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MED15 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS3902811

Mediator complex subunit 15 is a component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory… More details

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Product Overview

Online Datasheet in PDF
Product Name
MED15, Polyclonal Antibody
Full Product Name
Rabbit anti-MED15 Antibody, Affinity Purified
Product Gene Name
anti-MED15 antibody
Product Synonym Names
trinucleotide repeat-containing gene 7 protein; positive cofactor 2, glutamine/Q-rich-associated protein; TIG1; TIG-1; TNRC7; TPA inducible gene-1; TPA inducible protein; trinucleotide repeat containing 7; CTG7A; Positive cofactor 2 glutamine/Q-rich-associated protein; TPA-inducible gene 1 protein; CAG7A; PCQAP; activator-recruited cofactor 105 kDa component; mediator of RNA polymerase II transcription subunit 15; ARC105; CTG repeat protein 7a; Mediator complex subunit 15; PC2 (positive cofactor 2, multiprotein complex) glutamine/Q-rich-associated protein; PC2 glutamine/Q-rich-associated protein; PC2-glutamine-rich-associated protein; activator-recruited cofactor, 105-kD; Mediator of RNA polymerase II transcription subunit 15; mediator complex subunit 15
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human
Form/Format
Liquid. Whole IgG. Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Purity/Purification
Antigen Affinity Purified
Concentration
200ug/ml (lot specific)
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Molecular Weight
86,753 Da
Chromosome Location
Chromosome: 22; NC_000022.10 (20861886..20941919). Location: 22q11.2
OMIM
607372
3D Structure
ModBase 3D Structure for Q96RN5
Predicted Reactivity
Mouse
Immunogen
Between 675 and 725
Conjugation
Unconjugated

Product Categories/Family for anti-MED15 antibody

Primary Antibodies
Applications Tested/Suitable for anti-MED15 antibody
Western Blot (WB), Immunoprecipitation (IP)
Application Notes for anti-MED15 antibody
WB: 1:2000-1:10,000
IP: 6ug/mg lysate

Immunoprecipitation (IP) of anti-MED15 antibody

Detection of human MED15 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-MED15 antibody MBS3902811 (lot MBS3902811-2) used for IP at 3 ug per reaction. MED15 was also immunoprecipitated by rabbit anti-MED15 antibody MBS3902810. For blotting immunoprecipitated MED15, MBS3902811 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.
Rabbit Anti-MED15 Polyclonal Antibody – Cat# MBS3902811

Western Blot (WB) of anti-MED15 antibody

Detection of human MED15 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-MED15 antibody MBS3902811 (lot MBS3902811-2) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Rabbit Anti-MED15 Polyclonal Antibody, Western Blot (WB) – Cat# MBS3902811

Other Notes

Small volumes of anti-MED15 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for MED15 purchase

MBS3902811 is an antibody targeting MED15. Also known as Rabbit anti-MED15 Antibody, Affinity Purified. Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for MED15. It may not necessarily be applicable to this product.

NCBI GI #
51477700
NCBI GeneID
51586
NCBI Accession #
NP_001003891.1
UniProt Primary Accession #
Q96RN5
UniProt Secondary Accession #
O15413; Q6IC31; Q8NF16; Q96CT0; Q96IH7; Q9P1T3; D3DX31; D3DX32
UniProt Related Accession #
Q96RN5
GenBank Nucleotide #
NM_001003891.1
NCBI Official Full Name
mediator of RNA polymerase II transcription subunit 15 isoform a
NCBI Official Synonym Full Names
mediator complex subunit 15
NCBI Official Symbol
MED15
NCBI Official Synonym Symbols
TIG1; CAG7A; CTG7A; PCQAP; TIG-1; TNRC7; ARC105
UniProt Protein Name
Mediator of RNA polymerase II transcription subunit 15
UniProt Synonym Protein Names
Activator-recruited cofactor 105 kDa component; ARC105; CTG repeat protein 7a; Mediator complex subunit 15; Positive cofactor 2 glutamine/Q-rich-associated protein; PC2 glutamine/Q-rich-associated protein; TPA-inducible gene 1 protein; TIG-1; Trinucleotide repeat-containing gene 7 protein
Protein Family
Mediator of RNA polymerase II transcription
UniProt Gene Name
MED15
UniProt Synonym Gene Names
ARC105; CTG7A; PCQAP; TIG1; TNRC7; ARC105; PC2 glutamine/Q-rich-associated protein; TIG-1
UniProt Entry Name
MED15_HUMAN
NCBI Protein Information
mediator of RNA polymerase II transcription subunit 15; TPA inducible gene-1; CTG repeat protein 7a; TPA inducible protein; TPA-inducible gene 1 protein; trinucleotide repeat containing 7; activator-recruited cofactor, 105-kD; PC2-glutamine-rich-associate

