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GIT1 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS3902530

G-protein-coupled receptor kinase interactor 1 (GIT1) and G-protein-coupled receptor kinase interactor 2 (GIT2) are GTPase-activating proteins for ADP-ribosylation (Arf) GTPases. GIT1 and GIT2 regulate Arf activity and the sequestration of activated G… More details

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Product Overview

Online Datasheet in PDF
Product Name
GIT1, Polyclonal Antibody
Full Product Name
Rabbit anti-GIT1 Antibody, Affinity Purified
Product Gene Name
anti-GIT1 antibody
Product Synonym Names
cool-associated and tyrosine-phosphorylated protein 1; G protein-coupled receptor kinase interacting ArfGAP 1; G protein-coupled receptor kinase-interactor 1; CAT1; ARF GAP GIT1; ARF GTPase-activating protein GIT1; CAT-1; GRK-interacting protein 1; ARF GTPase-activating protein GIT1; GIT ArfGAP 1
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human
Form/Format
Liquid. Whole IgG. Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Purity/Purification
Antigen Affinity Purified
Concentration
200ug/ml (lot specific)
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Molecular Weight
85,446 Da
3D Structure
ModBase 3D Structure for Q9Y2X7
Immunogen
Between 375 and 425
Conjugation
Unconjugated

Product Categories/Family for anti-GIT1 antibody

Primary Antibodies
Applications Tested/Suitable for anti-GIT1 antibody
Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC)
Application Notes for anti-GIT1 antibody
WB: 1:2000-1:10,000
IP: 6ug/mg lysate
IHC: 1:100-1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

Immunohistochemistry (IHC) of anti-GIT1 antibody

Detection of human GIT1 by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-GIT1 (Cat. No. MBS3902530 Lot1) used at a dilution of 1:200 (1ug/ml). Detection: DAB
Rabbit Anti-GIT1 Polyclonal Antibody – Cat# MBS3902530

Western Blot (WB) of anti-GIT1 antibody

Detection of human GIT1 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and HEK293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-GIT1 antibody MBS3902530 used for WB at 0.04 ug/ml (A) and 0.4 ug/ml (B) and used for IP at 3 ug/mg lysate. GIT1 was also immunoprecipitated by rabbit anti-GIT1 antibody MBS3902531, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).
Rabbit Anti-GIT1 Polyclonal Antibody, Western Blot (WB) – Cat# MBS3902530

Other Notes

Small volumes of anti-GIT1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for GIT1 purchase

MBS3902530 is an antibody targeting GIT1. Also known as Rabbit anti-GIT1 Antibody, Affinity Purified. Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for GIT1. It may not necessarily be applicable to this product.

NCBI GI #
41393573
NCBI GeneID
28964
NCBI Accession #
NP_054749.2
UniProt Primary Accession #
Q9Y2X7
UniProt Secondary Accession #
Q86SS0; Q9BRJ4; B4DGU9; B4DSV3
UniProt Related Accession #
Q9Y2X7
GenBank Nucleotide #
NM_014030.3
NCBI Official Full Name
ARF GTPase-activating protein GIT1 isoform 2
NCBI Official Synonym Full Names
G protein-coupled receptor kinase interacting ArfGAP 1
NCBI Official Symbol
GIT1
UniProt Protein Name
ARF GTPase-activating protein GIT1
UniProt Synonym Protein Names
G protein-coupled receptor kinase-interactor 1; GRK-interacting protein 1
Protein Family
Adenylate cyclase activation protein
UniProt Gene Name
Git1
UniProt Synonym Gene Names
ARF GAP GIT1
UniProt Entry Name
GIT1_MOUSE
NCBI Protein Information
ARF GTPase-activating protein GIT1; CAT1; CAT-1; ARF GAP GIT1; GRK-interacting protein 1; G protein-coupled receptor kinase interactor 1; G protein-coupled receptor kinase-interactor 1; cool-associated and tyrosine-phosphorylated protein 1

UniProt Comments for GIT1

GIT1: a multidomain, adaptor protein that regulates cellular processes, such as migration and protrusive activity, by bringing together various signaling molecules, including PIX, PAK, and paxillin. Phosphorylation on Ser-709 increases its binding to paxillin and regulates cellular protrusions. Possesses ADP-ribosylation factor (ARF) GTPase-activating protein (GAP) activity. Functions as part of a multi-molecular signaling complex that regulates membrane protrusions and cell migration. Regulates PLC-gamma activation by c-Src and integrates signal transduction by G protein coupled receptors (GPCR)s and tyrosine kinase receptors (TKRs).

