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SPF45 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS3902875

Splicing factor 45 (SPF45) is an RNA splicing factor that binds to the single stranded 3'AG at the exon/intron border and promotes its utilization in the second catalytic step. SPF45 is involved in the regulation of alternative splicing and the utilization of… More details

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Product Overview

Online Datasheet in PDF
Product Name
SPF45, Polyclonal Antibody
Full Product Name
Rabbit anti-SPF45 Antibody, Affinity Purified
Product Gene Name
Product Synonym Gene Name
RBM17
Product Synonym Names
45 kDa-splicing factor; RNA-binding motif protein 17; SPF45; splicing factor 45; splicing factor 45kDa; Splicing factor 45; RNA binding motif protein 17
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human, Mouse
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Purity/Purification
Antigen Affinity Purified
Concentration
1000ug/ml (lot specific)
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Molecular Weight
44 kDa
OMIM
606935
3D Structure
ModBase 3D Structure for Q96I25
Immunogen
Between 25 and 75
Conjugation
Unconjugated

Product Categories/Family for anti-SPF45 antibody

Applications Tested/Suitable for anti-SPF45 antibody
Immunoprecipitation (IP), Immunohistochemistry (IHC)
Application Notes for anti-SPF45 antibody
IP: 6ug/mg lysate
IHC: 1:1000-1:5000. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.

Immunohistochemistry (IHC) of anti-SPF45 antibody

Detection of mouse SPF45 by immunohistochemistry. Sample: FFPE section of mouse plasmacytoma. Antibody: Affinity purified rabbit anti-SPF45 (Cat. No. MBS3902875 lot 3) used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB
Rabbit Anti-SPF45 Polyclonal Antibody – Cat# MBS3902875

Immunoprecipitation (IP) of anti-SPF45 antibody

Detection of human SPF45 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-SPF45 antibody MBS3902875 (lot MBS3902875-3) used for IP at 6 ug per reaction. SPF45 was also immunoprecipitated by a previous lot of this antibody (lot MBS3902875-2) and rabbit anti-SPF45 antibody MBS3902876. For blotting immunoprecipitated SPF45, MBS3902875 was used at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Rabbit Anti-SPF45 Polyclonal Antibody, Immunoprecipitation (IP) – Cat# MBS3902875

Other Notes

Small volumes of anti-SPF45 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for SPF45 purchase

MBS3902875 is an antibody targeting SPF45. Also known as Rabbit anti-SPF45 Antibody, Affinity Purified. Reactive in Human, Mouse. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for SPF45. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Synonym Full Names
RNA binding motif protein 17
NCBI Official Symbol
RBM17
NCBI Official Synonym Symbols
SPF45
UniProt Protein Name
Splicing factor 45
UniProt Synonym Protein Names
45 kDa-splicing factor; RNA-binding motif protein 17
UniProt Gene Name
RBM17
UniProt Synonym Gene Names
SPF45
UniProt Entry Name
SPF45_HUMAN
NCBI Protein Information
splicing factor 45

NCBI Summary for SPF45

This gene encodes an RNA binding protein. The encoded protein is part of the spliceosome complex and functions in the second catalytic step of mRNA splicing. Alternatively spliced transcript variants have been described. Related pseudogenes exist on chromosomes 9 and 15. [provided by RefSeq, Mar 2009]
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UniProt Comments for SPF45

RBM17: Splice factor that binds to the single stranded 3'AG at the exon/intron border and promotes its utilization in the second catalytic step. Involved in the regulation of alternative splicing and the utilization of cryptic splice sites. Promotes the utilization of a cryptic splice site created by the beta-110 mutation in the HBB gene. The resulting frameshift leads to sickle cell anemia.

Protein type: Spliceosome; RNA-binding; RNA splicing

Chromosomal Location of Human Ortholog: 10p15.1

Cellular Component: nucleoplasm; spliceosome

Molecular Function: protein binding; RNA binding; nucleotide binding

Biological Process: alternative nuclear mRNA splicing, via spliceosome
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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