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GEF-H1 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS3902359

GEF-H1 (guanine nucleotide exchange factor H1) is a microtubule regulated guanine nucleotide exchange factor for the RhoA GTPase. It catalyzes the exchange of GDP for GTP by Rho in response to microtubule destabilization. GEF-H1 has shown to be regulated… More details

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Product Overview

Online Datasheet in PDF
Product Name
GEF-H1, Polyclonal Antibody
Full Product Name
Rabbit anti-GEF-H1 Antibody, Affinity Purified
Product Gene Name
anti-GEF-H1 antibody
Product Synonym Gene Name
ARHGEF2
Product Synonym Names
NEDMHM; GEFH1; Rho/Rac guanine nucleotide exchange factor (GEF) 2; rho guanine nucleotide exchange factor 2; proliferating cell nucleolar antigen p40; P40; LFP40; GEF; GEF-H1; microtubule-regulated Rho-GEF; guanine nucleotide exchange factor H1; Rho guanine nucleotide exchange factor 2; Rho/Rac guanine nucleotide exchange factor 2
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Purity/Purification
Antigen Affinity Purified
Concentration
1000ug/ml (lot specific)
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Molecular Weight
108,242 Da
OMIM
607560
3D Structure
ModBase 3D Structure for Q92974
Predicted Reactivity
Pig
Immunogen
Between 625 and 675
Conjugation
Unconjugated

Product Categories/Family for anti-GEF-H1 antibody

Primary Antibodies
Applications Tested/Suitable for anti-GEF-H1 antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes for anti-GEF-H1 antibody
WB: 1:2000-1:10,000
IHC: 1:500 to 1:2000. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.

Immunohistochemistry (IHC) of anti-GEF-H1 antibody

Detection of human GEF-H1 by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-GEF-H1 (Cat. No. MBS3902359 lot 2) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB
Rabbit Anti-GEF-H1 Polyclonal Antibody – Cat# MBS3902359

Western Blot (WB) of anti-GEF-H1 antibody

Detection of human GEF-H1 by western blot. Samples: Whole cell lysate (50 ug) from HEK293T, Jurkat, and K-562 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-GEF-H1 antibody MBS3902359 (lot MBS3902359-2) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Rabbit Anti-GEF-H1 Polyclonal Antibody, Western Blot (WB) – Cat# MBS3902359

Other Notes

Small volumes of anti-GEF-H1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for GEF-H1 purchase

MBS3902359 is an antibody targeting GEF-H1. Also known as Rabbit anti-GEF-H1 Antibody, Affinity Purified. Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for GEF-H1. It may not necessarily be applicable to this product.

NCBI GI #
15011974
NCBI GeneID
9181
NCBI Accession #
NP_004714.2
UniProt Primary Accession #
Q92974
UniProt Secondary Accession #
O75142; Q15079; Q5VY92; Q8TDA3; Q8WUG4; Q9H023; D3DVA6
UniProt Related Accession #
Q92974
GenBank Nucleotide #
NM_004723.3
NCBI Official Full Name
rho guanine nucleotide exchange factor 2 isoform 3
NCBI Official Synonym Full Names
Rho/Rac guanine nucleotide exchange factor (GEF) 2
NCBI Official Symbol
ARHGEF2
NCBI Official Synonym Symbols
GEF; P40; GEFH1; LFP40; GEF-H1
UniProt Protein Name
Rho guanine nucleotide exchange factor 2
UniProt Synonym Protein Names
Guanine nucleotide exchange factor H1; GEF-H1; Microtubule-regulated Rho-GEF; Proliferating cell nucleolar antigen p40
Protein Family
Rho guanine nucleotide exchange factor
UniProt Gene Name
ARHGEF2
UniProt Synonym Gene Names
KIAA0651; LFP40; GEF-H1
UniProt Entry Name
ARHG2_HUMAN
NCBI Protein Information
rho guanine nucleotide exchange factor 2; microtubule-regulated Rho-GEF; guanine nucleotide exchange factor H1; proliferating cell nucleolar antigen p40; Rho/Rac guanine nucleotide exchange factor 2

NCBI Summary for GEF-H1

Rho GTPases play a fundamental role in numerous cellular processes that are initiated by extracellular stimuli that work through G protein coupled receptors. The encoded protein may form complex with G proteins and stimulate rho-dependent signals. Alternatively spliced transcript variants encoding different isoforms have been identified.[provided by RefSeq, Jun 2009]
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UniProt Comments for GEF-H1

ARHGEF2: a microtubule-associated guanine nucleotide exchange factor for Rac and Rho GTPases. Mediates cross-talk between microtubules and the actin cytoskeleton. Contains a phorbol esters/diacylglycerol binding and PH domain.