UniProt Comments for MED15

PCQAP: Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. Required for cholesterol-dependent gene regulation. Positively regulates the Nodal signaling pathway. Belongs to the Mediator complex subunit 15 family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Transcription, coactivator/corepressor; Nuclear receptor co-regulator

Chromosomal Location of Human Ortholog: 22q11.2

Cellular Component: nucleoplasm; membrane; cytoplasm; Srb-mediator complex; nucleus

Molecular Function: protein binding

Biological Process: regulation of transcription from RNA polymerase II promoter; transcription initiation from RNA polymerase II promoter; stem cell maintenance; gene expression
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-MED15 antibody

 Reference Product  PubMed Publications
 TRIM11 antibody  >1 publications with MED15 and TRIM11
 SMAD3 antibody  >1 publications with MED15 and SMAD3
 SMAD2 antibody  >1 publications with MED15 and SMAD2

Pathways associated with anti-MED15 antibody

 Products by Pathway  Pathway Diagram
 Diabetes Pathways antibodies  Diabetes Pathways Diagram
 Gene Expression Pathway antibodies  Gene Expression Pathway Diagram
 Generic Transcription Pathway antibodies  Generic Transcription Pathway Diagram
 Regulation Of Nuclear SMAD2/3 Signaling Pathway antibodies  Regulation Of Nuclear SMAD2/3 Signaling Pathway Diagram
 Transcriptional Regulation Of White Adipocyte Differentiation Pathway antibodies  Transcriptional Regulation Of White Adipocyte Differentiation Pathway Diagram

Diseases associated with anti-MED15 antibody

 Disease Name  Pubmed Publications
 Pain Antibodies  >7 publications with MED15 and Pain
 Arthritis, Rheumatoid Antibodies  >4 publications with MED15 and Arthritis, Rheumatoid
 Inflammation Antibodies  >3 publications with MED15 and Inflammation
 Colitis Antibodies  >1 publications with MED15 and Colitis
 Gastritis Antibodies  >1 publications with MED15 and Gastritis
 Liver Diseases Antibodies  >1 publications with MED15 and Liver Diseases
 Adenomatous Polyposis Coli Antibodies  >1 publications with MED15 and Adenomatous Polyposis Coli
 Uterine Neoplasms Antibodies  >1 publications with MED15 and Uterine Neoplasms
 Drug-Induced Liver Injury Antibodies  >1 publications with MED15 and Drug-Induced Liver Injury
 Kidney Diseases Antibodies  >1 publications with MED15 and Kidney Diseases

Organs/Tissues associated with anti-MED15 antibody

 Organ/Tissue Name  Pubmed Publications
 Stomach Antibodies  >19 publications with MED15 and Stomach
 Blood Antibodies  >8 publications with MED15 and Blood
 Intestine Antibodies  >6 publications with MED15 and Intestine
 Liver Antibodies  >4 publications with MED15 and Liver
 Embryonic Tissue Antibodies  >4 publications with MED15 and Embryonic Tissue
 Bone Antibodies  >3 publications with MED15 and Bone
 Kidney Antibodies  >2 publications with MED15 and Kidney
 Nerve Antibodies  >1 publications with MED15 and Nerve
 Connective Tissue Antibodies  >1 publications with MED15 and Connective Tissue
 Placenta Antibodies  >1 publications with MED15 and Placenta
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