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MPP8 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS3903357

M-phase phosphoprotein 8 (MPP8) is a methyl-H3K9-binding protein and has been shown to direct DNA mehthylation. More details

$250 / 0.01 mg +1 FREE 8GB USB
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
MPP8, Polyclonal Antibody
Full Product Name
Rabbit anti-MPP8 Antibody, Affinity Purified
Product Gene Name
Product Synonym Gene Name
MPHOSPH8
Product Synonym Names
M-phase phosphoprotein, mpp8; TWA3; mpp8; M-phase phosphoprotein 8; HSMPP8; two hybrid-associated protein 3 with RanBPM; M-phase phosphoprotein, mpp; M-phase phosphoprotein 8; M-phase phosphoprotein 8
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Purity/Purification
Antigen Affinity Purified
Concentration
1000ug/ml (lot specific)
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Molecular Weight
97,182 Da
Chromosome Location
Chromosome: 13; NC_000013.11 (19633647..19673459). Location: 13q12.11
3D Structure
ModBase 3D Structure for Q99549
Immunogen
Between 1 and 50
Conjugation
Unconjugated

Product Categories/Family for anti-MPP8 antibody

Applications Tested/Suitable for anti-MPP8 antibody
Western Blot (WB), Immunoprecipitation (IP)
Application Notes for anti-MPP8 antibody
WB: 1:2000-1:10,000
IP: 6ug/mg lysate

Western Blot (WB) of anti-MPP8 antibody

Detection of human MPP8 by western blot and immunoprecipitation. Samples: Whole cell lysate (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-MPP8 antibody MBS3903357 used for WB at 0.1 ug/ml (A) and 1 ug/ml (B) and used for IP at 6 ug/mg lysate. MPP8 was also immunoprecipitated by rabbit anti-MPP8 antibody MBS3903358, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 3 seconds (B).
Rabbit Anti-MPP8 Polyclonal Antibody – Cat# MBS3903357

Other Notes

Small volumes of anti-MPP8 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for MPP8 purchase

MBS3903357 is an antibody targeting MPP8. Also known as Rabbit anti-MPP8 Antibody, Affinity Purified. Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for MPP8. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
M-phase phosphoprotein 8
NCBI Official Synonym Full Names
M-phase phosphoprotein 8
NCBI Official Symbol
MPHOSPH8
NCBI Official Synonym Symbols
TWA3; mpp8; HSMPP8; RP11-523H24.1
UniProt Protein Name
M-phase phosphoprotein 8
UniProt Synonym Protein Names
Two hybrid-associated protein 3 with RanBPM
UniProt Gene Name
MPHOSPH8
UniProt Synonym Gene Names
Twa31 PublicationManual assertion based on opinion iniRef.7
NCBI Protein Information
M-phase phosphoprotein 8; M-phase phosphoprotein, mpp; M-phase phosphoprotein, mpp8; two hybrid-associated protein 3 with RanBPM

UniProt Comments for MPP8

Heterochromatin component that specifically recognizes and binds methylated 'Lys-9' of histone H3 (H3K9me) and promotes recruitment of proteins that mediate epigenetic repression (PubMed:20871592, PubMed:26022416). Mediates recruitment of the HUSH complex to H3K9me3 sites: the HUSH complex is recruited to genomic loci rich in H3K9me3 and is probably required to maintain transcriptional silencing by promoting recruitment of SETDB1, a histone methyltransferase that mediates further deposition of H3K9me3 (PubMed:26022416). Binds H3K9me and promotes DNA methylation by recruiting DNMT3A to target CpG sites; these can be situated within the coding region of the gene (PubMed:20871592). Mediates down-regulation of CDH1 expression (PubMed:20871592).
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Organs/Tissues associated with anti-MPP8 antibody

 Organ/Tissue Name  Pubmed Publications
 Thyroid Antibodies  >1 publications with MPP8 and Thyroid
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