Protein type: Motility/polarity/chemotaxis; GAPs; GAPs, ARF

Chromosomal Location of Human Ortholog: 17p11.2

Cellular Component: focal adhesion; membrane; cytoplasm; cytosol

Molecular Function: protein binding; zinc ion binding

Biological Process: axon guidance; ephrin receptor signaling pathway; regulation of cytokinesis; regulation of G-protein coupled receptor protein signaling pathway; positive regulation of GTPase activity
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Research Articles on GIT1

1. miRNA-491-5p and GIT1 serve as modulators and biomarkers for oral squamous cell carcinoma invasion and metastasis.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-GIT1 antibody

 Reference Product  PubMed Publications
 ARF6 antibody  >19 publications with GIT1 and ARF6
 PXN antibody  >17 publications with GIT1 and PXN
 ARHGEF7 antibody  >11 publications with GIT1 and ARHGEF7
 ADRBK1 antibody  >9 publications with GIT1 and ADRBK1
 PTK2 antibody  >7 publications with GIT1 and PTK2
 PTPRZ1 antibody  >4 publications with GIT1 and PTPRZ1
 EPHA2 antibody  >2 publications with GIT1 and EPHA2
 NCK1 antibody  >2 publications with GIT1 and NCK1
 ARHGEF6 antibody  >1 publications with GIT1 and ARHGEF6

Pathways associated with anti-GIT1 antibody

 Products by Pathway  Pathway Diagram
 Arf6 Signaling Events Pathway antibodies  Arf6 Signaling Events Pathway Diagram
 Aurora A Signaling Pathway antibodies  Aurora A Signaling Pathway Diagram
 Axon Guidance Pathway antibodies  Axon Guidance Pathway Diagram
 Developmental Biology Pathway antibodies  Developmental Biology Pathway Diagram
 EGFR1 Signaling Pathway antibodies  EGFR1 Signaling Pathway Diagram
 EPH-Ephrin Signaling Pathway antibodies  EPH-Ephrin Signaling Pathway Diagram
 Endocytosis Pathway antibodies  Endocytosis Pathway Diagram
 Endocytosis Pathway antibodies  Endocytosis Pathway Diagram
 Ephrin Signaling Pathway antibodies  Ephrin Signaling Pathway Diagram
 Epithelial Cell Signaling In Helicobacter Pylori Infection Pathway antibodies  Epithelial Cell Signaling In Helicobacter Pylori Infection Pathway Diagram

Diseases associated with anti-GIT1 antibody

 Disease Name  Pubmed Publications
 Carcinoma Antibodies  >8 publications with GIT1 and Carcinoma
 Attention Deficit Disorder with Hyperactivity Antibodies  >4 publications with GIT1 and Attention Deficit Disorder with Hyperactivity
 Breast Neoplasms Antibodies  >3 publications with GIT1 and Breast Neoplasms
 Inflammation Antibodies  >3 publications with GIT1 and Inflammation
 Lung Neoplasms Antibodies  >3 publications with GIT1 and Lung Neoplasms
 Learning Disorders Antibodies  >2 publications with GIT1 and Learning Disorders
 Hemorrhage Antibodies  >2 publications with GIT1 and Hemorrhage
 Carcinoma, Hepatocellular Antibodies  >2 publications with GIT1 and Carcinoma, Hepatocellular
 Carcinoma, Squamous Cell Antibodies  >2 publications with GIT1 and Carcinoma, Squamous Cell
 Neurologic Manifestations Antibodies  >2 publications with GIT1 and Neurologic Manifestations

Organs/Tissues associated with anti-GIT1 antibody

 Organ/Tissue Name  Pubmed Publications
 Vascular Antibodies  >28 publications with GIT1 and Vascular
 Brain Antibodies  >24 publications with GIT1 and Brain
 Blood Antibodies  >18 publications with GIT1 and Blood
 Muscle Antibodies  >15 publications with GIT1 and Muscle
 Nerve Antibodies  >11 publications with GIT1 and Nerve
 Lung Antibodies  >9 publications with GIT1 and Lung
 Bone Antibodies  >9 publications with GIT1 and Bone
 Embryonic Tissue Antibodies  >6 publications with GIT1 and Embryonic Tissue
 Kidney Antibodies  >6 publications with GIT1 and Kidney
 Stomach Antibodies  >4 publications with GIT1 and Stomach
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