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NOL12 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS3903085

Nucleolar protein 12 (NOL12) is a member of the RRP17 (ribosomal RNA-processing protein 17) family of proteins. NOL12 is inferred to be an RNA binding protein that binds 28S rRNA and localizes to the nucleolus. The function of NOL12 has not been characterized. More details

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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
NOL12, Polyclonal Antibody
Full Product Name
Rabbit anti-NOL12 Antibody, Affinity Purified
Product Gene Name
Product Synonym Names
dJ37E16.7; Nop25; nucleolar protein 12; Nucleolar protein 12; nucleolar protein 12
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human, Mouse
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Purity/Purification
Antigen Affinity Purified
Concentration
1000ug/ml (lot specific)
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Molecular Weight
24,663 Da
3D Structure
ModBase 3D Structure for Q9UGY1
Immunogen
Between 163 and C-terminus
Conjugation
Unconjugated

Product Categories/Family for anti-NOL12 antibody

Applications Tested/Suitable for anti-NOL12 antibody
Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC)
Application Notes for anti-NOL12 antibody
WB: 1:2000-1:10,000
IP: 6ug/mg lysate
IHC: 1:500-1:2000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

Immunohistochemistry (IHC) of anti-NOL12 antibody

Detection of human NOL12 by immunohistochemistry. Sample: FFPE section of human lung carcinoma. Antibody: Affinity purified rabbit anti-NOL12 (Cat. No. MBS3903085 Lot2) used at a dilution of 1:1,000 (1ug/ml) Detection: DAB
Rabbit Anti-NOL12 Polyclonal Antibody – Cat# MBS3903085

Immunoprecipitation (IP) of anti-NOL12 antibody

Detection of human NOL12 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-NOL12 antibody MBS3903085 (lot MBS3903085-2) used for IP at 3 ug per reaction. NOL12 was also immunoprecipitated by rabbit anti-NOL12 antibody MBS3903084. For blotting immunoprecipitated NOL12, MBS3903085 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.
Rabbit Anti-NOL12 Polyclonal Antibody, Immunoprecipitation (IP) – Cat# MBS3903085

Western Blot (WB) of anti-NOL12 antibody

Detection of human and mouse NOL12 by western mlot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-NOL12 antibody MBS3903085 (lot MBS3903085-2) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Rabbit Anti-NOL12 Polyclonal Antibody, Western Blot (WB) – Cat# MBS3903085

Other Notes

Small volumes of anti-NOL12 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for NOL12 purchase

MBS3903085 is an antibody targeting NOL12. Also known as Rabbit anti-NOL12 Antibody, Affinity Purified. Reactive in Human, Mouse. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for NOL12. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
nucleolar protein 12
NCBI Official Synonym Full Names
nucleolar protein 12
NCBI Official Symbol
NOL12
NCBI Official Synonym Symbols
Nop25; dJ37E16.7
UniProt Protein Name
Nucleolar protein 12
Protein Family
UniProt Gene Name
NOL12
UniProt Entry Name
NOL12_HUMAN
NCBI Protein Information
nucleolar protein 12

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Organs/Tissues associated with anti-NOL12 antibody

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