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PAP-gamma Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS3902815

Poly(A) polymerase gamma (PAP-gamma) is a member of the poly(A) polymerase family which catalyzes template-independent extension of the 3' end of a DNA/RNA strand. This enzyme shares 60% identity to the well characterized poly(A) polymerase II (PAPII) at the… More details

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Product Overview

Online Datasheet in PDF
Product Name
PAP-gamma, Polyclonal Antibody
Full Product Name
Rabbit anti-PAP-gamma Antibody, Affinity Purified
Product Gene Name
anti-PAP-gamma antibody
Product Synonym Gene Name
PAPOLG
Product Synonym Names
neo-PAP; neo-poly(A) polymerase; nuclear poly(A) polymerase gamma; PAP-gamma; poly(A) polymerase gamma; polynucleotide adenylyltransferase gamma; signal recognition particle RNA-adenylating enzyme; SRP RNA 3' adenylating enzyme/pap2; SRP RNA 3'-adenylating enzyme; SRP RNA-adenylating enzyme; Poly(A) polymerase gamma; poly(A) polymerase gamma
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human, Mouse
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Purity/Purification
Antigen Affinity Purified
Concentration
1000ug/ml (lot specific)
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Molecular Weight
82,803 Da
Chromosome Location
Chromosome: 2; NC_000002.11 (60983365..61029221). Location: 2p16.1
3D Structure
ModBase 3D Structure for Q9BWT3
Immunogen
Between 686 and C-terminus
Conjugation
Unconjugated

Product Categories/Family for anti-PAP-gamma antibody

Primary Antibodies
Applications Tested/Suitable for anti-PAP-gamma antibody
Western Blot (WB), Immunoprecipitation (IP)
Application Notes for anti-PAP-gamma antibody
WB: 1:2000-1:10,000
IP: 6ug/mg lysate

Western Blot (WB) of anti-PAP-gamma antibody

Detection of human and mouse PAP-gamma by western blot (h&m) and immunoprecipitation (h). Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and mouse NIH 3T3 (M; 50 ug) cells. Antibodies: Affinity purified rabbit anti-PAP-gamma antibody MBS3902815 used for WB at 0.1 ug/ml (A) and 1 ug/ml (B) and used for IP at 10 ug/mg lysate. PAP-gamma was also immunoprecipitated by rabbit anti-PAP-gamma antibody MBS3902814, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).
Rabbit Anti-PAP-gamma Polyclonal Antibody – Cat# MBS3902815

Other Notes

Small volumes of anti-PAP-gamma antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for PAP-gamma purchase

MBS3902815 is an antibody targeting PAP-gamma. Also known as Rabbit anti-PAP-gamma Antibody, Affinity Purified. Reactive in Human, Mouse. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for PAP-gamma. It may not necessarily be applicable to this product.

NCBI GI #
16306568
NCBI GeneID
64895
NCBI Accession #
NP_075045.2
UniProt Primary Accession #
Q9BWT3
UniProt Secondary Accession #
Q969N1; Q9H8L2; Q9HAD0; B2RBH4
UniProt Related Accession #
Q9BWT3
GenBank Nucleotide #
NM_022894.3
NCBI Official Full Name
poly(A) polymerase gamma
NCBI Official Synonym Full Names
poly(A) polymerase gamma
NCBI Official Symbol
PAPOLG
UniProt Protein Name
Poly(A) polymerase gamma
UniProt Synonym Protein Names
Neo-poly(A) polymerase; Neo-PAP; Polynucleotide adenylyltransferase gamma; SRP RNA 3'-adenylating enzyme
Protein Family
Poly(A) polymerase
UniProt Gene Name
PAPOLG
UniProt Synonym Gene Names
PAP2; PAPG; PAP-gamma; Neo-PAP
UniProt Entry Name
PAPOG_HUMAN
NCBI Protein Information
poly(A) polymerase gamma; poly(A) polymerase gamma; neo-PAP; PAP-gamma; neo-poly(A) polymerase; SRP RNA 3'-adenylating enzyme; nuclear poly(A) polymerase gamma; SRP RNA 3' adenylating enzyme/pap2; polynucleotide adenylyltransferase gamma

NCBI Summary for PAP-gamma

This gene encodes a member of the poly(A) polymerase family which catalyzes template-independent extension of the 3' end of a DNA/RNA strand. This enzyme shares 60% identity to the well characterized poly(A) polymerase II (PAPII) at the amino acid level. These two enzymes have similar organization of structural and functional domains. This enzyme is exclusively localized in the nucleus and exhibits both nonspecific and CPSF (cleavage and polyadenylation specificity factor)/AAUAAA-dependent polyadenylation activity. This gene is located on chromosome 2 in contrast to the PAPII gene, which is located on chromosome 14. [provided by RefSeq, Jul 2008]
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UniProt Comments for PAP-gamma

Function: Responsible for the post-transcriptional adenylation of the 3'-terminal of mRNA precursors and several small RNAs including signal recognition particle (SRP) RNA, nuclear 7SK RNA, U2 small nuclear RNA, and ribosomal 5S RNA.

Catalytic activity: ATP + RNA(n) = diphosphate + RNA(n+1).

Cofactor: Binds 2 magnesium ions. Also active with manganese

By similarity.

Subcellular location: Nucleus Ref.2.

Tissue specificity: Expressed predominantly in testis, and weakly in other tissues. Overexpressed in several tumors. Ref.2

Sequence similarities: Belongs to the poly(A) polymerase family.

Sequence caution: The sequence BAB14604.1 differs from that shown. Reason: Erroneous initiation.
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Research Articles on PAP-gamma

1. PAP gamma structurally closely resembles its PAP alpha ortholog. It possesses a conserved catalytic binding pocket, whereas residues at the surface of the polymerase are more divergent.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-PAP-gamma antibody

 Products by Pathway  Pathway Diagram
 MRNA Surveillance Pathway antibodies  MRNA Surveillance Pathway Diagram
 MRNA Surveillance Pathway antibodies  MRNA Surveillance Pathway Diagram

Diseases associated with anti-PAP-gamma antibody

 Disease Name  Pubmed Publications
 Aneuploidy Antibodies  >1 publications with PAP-gamma and Aneuploidy

Organs/Tissues associated with anti-PAP-gamma antibody

 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >1 publications with PAP-gamma and Brain
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