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NEDD4L Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS3902890

NEDD4L (neural precursor cell expressed, developmentally down-regulated 4-like) functions as an E3 ubiquitin-protein ligase. NEDD4L inhibits TGF-beta signaling by triggering SMAD2 and TGFR1 ubiquitination and proteasome-dependent degradation. It also promotes… More details

$250 / 0.002 mg +1 FREE 8GB USB
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Testing Data View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
NEDD4L, Polyclonal Antibody
Full Product Name
Rabbit anti-NEDD4L Antibody, Affinity Purified
Product Gene Name
Product Synonym Names
E3 ubiquitin-protein ligase NEDD4-like; PVNH7; RSP5; ubiquitin-protein ligase Rsp5; NEDD4.2; HECT-type E3 ubiquitin transferase NED4L; hNEDD4-2; NEDD4-2; E3 ubiquitin-protein ligase NEDD4-like; neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human, Mouse
Form/Format
Liquid. Whole IgG. Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Purity/Purification
Antigen Affinity Purified
Concentration
200ug/ml (lot specific)
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
OMIM
AF210730 mRNA
3D Structure
ModBase 3D Structure for Q96PU5
Predicted Reactivity
Orangutan
Immunogen
Between 300 and 350
Conjugation
Unconjugated

Product Categories/Family for anti-NEDD4L antibody

Applications Tested/Suitable for anti-NEDD4L antibody
Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC)
Application Notes for anti-NEDD4L antibody
WB: 1:2000-1:10,000
IP: 6ug/mg lysate
IHC: 1:100-1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

Immunohistochemistry (IHC) of anti-NEDD4L antibody

Detection of human NEDD4L by immunohistochemistry. Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-NEDD4L (Cat. No. MBS3902890 Lot1) used at a dilution of 1:200 (1ug/ml). Detection: DAB
Rabbit Anti-NEDD4L Polyclonal Antibody – Cat# MBS3902890

Western Blot (WB) of anti-NEDD4L antibody

Detection of human and mouse NEDD4L by western blot (h&m) and immunoprecipitation (h). Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), HEK293T (T; 50 ug), and mouse NIH 3T3 (M; 50 ug) cells. Antibodies: Affinity purified rabbit anti-NEDD4L antibody MBS3902890 used for WB at 0.04 ug/ml (A) and 0.4 ug/ml (B) and used for IP at 3 ug/mg lysate. NEDD4L was also immunoprecipitated by rabbit anti-NEDD4L antibodies MBS3902889 and MBS3902891, which recognize other epitopes. Detection: Chemiluminescence with exposure times of 10 second (A) and 3 seconds (B).
Rabbit Anti-NEDD4L Polyclonal Antibody, Western Blot (WB) – Cat# MBS3902890

Other Notes

Small volumes of anti-NEDD4L antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for NEDD4L purchase

MBS3902890 is an antibody targeting NEDD4L. Also known as Rabbit anti-NEDD4L Antibody, Affinity Purified. Reactive in Human, Mouse. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for NEDD4L. It may not necessarily be applicable to this product.

NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Protein Name
E3 ubiquitin-protein ligase NEDD4-like
UniProt Synonym Protein Names
HECT-type E3 ubiquitin transferase NED4L; NEDD4.2; Nedd4-2
UniProt Gene Name
NEDD4L
UniProt Synonym Gene Names
KIAA0439; NEDL3

UniProt Comments for NEDD4L

E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Inhibits TGF-beta signaling by triggering SMAD2 and TGFBR1 ubiquitination and proteasome-dependent degradation. Promotes ubiquitination and internalization of various plasma membrane channels such as ENaC, Nav1.2, Nav1.3, Nav1.5, Nav1.7, Nav1.8, Kv1.3, KCNH2, EAAT1 or CLC5 (PubMed:26363003). Promotes ubiquitination and degradation of SGK1 and TNK2. Ubiquitinates BRAT1 and this ubiquitination is enhanced in the presence of NDFIP1 (PubMed:25631046). Plays a role in dendrite formation by melanocytes (PubMed:23999003). Involved in the regulation of TOR signaling (PubMed:27694961).
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
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