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DOCK4 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS3902669

DOCK4 (dedicator of cytokinesis 4) is a member of the CDM gene family and functions as a guanine nucleotide exchange factor (GEF) for the Rac small GTPase. It has been found to play a role in regulating the development of dendrites and rac-dependent cell… More details

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Product Overview

Online Datasheet in PDF
Product Name
DOCK4, Polyclonal Antibody
Full Product Name
Rabbit anti-DOCK4 Antibody, Affinity Purified
Product Gene Name
Product Synonym Names
dedicator of cytokinesis protein 4; Dedicator of cytokinesis protein 4; dedicator of cytokinesis 4
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human
Form/Format
Liquid. Whole IgG. Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Purity/Purification
Antigen Affinity Purified
Concentration
200ug/ml (lot specific)
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Molecular Weight
29,833 Da
OMIM
AY233380 mRNA
3D Structure
ModBase 3D Structure for Q8N1I0
Predicted Reactivity
Mouse
Immunogen
Between 1725 and 1775
Conjugation
Unconjugated

Product Categories/Family for anti-DOCK4 antibody

Applications Tested/Suitable for anti-DOCK4 antibody
Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC)
Application Notes for anti-DOCK4 antibody
WB: 1:2000-1:10,000 (3-8% Tris-acetate gel recommended)
IP: 6ug/mg lysate
IHC: 1:100-1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

Immunohistochemistry (IHC) of anti-DOCK4 antibody

Detection of human DOCK4 by immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-DOCK4 (Cat. No. MBS3902669) used at a dilution of 1:200 (1ug/ml). Detection: DAB
Rabbit Anti-DOCK4 Polyclonal Antibody – Cat# MBS3902669

Western Blot (WB) of anti-DOCK4 antibody

Detection of human DOCK4 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and HEK293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-DOCK4 antibody MBS3902669 used for WB at 0.04 ug/ml (A) and 0.4 ug/ml (B) and used for IP at 3 ug/mg lysate. DOCK4 was also immunoprecipitated by rabbit anti-DOCK4 antibody MBS3902670, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 30 seconds (A and B).
Rabbit Anti-DOCK4 Polyclonal Antibody, Western Blot (WB) – Cat# MBS3902669

Other Notes

Small volumes of anti-DOCK4 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for DOCK4 purchase

MBS3902669 is an antibody targeting DOCK4. Also known as Rabbit anti-DOCK4 Antibody, Affinity Purified. Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for DOCK4. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
dedicator of cytokinesis protein 4
NCBI Official Synonym Full Names
dedicator of cytokinesis 4
NCBI Official Symbol
DOCK4
UniProt Protein Name
Dedicator of cytokinesis protein 4
UniProt Gene Name
DOCK4
UniProt Synonym Gene Names
KIAA0716
NCBI Protein Information
dedicator of cytokinesis protein 4

NCBI Summary for DOCK4

This gene is a member of the dedicator of cytokinesis (DOCK) family and encodes a protein with a DHR-1 (CZH-1) domain, a DHR-2 (CZH-2) domain and an SH3 domain. This membrane-associated, cytoplasmic protein functions as a guanine nucleotide exchange factor and is involved in regulation of adherens junctions between cells. Mutations in this gene have been associated with ovarian, prostate, glioma, and colorectal cancers. Alternatively spliced variants which encode different protein isoforms have been described, but only one has been fully characterized. [provided by RefSeq, Jul 2008]
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UniProt Comments for DOCK4

Involved in regulation of adherens junction between cells. Plays a role in cell migration. Functions as a guanine nucleotide exchange factor (GEF), which activates Rap1 small GTPase by exchanging bound GDP for free GTP.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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