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COBRA1 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS3902344

COBRA1 (Cofactor of BRCA1) is also known as NELFB (negative elongation factor E). NELFB is one of four subunits that make up the NELF transcription elongation factor. The four subunits of NELF are NELF A (WHSC2), NELFB, either NELFC or NELFD, and NELFE. NELF,… More details

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Product Overview

Online Datasheet in PDF
Product Name
COBRA1, Polyclonal Antibody
Full Product Name
Rabbit anti-COBRA1 Antibody, Affinity Purified
Product Gene Name
anti-COBRA1 antibody
Product Synonym Gene Name
NELFB
Product Synonym Names
cofactor of BRCA1; negative elongation factor B; negative elongation factor protein B; NELF-B; COBRA1; Negative elongation factor B; negative elongation factor complex member B
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human
Form/Format
Liquid. Whole IgG. Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Purity/Purification
Antigen Affinity Purified
Concentration
200ug/ml (lot specific)
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Molecular Weight
65,697 Da
OMIM
611180
3D Structure
ModBase 3D Structure for Q8WX92
Immunogen
Between 530 and C-terminus
Conjugation
Unconjugated

Product Categories/Family for anti-COBRA1 antibody

Primary Antibodies
Applications Tested/Suitable for anti-COBRA1 antibody
Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC)
Application Notes for anti-COBRA1 antibody
WB: 1:2000-1:10,000
IP: 6ug/mg lysate
IHC: 1:200-1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

Immunohistochemistry (IHC) of anti-COBRA1 antibody

Detection of human COBRA1 by immunohistochemistry. Sample: FFPE section of human placenta. Antibody: Affinity purified rabbit anti-COBRA1 (Cat. No. MBS3902344) used at a dilution of 1:200 (1ug/ml). Detection: DAB
Rabbit Anti-COBRA1 Polyclonal Antibody – Cat# MBS3902344

Western Blot (WB) of anti-COBRA1 antibody

Detection of human COBRA1 by western blot and immunoprecipitation. Samples: Whole cell lysate (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-COBRA1 antibody MBS3902344 used for WB at 0.04 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. COBRA1 was also immunoprecipitated by rabbit anti-COBRA1 antibody A301-911A, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).
Rabbit Anti-COBRA1 Polyclonal Antibody, Western Blot (WB) – Cat# MBS3902344

Other Notes

Small volumes of anti-COBRA1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for COBRA1 purchase

MBS3902344 is an antibody targeting COBRA1. Also known as Rabbit anti-COBRA1 Antibody, Affinity Purified. Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for COBRA1. It may not necessarily be applicable to this product.

NCBI GI #
862669351
NCBI GeneID
25920
NCBI Accession #
NP_056271.3
UniProt Primary Accession #
Q8WX92
UniProt Secondary Accession #
Q96EW5; Q9H9R4; Q9ULN8; Q9Y3W0; A2BFA3
UniProt Related Accession #
Q8WX92
GenBank Nucleotide #
NM_015456.4
NCBI Official Full Name
negative elongation factor B
NCBI Official Synonym Full Names
negative elongation factor complex member B
NCBI Official Symbol
NELFB
NCBI Official Synonym Symbols
COBRA1; NELF-B
UniProt Protein Name
Negative elongation factor B
UniProt Synonym Protein Names
Cofactor of BRCA1
UniProt Gene Name
NELFB
UniProt Synonym Gene Names
COBRA1; KIAA1182; NELF-B
UniProt Entry Name
NELFB_HUMAN
NCBI Protein Information
negative elongation factor B

NCBI Summary for COBRA1

NELFB is a subunit of negative elongation factor (NELF), which also includes NELFA (WHSC2; MIM 606026), either NELFC or NELFD (TH1L; MIM 605297), and NELFE (RDBP; MIM 154040). NELF acts with DRB sensitivity-inducing factor (DSIF), a heterodimer of SPT4 (SUPT4H1; MIM 603555) and SPT5 (SUPT5H; MIM 602102), to cause transcriptional pausing of RNA polymerase II (see MIM 180660) (Narita et al., 2003 [PubMed 12612062]).[supplied by OMIM, Mar 2008]
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UniProt Comments for COBRA1

COBRA1: a subunit of the multisubunit transcription elongation factor NELF (negative elongation factor). May be involved in transcriptional pausing of RNA polymerase II (RNAPII). Interacts with the first BRCT repeat of BRCA1.

Protein type: Nuclear receptor co-regulator; Transcription, coactivator/corepressor

Chromosomal Location of Human Ortholog: 9q34

Cellular Component: nucleoplasm; cytoplasm

Molecular Function: protein binding

Biological Process: transcription from RNA polymerase II promoter; viral reproduction; positive regulation of viral transcription; RNA elongation from RNA polymerase II promoter; gene expression; negative regulation of transcription, DNA-dependent
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Research Articles on COBRA1

1. These studies allow us to position the actions of two new modulators of GR-regulated transactivation, NELF-A and NELF-B, relative to other factors in the overall gene induction sequence.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-COBRA1 antibody

 Products by Pathway  Pathway Diagram
 Abortive Elongation Of HIV-1 Transcript In The Absence Of Tat Pathway antibodies  Abortive Elongation Of HIV-1 Transcript In The Absence Of Tat Pathway Diagram
 Disease Pathway antibodies  Disease Pathway Diagram
 Formation Of HIV Elongation Complex In The Absence Of HIV Tat Pathway antibodies  Formation Of HIV Elongation Complex In The Absence Of HIV Tat Pathway Diagram
 Formation Of HIV-1 Elongation Complex Containing HIV-1 Tat Pathway antibodies  Formation Of HIV-1 Elongation Complex Containing HIV-1 Tat Pathway Diagram
 Formation Of RNA Pol II Elongation Complex Pathway antibodies  Formation Of RNA Pol II Elongation Complex Pathway Diagram
 Formation Of The Early Elongation Complex Pathway antibodies  Formation Of The Early Elongation Complex Pathway Diagram
 Formation Of The HIV-1 Early Elongation Complex Pathway antibodies  Formation Of The HIV-1 Early Elongation Complex Pathway Diagram
 Gene Expression Pathway antibodies  Gene Expression Pathway Diagram
 HIV Infection Pathway antibodies  HIV Infection Pathway Diagram
 HIV Life Cycle Pathway antibodies  HIV Life Cycle Pathway Diagram
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