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RALY Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS3902502

The agouti (a) locus includes the mouse agouti gene that regulates coat color in mice by controlling the relative amount and distribution of yellow and black pigment in the coat hairs. A mutation in the agouti locus, lethal yellow (Ay), results in an… More details

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Product Overview

Online Datasheet in PDF
Product Name
RALY, Polyclonal Antibody
Full Product Name
Rabbit anti-RALY Antibody, Affinity Purified
Product Gene Name
Product Synonym Names
hnRNP associated with lethal yellow protein homolog; RNA-binding protein Raly; RNA-binding protein (autoantigenic, hnRNP-associated with lethal yellow); RNA-binding protein (autoantigenic); RNA binding protein, autoantigenic (hnRNP-associated with lethal yellow homolog); P542; hnRNP core protein C-like 2; heterogeneous nuclear ribonucleoprotein C-like 2; autoantigen p542; HNRPCL2; RNA-binding protein Raly; RALY heterogeneous nuclear ribonucleoprotein
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human, Mouse
Form/Format
Liquid. Whole IgG. Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Purity/Purification
Antigen Affinity Purified
Concentration
200ug/ml (lot specific)
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Molecular Weight
30,364 Da
OMIM
614663
3D Structure
ModBase 3D Structure for Q9UKM9
Immunogen
Between 256 and C-terminus
Conjugation
Unconjugated

Product Categories/Family for anti-RALY antibody

Applications Tested/Suitable for anti-RALY antibody
Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC)
Application Notes for anti-RALY antibody
WB: 1:2000-1:10,000
IP: 6ug/mg lysate
IHC: 1:500 to 1:2000. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.

Immunohistochemistry (IHC) of anti-RALY antibody

Detection of human RALY by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti- RALY (MBS3902502 lot2) used at a dilution of 1:1,000 (0.2 ug/ml).
Rabbit Anti-RALY Polyclonal Antibody – Cat# MBS3902502

Immunohistochemistry (IHC) of anti-RALY antibody

Detection of mouse RALY by immunohistochemistry. Sample: FFPE section of mouse renal cell carcinoma. Antibody: Affinity purified rabbit anti- RALY (MBS3902502 lot 2) used at a dilution of 1:1,000 (0.2 ug/ml).
Rabbit Anti-RALY Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS3902502

Immunoprecipitation (IP) of anti-RALY antibody

Detection of human RALY by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from U2OS cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-RALY antibody (MBS3902502 lot 2) used for IP at 6 ug per reaction. RALY was also immunoprecipitated by a previous lot of this antibody (MBS3902502 lot 1) and a second antibody against a different epitope of RALY (MBS3902486). For blotting immunoprecipitated RALY, MBS3902502 was used at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 1 second.
Rabbit Anti-RALY Polyclonal Antibody, Immunoprecipitation (IP) – Cat# MBS3902502

Western Blot (WB) of anti-RALY antibody

Detection of human RALY by western blot. Samples: Whole cell lysate (10 ug) from Jurkat, HEK293T, Hep-G2, U2OS, and HeLa cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-RALY antibody (MBS3902502 lot 2) used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
Rabbit Anti-RALY Polyclonal Antibody, Western Blot (WB) – Cat# MBS3902502

Other Notes

Small volumes of anti-RALY antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for RALY purchase

MBS3902502 is an antibody targeting RALY. Also known as Rabbit anti-RALY Antibody, Affinity Purified. Reactive in Human, Mouse. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for RALY. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
RNA-binding protein Raly isoform 1
NCBI Official Synonym Full Names
RALY heterogeneous nuclear ribonucleoprotein
NCBI Official Symbol
RALY
NCBI Official Synonym Symbols
P542; HNRPCL2
UniProt Protein Name
RNA-binding protein Raly
UniProt Synonym Protein Names
Autoantigen p542; Heterogeneous nuclear ribonucleoprotein C-like 2; hnRNP core protein C-like 2; hnRNP associated with lethal yellow protein homolog
Protein Family
UniProt Gene Name
RALY
UniProt Synonym Gene Names
HNRPCL2; P542; hnRNP core protein C-like 2
UniProt Entry Name
RALY_HUMAN
NCBI Protein Information
RNA-binding protein Raly

NCBI Summary for RALY

This gene encodes a member of the heterogeneous nuclear ribonucleoprotein (hnRNP) gene family. This protein may play a role in pre-mRNA splicing and in embryonic development. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Sep 2011]

UniProt Comments for RALY

RALY: Probable-RNA binding protein. Could be a heterogeneous nuclear ribonucleoprotein (hnRNP). May be involved in pre-mRNA splicing. Belongs to the RRM HNRPC family. RALY subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Spliceosome; RNA-binding; RNA splicing

Chromosomal Location of Human Ortholog: 20q11.21-q11.23

Cellular Component: nucleus

Molecular Function: protein binding; RNA binding; nucleotide binding

Biological Process: nuclear mRNA splicing, via spliceosome
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-RALY antibody

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