Rabbit Anti-p115-RhoGEF Polyclonal Antibody – Cat# MBS3903056

Detection of human p115-RhoGEF by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-p115-RhoGEF antibody MBS3903056 used for IP at 3 ug/mg lysate. p115-RhoGEF was also immunoprecipitated by rabbit anti-p115-RhoGEF antibody MBS3903055, which recognizes an upstream epitope. For blotting immunoprecipitated p115-RhoGEF, MBS3903055 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.

p115-RhoGEF Antibody – Rabbit Polyclonal

Name: p115-RhoGEF Antibody; Polyclonal; Rabbit; Human-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS3903056
Price: 250 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS3903056

P115-RhoGEF plays a role in the regulation of RhoA GTPase by guanine nucleotide-binding alpha-12 (GNA12) and alpha-13 (GNA13) subunits. P115-RhoGEF acts as a GTPase-activating protein (GAP) for GNA12 and GNA13, and as guanine nucleotide exchange factor (GEF)… More details

$250 / 0.01 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

p115-RhoGEF, Polyclonal Antibody

Full Product Name

Rabbit anti-p115-RhoGEF Antibody, Affinity Purified

Product Gene Name

anti-p115-RhoGEF antibody

Product Synonym Gene Name

ARHGEF1

Product Synonym Names

115 kDa guanine nucleotide exchange factor; p115RhoGEF; rho guanine nucleotide exchange factor 1; SUB1.5; Rho guanine nucleotide exchange factor (GEF) 1; P115-RHOGEF; Lsc homolog; LSC; LBCL2; 115-kD protein; GEF1; Rho guanine nucleotide exchange factor 1; Rho guanine nucleotide exchange factor 1

Clonality

Polyclonal

Host

Rabbit

Species Reactivity

Human

Form/Format

Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide

Purity/Purification

Antigen Affinity Purified

Concentration

1000ug/ml (lot specific)

Preparation and Storage

Store at 2-8 degree C
Shelf Life: 1 year from date of receipt

OMIM

601855

3D Structure

ModBase 3D Structure for Q92888

Immunogen

Between 775 and 825

Conjugation

Unconjugated

Product Categories/Family for anti-p115-RhoGEF antibody

Primary Antibodies

Applications Tested/Suitable for anti-p115-RhoGEF antibody

Immunoprecipitation (IP)

Application Notes for anti-p115-RhoGEF antibody

IP: 6ug/mg lysate

Other Notes

Small volumes of anti-p115-RhoGEF antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for p115-RhoGEF purchase

MBS3903056 is an antibody targeting p115-RhoGEF. Also known as Rabbit anti-p115-RhoGEF Antibody, Affinity Purified. Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for p115-RhoGEF. It may not necessarily be applicable to this product.

NCBI GeneID

9138

NCBI Accession #

NP_945328.1

UniProt Primary Accession #

Q92888

UniProt Protein Name

Rho guanine nucleotide exchange factor 1

UniProt Synonym Protein Names

115 kDa guanine nucleotide exchange factor; p115-RhoGEF; p115RhoGEF; Sub1.5

Protein Family

Rho guanine nucleotide exchange factor

UniProt Gene Name

ARHGEF1

UniProt Synonym Gene Names

p115-RhoGEF; p115RhoGEF

UniProt Entry Name

ARHG1_HUMAN

UniProt Comments for p115-RhoGEF

ARHGEF1: Seems to play a role in the regulation of RhoA GTPase by guanine nucleotide-binding alpha-12 (GNA12) and alpha-13 (GNA13) subunits. Acts as GTPase-activating protein (GAP) for GNA12 and GNA13, and as guanine nucleotide exchange factor (GEF) for RhoA GTPase. Activated G alpha 13/GNA13 stimulates the RhoGEF activity through interaction with the RGS-like domain. This GEF activity is inhibited by binding to activated GNA12. Mediates angiotensin-2- induced RhoA activation. Interacts with RHOA, GNA12 and GNA13. Homooligomerizes through the coiled coil region. May interact with CCPG1. Interacts with CTNNAL1. Ubiquitously expressed. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: GEFs, Rac/Rho; GEFs; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 19q13.13

Cellular Component: cytoplasm; plasma membrane; cytosol

Molecular Function: Rho guanyl-nucleotide exchange factor activity; protein binding; GTPase activator activity

Biological Process: cell proliferation; nerve growth factor receptor signaling pathway; negative regulation of axonogenesis; regulation of axonogenesis; Rho protein signal transduction

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.