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SmcHD1 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS3903210

SmcHD1 (structural maintenance of chromosomes flexible hinge domain-containing protein 1) has been shown to play a role in the maintenance of X-inactivation and the hypermethylation of CpG islands associated with inactive X. More details

$250 / 0.002 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
SmcHD1, Polyclonal Antibody
Full Product Name
Rabbit anti-SmcHD1 Antibody, Affinity Purified
Product Gene Name
Product Synonym Names
BAMS; structural maintenance of chromosomes flexible hinge domain-containing protein 1; FSHD2; SMC hinge domain-containing protein 1; Structural maintenance of chromosomes flexible hinge domain-containing protein 1; structural maintenance of chromosomes flexible hinge domain containing 1
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human
Form/Format
Liquid. Whole IgG. Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Purity/Purification
Antigen Affinity Purified
Concentration
200ug/ml (lot specific)
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Molecular Weight
226,374 Da
Chromosome Location
Chromosome: 18; NC_000018.9 (2655886..2805015). Location: 18p11.32
OMIM
158901
3D Structure
ModBase 3D Structure for A6NHR9
Immunogen
Between 1955 and C-terminus
Conjugation
Unconjugated

Product Categories/Family for anti-SmcHD1 antibody

Applications Tested/Suitable for anti-SmcHD1 antibody
Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC)
Application Notes for anti-SmcHD1 antibody
WB: 1:2000-1:10,000 (3-8% Tris-acetate gel recommended)
IP: 6ug/mg lysate
IHC: 1:100-1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

Immunohistochemistry (IHC) of anti-SmcHD1 antibody

Detection of human SmcHD1 by immunohistochemistry. Sample: FFPE section of human non-small cell lung cancer (left) and testicular seminoma (right). Antibody: Affinity purified rabbit anti-SmcHD1 (Cat. No. MBS3903210 Lot1) used at a dilution of 1:200 (1ug/ml). Detection: Vector Laboratories ImmPACT NovaRED Peroxidase Substrate
Rabbit Anti-SmcHD1 Polyclonal Antibody – Cat# MBS3903210

Western Blot (WB) of anti-SmcHD1 antibody

Detection of human SmcHD1 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and HEK293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-SmcHD1 antibody MBS3903210 used for WB at 0.04 ug/ml (A) and 0.4 ug/ml (B) and used for IP at 3 ug/mg lysate. SmcHD1 was also immunoprecipitated by rabbit anti-SmcHD1 antibody MBS3903209, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 3 seconds (B).
Rabbit Anti-SmcHD1 Polyclonal Antibody, Western Blot (WB) – Cat# MBS3903210

Other Notes

Small volumes of anti-SmcHD1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for SmcHD1 purchase

MBS3903210 is an antibody targeting SmcHD1. Also known as Rabbit anti-SmcHD1 Antibody, Affinity Purified. Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for SmcHD1. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
structural maintenance of chromosomes flexible hinge domain-containing protein 1
NCBI Official Synonym Full Names
structural maintenance of chromosomes flexible hinge domain containing 1
NCBI Official Symbol
SMCHD1
UniProt Protein Name
Structural maintenance of chromosomes flexible hinge domain-containing protein 1
UniProt Synonym Protein Names
SMC hinge domain-containing protein 1
UniProt Gene Name
SMCHD1
UniProt Synonym Gene Names
KIAA0650
UniProt Entry Name
SMHD1_HUMAN
NCBI Protein Information
structural maintenance of chromosomes flexible hinge domain-containing protein 1; SMC hinge domain-containing protein 1

NCBI Summary for SmcHD1

This gene encodes a protein which contains a hinge region domain found in members of the SMC (structural maintenance of chromosomes) protein family. [provided by RefSeq, Dec 2011]

UniProt Comments for SmcHD1

SMCHD1: Required for maintenance of X inactivation in females and hypermethylation of CpG islands associated with inactive X. Involved in a pathway that mediates the methylation of a subset of CpG islands slowly and requires the de novo methyltransferase DNMT3B. 3 isoforms of the human protein are produced by alternative splicing.

Chromosomal Location of Human Ortholog: 18p11.32

Cellular Component: Barr body

Molecular Function: ATP binding

Biological Process: chromosome organization and biogenesis

Disease: Facioscapulohumeral Muscular Dystrophy 2
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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