• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

NUP35 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS3902228

The nuclear pore complex (NPC) is a structure that spans the nuclear envelope and provides a conduit between the nucleus and cytoplasm for the nucleocytoplasmic transport of macromolecules. The main components of the NPC are nucleoporins. There are about 30… More details

$250 / 0.01 mg +1 FREE 8GB USB
Product Documentation
PDF
Sample Manual Insert View PDF
PDF
Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
PDF
Material Safety Data Sheet View PDF
PDF
Technical Datasheet View PDF
PDF
Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
NUP35, Polyclonal Antibody
Full Product Name
Rabbit anti-NUP35 Antibody, Affinity Purified
Product Gene Name
anti-NUP35 antibody
Product Synonym Names
NP44; NUP53; nucleoporin NUP53; nucleoporin NUP35; nuclear pore complex protein Nup53; MP-44; MP44; mitotic phosphoprotein 44; 35 kDa nucleoporin; nucleoporin 35kDa; Nucleoporin NUP53; nucleoporin 35
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human, Mouse
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Purity/Purification
Antigen Affinity Purified
Concentration
1000ug/ml (lot specific)
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Molecular Weight
21,142 Da
OMIM
608140
3D Structure
ModBase 3D Structure for Q8NFH5
Predicted Reactivity
Rat
Immunogen
Between 276 and C-terminus
Conjugation
Unconjugated

Product Categories/Family for anti-NUP35 antibody

Primary Antibodies
Applications Tested/Suitable for anti-NUP35 antibody
Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC)
Application Notes for anti-NUP35 antibody
WB: 1:2000-1:10,000
IP: 6ug/mg lysate
IHC: 1:500-1:2000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

Immunohistochemistry (IHC) of anti-NUP35 antibody

Detection of human NUP35 by immunohistochemistry. Sample: FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti-NUP35 (Cat. No. MBS3902228 Lot2) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB
Rabbit Anti-NUP35 Polyclonal Antibody – Cat# MBS3902228

Immunoprecipitation (IP) of anti-NUP35 antibody

Detection of human NUP35 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-NUP35 antibody MBS3902228 (lot MBS3902228-2) used for IP at 6 ug per reaction. NUP35 was also immunoprecipitated by a previous lot of this antibody (lot MBS3902228-1) and rabbit anti-NUP35 antibody BL6800 For blotting immunoprecipitated NUP35, MBS3902228 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Rabbit Anti-NUP35 Polyclonal Antibody, Immunoprecipitation (IP) – Cat# MBS3902228

Western Blot (WB) of anti-NUP35 antibody

Detection of human and mouse NUP35 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-NUP35 antibody MBS3902228 (lot MBS3902228-2) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Rabbit Anti-NUP35 Polyclonal Antibody, Western Blot (WB) – Cat# MBS3902228

Other Notes

Small volumes of anti-NUP35 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for NUP35 purchase

MBS3902228 is an antibody targeting NUP35. Also known as Rabbit anti-NUP35 Antibody, Affinity Purified. Reactive in Human, Mouse. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for NUP35. It may not necessarily be applicable to this product.

NCBI GI #
31982904
NCBI GeneID
129401
NCBI Accession #
NP_612142.2
UniProt Primary Accession #
Q8NFH5
UniProt Secondary Accession #
Q4ZFZ9; Q53S95; Q8TDJ1; B4DP57; B4DYB4
UniProt Related Accession #
Q8NFH5
GenBank Nucleotide #
NM_138285.4
NCBI Official Full Name
nucleoporin NUP53 isoform a
NCBI Official Synonym Full Names
nucleoporin 35kDa
NCBI Official Symbol
NUP35
NCBI Official Synonym Symbols
MP44; NP44; MP-44; NUP53
UniProt Protein Name
Nucleoporin NUP53
UniProt Synonym Protein Names
35 kDa nucleoporin; Mitotic phosphoprotein 44; MP-44; Nuclear pore complex protein Nup53; Nucleoporin Nup35
Protein Family
Nuclear pore complex protein
UniProt Gene Name
NUP35
UniProt Synonym Gene Names
MP44; NUP53; MP-44
UniProt Entry Name
NUP53_HUMAN
NCBI Protein Information
nucleoporin NUP53; mitotic phosphoprotein 44; nuclear pore complex protein Nup53

NCBI Summary for NUP35

This gene encodes a member of the nucleoporin family. The encoded protein contains two membrane binding regions, is localized to the nuclear rim, and is part of the nuclear pore complex. All molecules entering or leaving the nucleus either diffuse through or are actively transported by the nuclear pore complex. Alternative splicing results in multiple transcript variants. Pseudogenes of this gene have been defined on chromosomes 7 and 10. [provided by RefSeq, Dec 2013]
Show more

UniProt Comments for NUP35

NUP35: Functions as a component of the nuclear pore complex (NPC). NPC components, collectively referred to as nucleoporins (NUPs). Can play the role of both NPC structural components and of docking or interaction partners for transiently associated nuclear transport factors. May play a role in the association of MAD1 with the NPC. Belongs to the Nup53 family.

Protein type: Nucleoporin

Chromosomal Location of Human Ortholog: 2q32.1

Cellular Component: nucleoplasm; intermediate filament cytoskeleton; nuclear membrane; intracellular membrane-bound organelle; plasma membrane; nuclear pore; nuclear envelope

Biological Process: mRNA transport; viral reproduction; cytokine and chemokine mediated signaling pathway; mitotic nuclear envelope disassembly; pathogenesis; viral infectious cycle; glucose transport; protein transport; hexose transport; carbohydrate metabolic process; viral transcription; gene expression; mitotic cell cycle; transmembrane transport
Show more

Research Articles on NUP35

1. Observational study of gene-disease association. (HuGE Navigator)

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-NUP35 antibody

 Reference Product  PubMed Publications
 NUP155 antibody  >2 publications with NUP35 and NUP155
 NUP205 antibody  >1 publications with NUP35 and NUP205
 NUP107 antibody  >1 publications with NUP35 and NUP107
 NUP93 antibody  >1 publications with NUP35 and NUP93

Pathways associated with anti-NUP35 antibody

 Products by Pathway  Pathway Diagram
 Antiviral Mechanism By IFN-stimulated Genes Pathway antibodies  Antiviral Mechanism By IFN-stimulated Genes Pathway Diagram
 Cell Cycle Pathway antibodies  Cell Cycle Pathway Diagram
 Cell Cycle, Mitotic Pathway antibodies  Cell Cycle, Mitotic Pathway Diagram
 Cellular Response To Heat Stress Pathway antibodies  Cellular Response To Heat Stress Pathway Diagram
 Cellular Responses To Stress Pathway antibodies  Cellular Responses To Stress Pathway Diagram
 Cytokine Signaling In Immune System Pathway antibodies  Cytokine Signaling In Immune System Pathway Diagram
 Disease Pathway antibodies  Disease Pathway Diagram
 Export Of Viral Ribonucleoproteins From Nucleus Pathway antibodies  Export Of Viral Ribonucleoproteins From Nucleus Pathway Diagram
 Gene Expression Pathway antibodies  Gene Expression Pathway Diagram
 Glucose Transport Pathway antibodies  Glucose Transport Pathway Diagram
Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000