolog
; eIF3 p110
UniProt Gene Name
EIF3B
UniProt Synonym Gene Names
eIF3b
UniProt Entry Name
EIF3B_HUMAN
NCBI Protein Information
eukaryotic translation initiation factor 3 subunit B; EIF3B; hPrt1; eIF-3-eta; eIF3 p110; eIF3 p116; prt1 homolog; eukaryotic translation initiation factor 3 subunit 9; eukaryotic translation initiation factor 3, subunit 9 eta, 116kDa

UniProt Comments for eIF3B/EIF3S9

eIF3-eta: eukaryotic translation initiation factor 3 subunit 9. Binds to the 40S ribosome and promotes the binding of methionyl-tRNAi and mRNA. eIF-3 is composed of at least 12 different subunits.

Protein type: Translation initiation; Translation

Chromosomal Location of Human Ortholog: 7p22.3

Cellular Component: eukaryotic translation initiation factor 3 complex; nucleoplasm; cytoplasm; cytosol

Molecular Function: protein binding; translation initiation factor binding; translation initiation factor activity; nucleotide binding; protein complex scaffold

Biological Process: cellular protein metabolic process; translation; translational initiation; gene expression; formation of translation preinitiation complex; regulation of translational initiation
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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