• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

ORC2 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS3903086

The origin recognition complex (ORC) is a highly conserved six subunits protein complex essential for the initiation of the DNA replication in eukaryotic cells. Studies in yeast demonstrated that ORC binds specifically to origins of replication and serves as… More details

$250 / 0.002 mg +1 FREE 8GB USB
Product Documentation
PDF
Sample Manual Insert View PDF
PDF
Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
PDF
Material Safety Data Sheet View PDF
PDF
Technical Datasheet View PDF
PDF
Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
ORC2, Polyclonal Antibody
Full Product Name
Rabbit anti-ORC2 Antibody, Affinity Purified
Product Gene Name
anti-ORC2 antibody
Product Synonym Names
ORC2L; origin recognition complex protein 2 homolog; origin recognition complex subunit 2; origin recognition complex, subunit 2 homolog; Origin recognition complex subunit 2; origin recognition complex subunit 2
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human
Form/Format
Liquid. Whole IgG. Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Purity/Purification
Antigen Affinity Purified
Concentration
200ug/ml (lot specific)
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Molecular Weight
65,972 Da
OMIM
601182
3D Structure
ModBase 3D Structure for Q13416
Immunogen
Between 1 and 50
Conjugation
Unconjugated

Product Categories/Family for anti-ORC2 antibody

Primary Antibodies
Applications Tested/Suitable for anti-ORC2 antibody
Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC)
Application Notes for anti-ORC2 antibody
WB: 1:2000-1:10,000
IP: 6ug/mg lysate
IHC: 1:200-1:1000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

Immunohistochemistry (IHC) of anti-ORC2 antibody

Detection of human ORC2 by immunohistochemistry. Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-ORC2 (Cat. No. MBS3903086) used at a dilution of 1:1,000 (0.2ug/ml) Detection: DAB
Rabbit Anti-ORC2 Polyclonal Antibody – Cat# MBS3903086

Western Blot (WB) of anti-ORC2 antibody

Detection of human ORC2 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and HEK293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-ORC2 antibody MBS3903086 used for WB at 0.04 ug/ml (A) and 0.4 ug/ml (B) and used for IP at 3 ug/mg lysate. ORC2 was also immunoprecipitated by rabbit anti-ORC2 antibody MBS3903087, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 10 seconds (B).
Rabbit Anti-ORC2 Polyclonal Antibody, Western Blot (WB) – Cat# MBS3903086

Other Notes

Small volumes of anti-ORC2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for ORC2 purchase

MBS3903086 is an antibody targeting ORC2. Also known as Rabbit anti-ORC2 Antibody, Affinity Purified. Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for ORC2. It may not necessarily be applicable to this product.

NCBI GI #
5453830
NCBI GeneID
4999
NCBI Accession #
NP_006181.1
UniProt Primary Accession #
Q13416
UniProt Secondary Accession #
Q13204; Q53TX5
UniProt Related Accession #
Q13416
GenBank Nucleotide #
NM_006190.4
NCBI Official Full Name
origin recognition complex subunit 2
NCBI Official Synonym Full Names
origin recognition complex, subunit 2
NCBI Official Symbol
ORC2
NCBI Official Synonym Symbols
ORC2L
UniProt Protein Name
Origin recognition complex subunit 2
Protein Family
ORC1-type DNA replication protein
UniProt Gene Name
ORC2
UniProt Synonym Gene Names
ORC2L
UniProt Entry Name
ORC2_HUMAN
NCBI Protein Information
origin recognition complex subunit 2; origin recognition complex protein 2 homolog; origin recognition complex, subunit 2 homolog

NCBI Summary for ORC2

The origin recognition complex (ORC) is a highly conserved six subunits protein complex essential for the initiation of the DNA replication in eukaryotic cells. Studies in yeast demonstrated that ORC binds specifically to origins of replication and serves as a platform for the assembly of additional initiation factors such as Cdc6 and Mcm proteins. The protein encoded by this gene is a subunit of the ORC complex. This protein forms a core complex with ORC3, -4, and -5. It also interacts with CDC45 and MCM10, which are proteins known to be important for the initiation of DNA replication. This protein has been demonstrated to specifically associate with the origin of replication of Epstein-Barr virus in human cells, and is thought to be required for DNA replication from viral origin of replication. Alternatively spliced transcript variants have been found, one of which is a nonsense-mediated mRNA decay candidate. [provided by RefSeq, Oct 2010]
Show more

