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MSK2 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS3903098

MSK1 is a serine/threonine kinase required for the mitogen or stress-induced phosphorylation of the transcription factors CREB (cAMP response element-binding protein) and ATF1 (activating transcription factor-1). MSK1 Directly represses transcription via… More details

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Testing Data View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
MSK2, Polyclonal Antibody
Full Product Name
Rabbit anti-MSK2 Antibody, Affinity Purified
Product Gene Name
anti-MSK2 antibody
Product Synonym Gene Name
RPS6KA4
Product Synonym Names
MSK2; RSK-B; RSKB; S6K-alpha-4; ribosomal protein S6 kinase, 90kDa, polypeptide 4; ribosomal protein S6 kinase alpha-4; nuclear mitogen- and stress-activated protein kinase 2; mitogen- and stress-activated protein kinase 2; 90 kDa ribosomal protein S6 kinase 4; ribosomal protein kinase B; Ribosomal protein S6 kinase alpha-4; ribosomal protein S6 kinase A4
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human, Mouse
Form/Format
Liquid. Whole IgG. Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Purity/Purification
Antigen Affinity Purified
Concentration
200ug/ml (lot specific)
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
OMIM
603606
3D Structure
ModBase 3D Structure for O75676
Immunogen
Between 722 and C-terminus
Conjugation
Unconjugated

Product Categories/Family for anti-MSK2 antibody

Primary Antibodies
Applications Tested/Suitable for anti-MSK2 antibody
Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC)
Application Notes for anti-MSK2 antibody
WB: 1:2000-1:10,000
IP: 6ug/mg lysate
IHC: 1:100-1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

Immunohistochemistry (IHC) of anti-MSK2 antibody

Detection of human MSK2 by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-MSK2 (Cat. No. MBS3903098) used at a dilution of 1:200 (1ug/ml) Detection: DAB
Rabbit Anti-MSK2 Polyclonal Antibody – Cat# MBS3903098

Western Blot (WB) of anti-MSK2 antibody

Detection of human and mouse MSK2 by western blot (h&m) and immunoprecipitation (h). Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), HEK293T (T; 50 ug), and mouse NIH 3T3 (M; 50 ug) cells. Antibodies: Affinity purified rabbit anti-MSK2 antibody MBS3903098 used for WB at 0.04 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. MSK2 was also immunoprecipitated by rabbit anti-MSK2 antibody BL9977, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 30 seconds (A and B).
Rabbit Anti-MSK2 Polyclonal Antibody, Western Blot (WB) – Cat# MBS3903098

Other Notes

Small volumes of anti-MSK2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for MSK2 purchase

MBS3903098 is an antibody targeting MSK2. Also known as Rabbit anti-MSK2 Antibody, Affinity Purified. Reactive in Human, Mouse. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for MSK2. It may not necessarily be applicable to this product.

NCBI GeneID
8986
NCBI Accession #
NP_003933.1
UniProt Primary Accession #
O75676
UniProt Protein Name
Ribosomal protein S6 kinase alpha-4
UniProt Synonym Protein Names
90 kDa ribosomal protein S6 kinase 4; Nuclear mitogen- and stress-activated protein kinase 2; Ribosomal protein kinase B
UniProt Gene Name
RPS6KA4
UniProt Synonym Gene Names
MSK2; S6K-alpha-4; RSKB
UniProt Entry Name
KS6A4_HUMAN

UniProt Comments for MSK2

MSK2: an AGC kinase of the RSK family. Has two kinase domains connected by a regulatory linker region. Phosphorylates H3 and HMG-14 in response to growth factors and cellular stress.

Protein type: EC 2.7.11.1; Protein kinase, AGC; Kinase, protein; Protein kinase, Ser/Thr (non-receptor); AGC group; RSK family; MSK subfamily

Chromosomal Location of Human Ortholog: 11q11-q13

Cellular Component: nucleoplasm; cytoplasm; nucleus

Molecular Function: protein serine/threonine kinase activity; protein binding; mitogen-activated protein kinase p38 binding; magnesium ion binding; ribosomal protein S6 kinase activity; ATP binding

Biological Process: axon guidance; regulation of transcription, DNA-dependent; positive regulation of histone phosphorylation; histone phosphorylation; negative regulation of cytokine production; activation of CREB transcription factor; positive regulation of histone acetylation; positive regulation of transcription from RNA polymerase II promoter; inflammatory response; protein amino acid phosphorylation; activation of NF-kappaB transcription factor
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
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