Rabbit Anti-ETO Polyclonal Antibody – Cat# MBS3903775

Detection of human ETO by western blot and immunoprecipitation. Samples: Whole cell lysate from HEK293T (15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), Jurkat (J; 50 ug) and HeLa (H; 50 ug) cells. Antibodies: Affinity purified rabbit anti-ETO antibody MBS3903775 used for WB at 0.4 ug/ml (A) and 1 ug/ml (B) and used for IP at 6 ug/mg lysate. ETO was also immunoprecipitated by rabbit anti-ETO antibody BL12518, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 30 seconds (A and B).

ETO Antibody – Rabbit Polyclonal

Name: ETO Antibody; Polyclonal; Rabbit; Human-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS3903775
Price: 250 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS3903775

ETO (eight twenty-one protein) is also known as MTG8 (myeloid translocation gene on 8q22) and was identified as part of an 8:21 chromosomal breakpoint in leukemia cells. ETO is encoded by the RUNX1T1 (runt-related transcription factor; translocated to, 1… More details

$250 / 0.01 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

ETO, Polyclonal Antibody

Full Product Name

Rabbit anti-ETO Antibody, Affinity Purified

Product Gene Name

anti-ETO antibody

Product Synonym Gene Name

RUNX1T1

Product Synonym Names

MTG8; CDR; zinc finger MYND domain-containing protein 2; runt related transcription factor 1; translocated to, 1 (cyclin D related); Protein MTG8; Protein ETO; protein CBFA2T1; myeloid translocation gene on 8q22; ZMYND2; eight twenty one protein; Cyclin-D-related protein; core-binding factor, runt domain, alpha subunit 2; translocated to, 1; CBFA2T1; AML1T1; AML1-MTG8; acute myelogenous leukemia 1 translocation 1, cyclin-D related; cyclin D-related; ETO; Protein CBFA2T1; RUNX1 translocation partner 1

Clonality

Polyclonal

Host

Rabbit

Species Reactivity

Human

Form/Format

Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide

Purity/Purification

Antigen Affinity Purified

Concentration

1000ug/ml (lot specific)

Preparation and Storage

Store at 2-8 degree C
Shelf Life: 1 year from date of receipt

OMIM

phenotype 133435

3D Structure

ModBase 3D Structure for Q06455

Predicted Reactivity

Mouse

Immunogen

Between 554 and C-terminus

Conjugation

Unconjugated

Product Categories/Family for anti-ETO antibody

Primary Antibodies

Applications Tested/Suitable for anti-ETO antibody

Western Blot (WB), Immunoprecipitation (IP)

Application Notes for anti-ETO antibody

WB: 1:500-1:2500
IP: 6ug/mg lysate

Other Notes

Small volumes of anti-ETO antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for ETO purchase

MBS3903775 is an antibody targeting ETO. Also known as Rabbit anti-ETO Antibody, Affinity Purified. Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for ETO. It may not necessarily be applicable to this product.

NCBI GeneID

862

NCBI Accession #

NP_783553.1

UniProt Primary Accession #

Q06455

UniProt Protein Name

Protein CBFA2T1

UniProt Synonym Protein Names

Cyclin-D-related protein; Eight twenty one protein; Protein ETO; Protein MTG8; Zinc finger MYND domain-containing protein 2

Protein Family

ETO1-like protein

UniProt Gene Name

RUNX1T1

UniProt Synonym Gene Names

AML1T1; CBFA2T1; CDR; ETO; MTG8; ZMYND2

UniProt Entry Name

MTG8_HUMAN

UniProt Comments for ETO

RUNX1T1: Transcription regulator that excerts its function by binding to histone deacetylases and transcription factors. Can repress transactivation mediated by TCF12. Homotetramer. Heterotetramer with CBFA2T2 and CBFA2T3. Interacts with TCF12, SIN3A, HDAC1, HDAC2, HDAC3, NCOR1 and NCOR2. Interacts with ATN1 (via its N-terminus); the interaction enhances the transcriptional repression. Most abundantly expressed in brain. Lower levels in lung, heart, testis and ovary. Belongs to the CBFA2T family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Transcription regulation; Oncoprotein

Chromosomal Location of Human Ortholog: 8q22

Cellular Component: nucleoplasm; nuclear matrix; mitochondrion; cytoplasm

Molecular Function: identical protein binding; protein binding; DNA binding; metal ion binding; transcription factor activity; transcription corepressor activity

Biological Process: generation of precursor metabolites and energy; transcription, DNA-dependent; negative regulation of transcription, DNA-dependent

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.