Research Articles on ORC2

1. Protein phosphatase 1 dephosphorylates Orc2 to promote the binding of ORC to chromatin and replication origins for the subsequent round of the cell cycle.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-ORC2 antibody

 Reference Product  PubMed Publications
 CDC6 antibody  >30 publications with ORC2 and CDC6
 MCM3 antibody  >18 publications with ORC2 and MCM3
 MCM2 antibody  >17 publications with ORC2 and MCM2
 CDC45 antibody  >11 publications with ORC2 and CDC45
 MCM10 antibody  >6 publications with ORC2 and MCM10
 ORC1L antibody  >1 publications with ORC2 and ORC1L
 ORC3L antibody  >1 publications with ORC2 and ORC3L

Pathways associated with anti-ORC2 antibody

 Products by Pathway  Pathway Diagram
 Activation Of ATR In Response To Replication Stress Pathway antibodies  Activation Of ATR In Response To Replication Stress Pathway Diagram
 Activation Of The Pre-replicative Complex Pathway antibodies  Activation Of The Pre-replicative Complex Pathway Diagram
 Assembly Of The ORC Complex At The Origin Of Replication Pathway antibodies  Assembly Of The ORC Complex At The Origin Of Replication Pathway Diagram
 Assembly Of The Pre-replicative Complex Pathway antibodies  Assembly Of The Pre-replicative Complex Pathway Diagram
 Association Of Licensing Factors With The Pre-replicative Complex Pathway antibodies  Association Of Licensing Factors With The Pre-replicative Complex Pathway Diagram
 CDC6 Association With The ORC:origin Complex Pathway antibodies  CDC6 Association With The ORC:origin Complex Pathway Diagram
 CDT1 Association With The CDC6:ORC:origin Complex Pathway antibodies  CDT1 Association With The CDC6:ORC:origin Complex Pathway Diagram
 Cell Cycle Pathway antibodies  Cell Cycle Pathway Diagram
 Cell Cycle Checkpoints Pathway antibodies  Cell Cycle Checkpoints Pathway Diagram
 Cell Cycle, Mitotic Pathway antibodies  Cell Cycle, Mitotic Pathway Diagram

Diseases associated with anti-ORC2 antibody

 Disease Name  Pubmed Publications
 Cell Transformation, Neoplastic Antibodies  >3 publications with ORC2 and Cell Transformation, Neoplastic
 Memory Disorders Antibodies  >1 publications with ORC2 and Memory Disorders
 Neurobehavioral Manifestations Antibodies  >1 publications with ORC2 and Neurobehavioral Manifestations
 Adenocarcinoma Antibodies  >1 publications with ORC2 and Adenocarcinoma
 Neoplasms, Experimental Antibodies  >1 publications with ORC2 and Neoplasms, Experimental
 Necrosis Antibodies  >1 publications with ORC2 and Necrosis

Organs/Tissues associated with anti-ORC2 antibody

 Organ/Tissue Name  Pubmed Publications
 Embryonic Tissue Antibodies  >8 publications with ORC2 and Embryonic Tissue
 Salivary Gland Antibodies  >3 publications with ORC2 and Salivary Gland
 Eye Antibodies  >3 publications with ORC2 and Eye
 Blood Antibodies  >2 publications with ORC2 and Blood
 Kidney Antibodies  >2 publications with ORC2 and Kidney
 Brain Antibodies  >2 publications with ORC2 and Brain
 Testis Antibodies  >2 publications with ORC2 and Testis
 Mouth Antibodies  >1 publications with ORC2 and Mouth
 Lung Antibodies  >1 publications with ORC2 and Lung
 Liver Antibodies  >1 publications with ORC2 and Liver
Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000