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NOP132 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS3903075

Nucleolar protein 132 (NOP132) was identified as a protein that interacts with the G protein, RRAG A (RAG A)/GTR1p, a member of the Ras-like small G protein family. NOP132 has been shown to interact mainly with proteins involved in ribosome biogenesis such as… More details

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Product Overview

Online Datasheet in PDF
Product Name
NOP132, Polyclonal Antibody
Full Product Name
Rabbit anti-NOP132 Antibody, Affinity Purified
Product Gene Name
Product Synonym Gene Name
NOL8
Product Synonym Names
bA62C3.4; C9orf34; NOP132; nucleolar protein 8; nucleolar protein Nop132; bA62C3.3; Nucleolar protein 8; nucleolar protein 8
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Purity/Purification
Antigen Affinity Purified
Concentration
1000ug/ml (lot specific)
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Molecular Weight
127,337 Da
OMIM
611534
3D Structure
ModBase 3D Structure for Q76FK4
Immunogen
Between 1117 and C-terminus
Conjugation
Unconjugated

Product Categories/Family for anti-NOP132 antibody

Applications Tested/Suitable for anti-NOP132 antibody
Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC)
Application Notes for anti-NOP132 antibody
WB: 1:2000-1:10,000
IP: 6ug/mg lysate
IHC: 1:1000-1:5000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

Immunohistochemistry (IHC) of anti-NOP132 antibody

Detection of human NOP132 by immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-NOP132 (Cat. No. MBS3903075 Lot1) used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB
Rabbit Anti-NOP132 Polyclonal Antibody – Cat# MBS3903075

Western Blot (WB) of anti-NOP132 antibody

Detection of human NOP132 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and HEK293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-NOP132 antibody MBS3903075 used for WB at 0.1 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. NOP132 was also immunoprecipitated by rabbit anti-NOP132 antibody MBS3903074, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 30 seconds (A and B).
Rabbit Anti-NOP132 Polyclonal Antibody, Western Blot (WB) – Cat# MBS3903075

Other Notes

Small volumes of anti-NOP132 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for NOP132 purchase

MBS3903075 is an antibody targeting NOP132. Also known as Rabbit anti-NOP132 Antibody, Affinity Purified. Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for NOP132. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
nucleolar protein 8 isoform a
NCBI Official Synonym Full Names
nucleolar protein 8
NCBI Official Symbol
NOL8
NCBI Official Synonym Symbols
NOP132; C9orf34; bA62C3.3; bA62C3.4
UniProt Protein Name
Nucleolar protein 8
UniProt Synonym Protein Names
Nucleolar protein Nop132
UniProt Gene Name
NOL8
UniProt Synonym Gene Names
C9orf34; NOP132
UniProt Entry Name
NOL8_HUMAN
NCBI Protein Information
nucleolar protein 8

NCBI Summary for NOP132

NOL8 binds Ras-related GTP-binding proteins (see MIM 608267) and plays a role in cell growth (Sekiguchi et al., 2004 [PubMed 14660641]).[supplied by OMIM, Mar 2008]

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Diseases associated with anti-NOP132 antibody

Organs/Tissues associated with anti-NOP132 antibody

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