Protein type: GEFs, Rac/Rho; GEFs; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 1q21-q22

Cellular Component: Golgi apparatus; microtubule; protein complex; tight junction; cytoskeleton; focal adhesion; cell soma; cytoplasmic membrane-bound vesicle; cytoplasm; spindle; dendritic shaft; cytosol; vesicle

Molecular Function: Rho guanyl-nucleotide exchange factor activity; protein binding; Rho GTPase binding; zinc ion binding; Rac guanyl-nucleotide exchange factor activity; microtubule binding; Rac GTPase binding; transcription factor binding

Biological Process: mitosis; nerve growth factor receptor signaling pathway; positive regulation of apoptosis; negative regulation of microtubule depolymerization; cell morphogenesis; positive regulation of interleukin-6 production; actin filament organization; positive regulation of tumor necrosis factor production; regulation of cell proliferation; negative regulation of neurogenesis; activation of NF-kappaB transcription factor; intracellular protein transport; regulation of small GTPase mediated signal transduction; positive regulation of peptidyl-tyrosine phosphorylation; establishment of mitotic spindle orientation; cell division; small GTPase mediated signal transduction; regulation of Rho protein signal transduction; innate immune response; positive regulation of transcription from RNA polymerase II promoter
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Research Articles on GEF-H1

1. Paxillin-GEF-H1-p42/44-MAPK module as a regulator of pathological mechanotransduction.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-GEF-H1 antibody

 Reference Product  PubMed Publications
 RHOA antibody  >49 publications with GEF-H1 and RHOA
 RAC1 antibody  >15 publications with GEF-H1 and RAC1
 RHOB antibody  >3 publications with GEF-H1 and RHOB
 MARK2 antibody  >2 publications with GEF-H1 and MARK2
 PAK1 antibody  >2 publications with GEF-H1 and PAK1

Pathways associated with anti-GEF-H1 antibody

 Products by Pathway  Pathway Diagram
 Cell Death Signalling Via NRAGE, NRIF And NADE Pathway antibodies  Cell Death Signalling Via NRAGE, NRIF And NADE Pathway Diagram
 G Alpha (12/13) Signalling Events Pathway antibodies  G Alpha (12/13) Signalling Events Pathway Diagram
 GPCR Downstream Signaling Pathway antibodies  GPCR Downstream Signaling Pathway Diagram
 NRAGE Signals Death Through JNK Pathway antibodies  NRAGE Signals Death Through JNK Pathway Diagram
 Pathogenic Escherichia Coli Infection Pathway antibodies  Pathogenic Escherichia Coli Infection Pathway Diagram
 Pathogenic Escherichia Coli Infection Pathway antibodies  Pathogenic Escherichia Coli Infection Pathway Diagram
 Pathogenic Escherichia Coli Infection Pathway antibodies  Pathogenic Escherichia Coli Infection Pathway Diagram
 Reelin Signaling Pathway antibodies  Reelin Signaling Pathway Diagram
 Regulation Of RAC1 Activity Pathway antibodies  Regulation Of RAC1 Activity Pathway Diagram
 Regulation Of RhoA Activity Pathway antibodies  Regulation Of RhoA Activity Pathway Diagram

Diseases associated with anti-GEF-H1 antibody

 Disease Name  Pubmed Publications
 Inflammation Antibodies  >5 publications with GEF-H1 and Inflammation
 Necrosis Antibodies  >4 publications with GEF-H1 and Necrosis
 Neoplasm Metastasis Antibodies  >3 publications with GEF-H1 and Neoplasm Metastasis
 Breast Neoplasms Antibodies  >3 publications with GEF-H1 and Breast Neoplasms
 Nervous System Diseases Antibodies  >3 publications with GEF-H1 and Nervous System Diseases
 Liver Neoplasms Antibodies  >2 publications with GEF-H1 and Liver Neoplasms
 Liver Diseases Antibodies  >2 publications with GEF-H1 and Liver Diseases
 Fibrosis Antibodies  >1 publications with GEF-H1 and Fibrosis
 Disease Models, Animal Antibodies  >1 publications with GEF-H1 and Disease Models, Animal
 Edema Antibodies  >1 publications with GEF-H1 and Edema

Organs/Tissues associated with anti-GEF-H1 antibody

 Organ/Tissue Name  Pubmed Publications
 Vascular Antibodies  >8 publications with GEF-H1 and Vascular
 Lung Antibodies  >6 publications with GEF-H1 and Lung
 Blood Antibodies  >5 publications with GEF-H1 and Blood
 Kidney Antibodies  >5 publications with GEF-H1 and Kidney
 Brain Antibodies  >5 publications with GEF-H1 and Brain
 Muscle Antibodies  >4 publications with GEF-H1 and Muscle
 Bone Antibodies  >2 publications with GEF-H1 and Bone
 Prostate Antibodies  >2 publications with GEF-H1 and Prostate
 Skin Antibodies  >1 publications with GEF-H1 and Skin
 Eye Antibodies  >1 publications with GEF-H1 and Eye
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