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DICER Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS3902365

Dicer is one of three human RNase III enzymes (Drosha, Dicer, and Argonaute) that plays a diverse role in the processing and maturation of RNA. Dicer is involved in processing pre-cursor mRNAs into both microRNAs (miRNAs) and short interfering RNAs (siRNAs)… More details

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Sample Manual Insert View PDF
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Testing Data View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
Dicer, Polyclonal Antibody
Full Product Name
Rabbit anti-Dicer Antibody, Affinity Purified
Product Gene Name
Product Synonym Gene Name
DICER1
Product Synonym Names
helicase MOI; MNG1; RMSE2; K12H4.8-LIKE; HERNA; helicase with RNAse motif; Dicer1e; Dicer1, Dcr-1 homolog; dicer 1, ribonuclease type III; dicer 1, double-stranded RNA-specific endoribonuclease; Dicer; DCR1; endoribonuclease Dicer; Endoribonuclease Dicer; dicer 1, ribonuclease III
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Purity/Purification
Antigen Affinity Purified
Concentration
1000ug/ml (lot specific)
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Molecular Weight
92,726 Da
OMIM
138800
3D Structure
ModBase 3D Structure for Q9UPY3
Immunogen
Between 1200 and 1250
Conjugation
Unconjugated

Product Categories/Family for anti-Dicer antibody

Applications Tested/Suitable for anti-Dicer antibody
Western Blot (WB), Immunoprecipitation (IP)
Application Notes for anti-Dicer antibody
WB: 1:2000-1:10,000 (3-8% Tris-acetate gel recommended)
IP: 6ug/mg lysate

Immunoprecipitation (IP) of anti-Dicer antibody

Detection of human Dicer by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HEK293T cells. Antibodies: Affinity purified rabbit anti-Dicer antibody MBS3902365 (lot MBS3902365-1 used for IP at 10 ug/mg lysate. Dicer was also immunoprecipitated by rabbit anti-Dicer antibodies MBS3902364 and BL7554, which recognize other epitopes. For blotting immunoprecipitated Dicer, MBS3902365 was used at 1.0 ug/ml. Detection: Chemiluminescence with exposure time of 30 seconds.
Rabbit Anti-DICER Polyclonal Antibody – Cat# MBS3902365

Western Blot (WB) of anti-Dicer antibody

Detection of human Dicer by western blot. Samples: Whole cell lysate from HEK293T (5, 15, and 50 ug) and HeLa (H; 50 ug) cells. Antibody: Affinity purified rabbit anti-Dicer antibody MBS3902365 (lot MBS3902365-1) used at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Rabbit Anti-DICER Polyclonal Antibody, Western Blot (WB) – Cat# MBS3902365

Other Notes

Small volumes of anti-Dicer antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for Dicer purchase

MBS3902365 is an antibody targeting Dicer. Also known as Rabbit anti-Dicer Antibody, Affinity Purified. Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for Dicer. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
endoribonuclease Dicer isoform 1
NCBI Official Synonym Full Names
dicer 1, ribonuclease type III
NCBI Official Symbol
DICER1
NCBI Official Synonym Symbols
DCR1; MNG1; Dicer; HERNA; RMSE2; Dicer1e; K12H4.8-LIKE
UniProt Protein Name
Endoribonuclease Dicer
UniProt Synonym Protein Names
Helicase with RNase motif
Protein Family
UniProt Gene Name
DICER1
UniProt Synonym Gene Names
DICER; HERNA; KIAA0928; Helicase MOI
UniProt Entry Name
DICER_HUMAN
NCBI Protein Information
endoribonuclease Dicer; helicase MOI; Dicer1, Dcr-1 homolog; helicase with RNAse motif; dicer 1, double-stranded RNA-specific endoribonuclease

NCBI Summary for Dicer

This gene encodes a protein possessing an RNA helicase motif containing a DEXH box in its amino terminus and an RNA motif in the carboxy terminus. The encoded protein functions as a ribonuclease and is required by the RNA interference and small temporal RNA (stRNA) pathways to produce the active small RNA component that represses gene expression. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2010]
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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