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SPF30 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS3903176

SPF30 (splicing factor 30) is encoded by the SMNDC1 (survival motor neuron domain containing 1) gene and is a paralog of the SMN1 (survival motor neuron) protein. SPF30 has been identified as an essential component of the splicesome complex. More details

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Product Overview

Online Datasheet in PDF
Product Name
SPF30, Polyclonal Antibody
Full Product Name
Rabbit anti-SPF30 Antibody, Affinity Purified
Product Gene Name
Product Synonym Gene Name
SMNDC1
Product Synonym Names
survival motor neuron domain-containing protein 1; SMN-related protein; tudor domain containing 16C; TDRD16C; survival of motor neuron-related-splicing factor 30; SPF30; 30 kDa splicing factor SMNrp; SMNR; splicing factor 30, survival of motor neuron-related; Survival of motor neuron-related-splicing factor 30; survival motor neuron domain containing 1
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human, Mouse
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Purity/Purification
Antigen Affinity Purified
Concentration
1000ug/ml (lot specific)
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Molecular Weight
26,711 Da
OMIM
603519
3D Structure
ModBase 3D Structure for O75940
Predicted Reactivity
Rat, X. tropicalis, Bovine, Orangutan
Immunogen
Between 188 and C-terminus
Conjugation
Unconjugated

Product Categories/Family for anti-SPF30 antibody

Applications Tested/Suitable for anti-SPF30 antibody
Western Blot (WB), Immunoprecipitation (IP)
Application Notes for anti-SPF30 antibody
WB: 1:2000-1:10,000
IP: 6ug/mg lysate

Western Blot (WB) of anti-SPF30 antibody

Detection of human and mouse SPF30 by western blot (h&m) and immunoprecipitation (h). Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), HEK293T (T; 50 ug), and mouse NIH 3T3 (M; 50 ug) cells. Antibodies: Affinity purified rabbit anti-SPF30 antibody MBS3903176 used for WB at 0.1 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. SPF30 was also immunoprecipitated by rabbit anti-SPF30 antibody MBS3903175, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 10 seconds (B).
Rabbit Anti-SPF30 Polyclonal Antibody – Cat# MBS3903176

Other Notes

Small volumes of anti-SPF30 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for SPF30 purchase

MBS3903176 is an antibody targeting SPF30. Also known as Rabbit anti-SPF30 Antibody, Affinity Purified. Reactive in Human, Mouse. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for SPF30. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
survival of motor neuron-related-splicing factor 30
NCBI Official Synonym Full Names
survival motor neuron domain containing 1
NCBI Official Symbol
SMNDC1
NCBI Official Synonym Symbols
SMNR; SPF30; TDRD16C
UniProt Protein Name
Survival of motor neuron-related-splicing factor 30
UniProt Synonym Protein Names
30 kDa splicing factor SMNrp; SMN-related protein; Survival motor neuron domain-containing protein 1
Protein Family
UniProt Gene Name
SMNDC1
UniProt Synonym Gene Names
SMNR; SPF30
UniProt Entry Name
SPF30_HUMAN
NCBI Protein Information
survival of motor neuron-related-splicing factor 30; 30 kDa splicing factor SMNrp; SMN-related protein; splicing factor 30, survival of motor neuron-related; survival motor neuron domain-containing protein 1; tudor domain containing 16C

NCBI Summary for SPF30

This gene is a paralog of SMN1 gene, which encodes the survival motor neuron protein, mutations in which are cause of autosomal recessive proximal spinal muscular atrophy. The protein encoded by this gene is a nuclear protein that has been identified as a constituent of the spliceosome complex. This gene is differentially expressed, with abundant levels in skeletal muscle, and may share similar cellular function as the SMN1 gene. [provided by RefSeq, Jul 2008]
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UniProt Comments for SPF30

SPF30: Necessary for spliceosome assembly. Overexpression causes apoptosis. Belongs to the SMN family.

Protein type: Spliceosome; RNA processing; RNA splicing; RNA-binding

Chromosomal Location of Human Ortholog: 10q23

Cellular Component: nucleoplasm; spliceosome; Cajal body; intermediate filament cytoskeleton; cytoplasm; nuclear speck; nucleus

Molecular Function: protein binding

Biological Process: RNA splicing, via transesterification reactions; apoptosis; RNA splicing; spliceosome assembly; mRNA processing
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-SPF30 antibody

Pathways associated with anti-SPF30 antibody

Diseases associated with anti-SPF30 antibody

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