Rabbit Anti-PI4KB Polyclonal Antibody – Cat# MBS3903982

Detection of human PI4KB by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), HEK293T (T; 50 ug) and Jurkat (J; 50 ug) cells. Antibodies: Affinity purified rabbit anti-PI4KB antibody MBS3903982 used for WB at 0.1 ug/ml (A) and 1 ug/ml (B) and used for IP at 6 ug/mg lysate. PI4KB was also immunoprecipitated by rabbit anti-PI4KB antibody MBS3903983, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 10 seconds (A) and 3 seconds (B).

PI4KB Antibody – Rabbit Polyclonal

Name: PI4KB Antibody; Polyclonal; Rabbit; Human-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS3903982
Price: 250 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS3903982

Phosphatidylinositol 4-kinase beta (PI4KB) phosphorylates phosphatidylinositol (PI) in the first committed step in the production of the second messenger inositol-1,4,5,-trisphosphate (PIP). PI4KB may regulate Golgi disintegration/reorganization during… More details

$250 / 0.01 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

PI4KB, Polyclonal Antibody

Full Product Name

Rabbit anti-PI4KB Antibody, Affinity Purified

Product Gene Name

anti-PI4KB antibody

Product Synonym Names

PI4K-BETA; NPIK; PtdIns 4-kinase beta; type III phosphatidylinositol 4-kinase beta; PIK4CB; PI4KIIIBETA; PI4K92; phosphatidylinositol 4-kinase, wortmannin-sensitive; phosphatidylinositol 4-kinase beta; phosphatidylinositol 4-kinase, catalytic, beta; PI4KBETA; Phosphatidylinositol 4-kinase beta; phosphatidylinositol 4-kinase beta

Clonality

Polyclonal

Host

Rabbit

Species Reactivity

Human

Form/Format

Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide

Purity/Purification

Antigen Affinity Purified

Concentration

1000ug/ml (lot specific)

Preparation and Storage

Store at 2-8 degree C
Shelf Life: 1 year from date of receipt

OMIM

602758

3D Structure

ModBase 3D Structure for Q9UBF8

Immunogen

Between 1 and 50

Conjugation

Unconjugated

Product Categories/Family for anti-PI4KB antibody

Primary Antibodies

Applications Tested/Suitable for anti-PI4KB antibody

Western Blot (WB), Immunoprecipitation (IP)

Application Notes for anti-PI4KB antibody

WB: 1:2000-1:10,000
IP: 6ug/mg lysate

Other Notes

Small volumes of anti-PI4KB antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for PI4KB purchase

MBS3903982 is an antibody targeting PI4KB. Also known as Rabbit anti-PI4KB Antibody, Affinity Purified. Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for PI4KB. It may not necessarily be applicable to this product.

NCBI GeneID

5298

NCBI Accession #

CAA09495.1

UniProt Primary Accession #

Q9UBF8

UniProt Protein Name

Phosphatidylinositol 4-kinase beta

UniProt Synonym Protein Names

NPIK; PI4K92

Protein Family

Phosphatidylinositol 4-kinase

UniProt Gene Name

PI4KB

UniProt Synonym Gene Names

PIK4CB; PI4K-beta; PI4Kbeta

UniProt Entry Name

PI4KB_HUMAN

UniProt Comments for PI4KB

PIK4CB: a member of the PI3/PI4 kinase family. Phosphorylates phosphatidylinositol (PI) in the first committed step in the production of the second messenger inositol- 1,4,5,-trisphosphate. Found in the outer membrane of mitochondria and membranes of the rough endoplasmic reticulum. Recruited to the Golgi complex by the small GTPase ARF to stimulate the synthesis of phosphatidylinositol 4,5-biphosphate (PIP2) on the Golgi complex. May regulate Golgi disintegration/reorganization during mitosis, possibly via its phosphorylation. Three splice-variant isoforms have been described.

Protein type: Kinase, lipid; EC 2.7.1.67; Motility/polarity/chemotaxis; Carbohydrate Metabolism - inositol phosphate

Chromosomal Location of Human Ortholog: 1q21

Cellular Component: Golgi membrane; mitochondrial outer membrane; perinuclear region of cytoplasm; cytoplasm; plasma membrane; rough endoplasmic reticulum membrane; cytosol; endosome

Molecular Function: 1-phosphatidylinositol 4-kinase activity; protein binding; ATP binding

Biological Process: receptor-mediated endocytosis; phosphoinositide-mediated signaling; phosphoinositide phosphorylation; phospholipid metabolic process; phosphatidylinositol biosynthetic process; signal transduction

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.