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NOP56 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS3903072

Nucleolar protein 56 (NOP56) is a core component of the box C/D snoRNP complex. The snoRNP core complex is made up of NOP58, NOP56, and fibrillin and is involved in ribosome biogenesis. More details

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Sample Manual Insert View PDF
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Testing Data View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
NOP56, Polyclonal Antibody
Full Product Name
Rabbit anti-NOP56 Antibody, Affinity Purified
Product Gene Name
Product Synonym Names
NOL5A; NOP56 ribonucleoprotein homolog; nucleolar protein 56; Nucleolar protein 5A; nucleolar protein 5A (56kDa with KKE/D repeat); SCA36; Nucleolar protein 56; NOP56 ribonucleoprotein
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human, Mouse
Form/Format
Liquid. Whole IgG. Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Purity/Purification
Antigen Affinity Purified
Concentration
200ug/ml (lot specific)
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Molecular Weight
66,050 Da
OMIM
614153
3D Structure
ModBase 3D Structure for O00567
Immunogen
Between 525 and 575
Conjugation
Unconjugated

Product Categories/Family for anti-NOP56 antibody

Applications Tested/Suitable for anti-NOP56 antibody
Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC)
Application Notes for anti-NOP56 antibody
WB: 1:2000-1:10,000
IP: 6ug/mg lysate
IHC: 1:100-1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

Immunohistochemistry (IHC) of anti-NOP56 antibody

Detection of human NOP56 by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-NOP56 (Cat. No. MBS3903072 Lot1) used at a dilution of 1:200 (1ug/ml). Detection: DAB
Rabbit Anti-NOP56 Polyclonal Antibody – Cat# MBS3903072

Western Blot (WB) of anti-NOP56 antibody

Detection of human and mouse NOP56 by western blot (h&m) and immunoprecipitation (h). Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), HEK293T (T; 50 ug), and mouse NIH 3T3 (M; 50 ug) cells. Antibodies: Affinity purified rabbit anti-NOP56 antibody MBS3903072 used for WB at 0.04 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. NOP56 was also immunoprecipitated by rabbit anti-NOP56 antibody MBS3903073, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 10 seconds (B).
Rabbit Anti-NOP56 Polyclonal Antibody, Western Blot (WB) – Cat# MBS3903072

Other Notes

Small volumes of anti-NOP56 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for NOP56 purchase

MBS3903072 is an antibody targeting NOP56. Also known as Rabbit anti-NOP56 Antibody, Affinity Purified. Reactive in Human, Mouse. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for NOP56. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
nucleolar protein 56
NCBI Official Synonym Full Names
NOP56 ribonucleoprotein
NCBI Official Symbol
NOP56
NCBI Official Synonym Symbols
NOL5A; SCA36
UniProt Protein Name
Nucleolar protein 56
UniProt Synonym Protein Names
Nucleolar protein 5A
Protein Family
UniProt Gene Name
NOP56
UniProt Synonym Gene Names
NOL5A
NCBI Protein Information
nucleolar protein 56

NCBI Summary for NOP56

Nop56p is a yeast nucleolar protein that is part of a complex with the nucleolar proteins Nop58p and fibrillarin. Nop56p is required for assembly of the 60S ribosomal subunit and is involved in pre-rRNA processing. The protein encoded by this gene is similar in sequence to Nop56p and is also found in the nucleolus. Expansion of a GGCCTG repeat from 3-8 copies to 1500-2500 copies in an intron of this gene results in spinocerebellar ataxia 36. Multiple transcript variants encoding several different isoforms have been found for this gene, but the full-length nature of most of them has not been determined. [provided by RefSeq, Jul 2016]
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UniProt Comments for NOP56

Involved in the early to middle stages of 60S ribosomal subunit biogenesis. Core component of box C/D small nucleolar ribonucleoprotein (snoRNP) particles. Required for the biogenesis of box C/D snoRNAs such U3, U8 and U14 snoRNAs.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-NOP56 antibody

 Reference Product  PubMed Publications
 TCOF1 antibody  >2 publications with NOP56 and TCOF1
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