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SASH1 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS3902672

SASH1 (SAM and SH3 domain containing 1) was identified in an in silico analysis of loss of heterozygosity (LOH) to identify genes downregulated in breast cancer. Since this discovery, it has also been found to be a candidate tumor suppressor in colon cancer.… More details

$250 / 0.01 mg +1 FREE 8GB USB
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Sample Manual Insert View PDF
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Testing Data View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
SASH1, Polyclonal Antibody
Full Product Name
Rabbit anti-SASH1 Antibody, Affinity Purified
Product Gene Name
Product Synonym Names
SH3D6A; dJ323M4.1; proline-glutamate repeat-containing protein; SAM and SH3 domain-containing protein 1; SAM and SH3 domain-containing protein 1; SAM and SH3 domain containing 1
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Purity/Purification
Antigen Affinity Purified
Concentration
1000ug/ml (lot specific)
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Molecular Weight
136,653 Da
OMIM
607955
3D Structure
ModBase 3D Structure for O94885
Immunogen
Between 750 and 800
Conjugation
Unconjugated

Product Categories/Family for anti-SASH1 antibody

Applications Tested/Suitable for anti-SASH1 antibody
Immunoprecipitation (IP), Immunohistochemistry (IHC), Immunohistochemistry-Immunofluorescence (IHC-IF)
Application Notes for anti-SASH1 antibody
IP: 6ug/mg lysate
IHC: 1:500-1:2000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
IHC-IF: 1:250-1:1000

Immunohistochemistry (IHC) of anti-SASH1 antibody

Detection of human SASH1 by immunohistochemistry. Sample: FFPE sections of human breast carcinoma (left) and lung carcinoma (right). Antibody: Affinity purified rabbit anti-SASH1 (Cat. No. MBS3902672 Lot1) used at a dilution of 1:1,000 (1ug/ml) and 1:400 (2.5 ug/ml). Detection: DAB and Red-fluorescent goat anti-rabbit IgG-heavy and light chain, cross-adsorbed Antibody DyLight 594 Conjugated used at a dilution of 1:100.
Rabbit Anti-SASH1 Polyclonal Antibody – Cat# MBS3902672

Immunoprecipitation (IP) of anti-SASH1 antibody

Detection of human SASH1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-SASH1 antibody MBS3902672 used for IP at 3 ug/mg lysate. SASH1 was also immunoprecipitated by rabbit anti-SASH1 antibody MBS3902671, which recognizes an upstream epitope. For blotting immunoprecipitated SASH1, A320-265A was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Rabbit Anti-SASH1 Polyclonal Antibody, Immunoprecipitation (IP) – Cat# MBS3902672

Other Notes

Small volumes of anti-SASH1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for SASH1 purchase

MBS3902672 is an antibody targeting SASH1. Also known as Rabbit anti-SASH1 Antibody, Affinity Purified. Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for SASH1. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
SAM and SH3 domain-containing protein 1
NCBI Official Synonym Full Names
SAM and SH3 domain containing 1
NCBI Official Symbol
SASH1
NCBI Official Synonym Symbols
SH3D6A; dJ323M4; dJ323M4.1
UniProt Protein Name
SAM and SH3 domain-containing protein 1
UniProt Synonym Protein Names
Proline-glutamate repeat-containing protein
UniProt Gene Name
SASH1
UniProt Synonym Gene Names
KIAA0790; PEPE1
UniProt Entry Name
SASH1_HUMAN
NCBI Protein Information
SAM and SH3 domain-containing protein 1; 2500002E12Rik; proline-glutamate repeat-containing protein

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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