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GR Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS3903757

The glucocorticoid receptor (GR) functions as both a transcription factor that binds to glucocorticoid response elements in the promoters of glucocorticoid responsive genes to activate their transcription, and as a regulator of other transcription factors.… More details

$250 / 0.002 mg +1 FREE 8GB USB
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
GR, Polyclonal Antibody
Full Product Name
Rabbit anti-GR Antibody, Affinity Purified
Product Gene Name
anti-GR antibody
Product Synonym Gene Name
NR3C1
Product Synonym Names
glucocorticoid nuclear receptor variant 1; nuclear receptor subfamily 3, group C, member 1 (glucocorticoid receptor); Nuclear receptor subfamily 3 group C member 1; GRL; glucocorticoid receptor; GCRST; GCR; GCCR; GR; Glucocorticoid receptor; nuclear receptor subfamily 3 group C member 1
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human
Form/Format
Liquid. Whole IgG. Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Purity/Purification
Antigen Affinity Purified
Concentration
200ug/ml (lot specific)
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
OMIM
138040
3D Structure
ModBase 3D Structure for P04150
Predicted Reactivity
Sheep
Immunogen
Between 150 and 200
Conjugation
Unconjugated

Product Categories/Family for anti-GR antibody

Primary Antibodies
Applications Tested/Suitable for anti-GR antibody
Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), Chromatin Immunoprecipitation-Seq (ChIP-Seq)
Application Notes for anti-GR antibody
WB: 1:2000-1:10,000
IP: 6ug/mg lysate
IHC: 1:500-1:2000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
ChIP-Seq: 4ug/30 ug chromatin

Chromatin Immunoprecipitation (ChIP) of anti-GR antibody

Localization of GR Binding Sites by ChIP-sequencing. Chromatin from prednisolone treated THP-1 cells was immunoprecipitated with anti-GR antibody MBS3903757 and analyzed by DNA sequencing. The figure illustrates the peak distribution of GR binding within a 250 Kb region of chromosome 6 as detected using anti-GR MBS3903757. ChIP-seq validation performed by Active Motif, Carlsbad, CA.
Rabbit Anti-GR Polyclonal Antibody – Cat# MBS3903757

Immunohistochemistry (IHC) of anti-GR antibody

Detection of human GR by immunohistochemistry. Sample: FFPE section of human lung carcinoma. Antibody: Affinity purified rabbit anti-GR (Cat. No. MBS3903757 Lot1) used at a dilution of 1:200 (1ug/ml). Detection: DAB
Rabbit Anti-GR Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS3903757

Western Blot (WB) of anti-GR antibody

Detection of human GR by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and HEK293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-GR antibody MBS3903757 used for WB at 0.04 ug/ml (A) and 0.4 ug/ml (B) and used for IP at 6 ug/mg lysate. GR was also immunoprecipitated by rabbit anti-GR antibody MBS3903756, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 1 second (B).
Rabbit Anti-GR Polyclonal Antibody, Western Blot (WB) – Cat# MBS3903757

Other Notes

Small volumes of anti-GR antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for GR purchase

MBS3903757 is an antibody targeting GR. Also known as Rabbit anti-GR Antibody, Affinity Purified. Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for GR. It may not necessarily be applicable to this product.

NCBI GeneID
2908
NCBI Accession #
NP_032199
UniProt Primary Accession #
P04150
UniProt Protein Name
Glucocorticoid receptor
UniProt Synonym Protein Names
Nuclear receptor subfamily 3 group C member 1
Protein Family
Grahamin
UniProt Gene Name
NR3C1
UniProt Synonym Gene Names
GRL; GR
UniProt Entry Name
GCR_HUMAN

UniProt Comments for GR

GR: transcription factor of the nuclear receptor family. Receptor for glucocorticoids (GC). Binds to glucocorticoid response elements (GRE) and modulates other transcription factors. Affects inflammatory responses, cellular proliferation and differentiation in target tissues. Three alternatively spliced isoforms have been described.

Protein type: Transcription factor; DNA-binding; Mitochondrial; Nuclear receptor

Chromosomal Location of Human Ortholog: 5q31.3

Cellular Component: nucleoplasm; membrane; mitochondrial matrix; cytoplasm; cytosol; nucleus

Molecular Function: protein dimerization activity; glucocorticoid receptor activity; protein binding; zinc ion binding; transcription factor activity; steroid binding

Biological Process: transcription from RNA polymerase II promoter; transcription initiation from RNA polymerase II promoter; transcription, DNA-dependent; maternal behavior; adrenal gland development; regulation of glucocorticoid biosynthetic process; chromatin modification; signal transduction; glucocorticoid metabolic process; glucocorticoid mediated signaling; regulation of transcription, DNA-dependent; positive regulation of neuron apoptosis; glucocorticoid receptor signaling pathway; gene expression; positive regulation of transcription from RNA polymerase II promoter; regulation of gluconeogenesis

Disease: Glucocorticoid Resistance, Generalized
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
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