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DAP5 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS3902656

DAP5 (death associated protein 5) is also known as eukaryotic translation initiation factor 4 gamma 2. DAP5 has been found to function as a general repressor of translation and appears to play a role in the switch from cap-dependent to IRES-mediated… More details

$250 / 0.01 mg +1 FREE 8GB USB
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Product Overview

Online Datasheet in PDF
Product Name
DAP5, Polyclonal Antibody
Full Product Name
Rabbit anti-DAP5 Antibody, Affinity Purified
Product Gene Name
anti-DAP5 antibody
Product Synonym Gene Name
EIF4G2
Product Synonym Names
aging-associated protein 1; eIF-4-gamma 2; P97; NAT1; eukaryotic translation initiation factor 4G-like 1; eukaryotic translation initiation factor 4 gamma 2; eIF-4G 2; eIF4G 2; death-associated protein 5; DAP5; AAG1; DAP-5; Eukaryotic translation initiation factor 4 gamma 2; eukaryotic translation initiation factor 4 gamma 2
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Purity/Purification
Antigen Affinity Purified
Concentration
1000ug/ml (lot specific)
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
OMIM
602325
3D Structure
ModBase 3D Structure for P78344
Predicted Reactivity
Mouse, Bovine, Rabbit, Orangutan
Immunogen
Between 125 and 175
Conjugation
Unconjugated

Product Categories/Family for anti-DAP5 antibody

Primary Antibodies
Applications Tested/Suitable for anti-DAP5 antibody
Immunoprecipitation (IP), Immunohistochemistry (IHC)
Application Notes for anti-DAP5 antibody
IP: 6ug/mg lysate
IHC: 1:500-1:2000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

Immunohistochemistry (IHC) of anti-DAP5 antibody

Detection of human DAP5 by immunohistochemistry. Sample: FFPE section of human lung carcinoma. Antibody: Affinity purified rabbit anti-DAP5 (Cat. No. MBS3902656) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB
Rabbit Anti-DAP5 Polyclonal Antibody – Cat# MBS3902656

Immunoprecipitation (IP) of anti-DAP5 antibody

Detection of human DAP5 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-DAP5 antibody MBS3902656 used for IP at 10 ug/mg lysate. DAP5 was also immunoprecipitated by rabbit anti-DAP5 antibody MBS3902655, which recognizes a downstream epitope. For blotting immunoprecipitated DAP5, MBS3902657 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Rabbit Anti-DAP5 Polyclonal Antibody, Immunoprecipitation (IP) – Cat# MBS3902656

Other Notes

Small volumes of anti-DAP5 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for DAP5 purchase

MBS3902656 is an antibody targeting DAP5. Also known as Rabbit anti-DAP5 Antibody, Affinity Purified. Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for DAP5. It may not necessarily be applicable to this product.

NCBI GeneID
1982
NCBI Accession #
NP_001409.3
UniProt Primary Accession #
P78344
UniProt Protein Name
Eukaryotic translation initiation factor 4 gamma 2
UniProt Synonym Protein Names
Death-associated protein 5; DAP-5; p97
UniProt Gene Name
EIF4G2
UniProt Synonym Gene Names
DAP5; eIF-4-gamma 2; eIF-4G 2; eIF4G 2; DAP-5
UniProt Entry Name
IF4G2_HUMAN

UniProt Comments for DAP5

EIF4G2: a general repressor of translation by forming translationally inactive complexes. Appears to play a role in the switch from cap-dependent to IRES-mediated translation during mitosis, apoptosis and viral infection. Interacts with the protein kinases Mnk1 and -2. Binds EIF4A and EIF3. Cleaved by some caspases and viral proteases.

Protein type: Translation initiation; RNA-binding; Translation

Chromosomal Location of Human Ortholog: 11p15

Cellular Component: membrane; eukaryotic translation initiation factor 4F complex; cytosol

Molecular Function: protein binding; translation factor activity, nucleic acid binding; translation initiation factor activity

Biological Process: cell death; cytokine and chemokine mediated signaling pathway; translational initiation; cell cycle arrest; regulation of translational initiation
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
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