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SMC1 Antibody – Goat Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS3904078

Structural maintenance of chromosomes (SMC) proteins are part of the cohesin and condensin multisubunit complexes that play a critical role in chromosome organization, segregation, gene regulation, and DNA repair. The core cohesin complex includes a… More details

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Product Overview

Online Datasheet in PDF
Product Name
SMC1, Polyclonal Antibody
Full Product Name
Goat anti-SMC1 Antibody, Affinity Purified
Product Gene Name
anti-SMC1 antibody
Product Synonym Gene Name
SMC1A
Product Synonym Names
SMC protein 1A; SMC-1A; structural maintenance of chromosomes protein 1A; SMCB; SMC1L1; SMC-1-alpha; SMC1alpha; SMC1; segregation of mitotic chromosomes 1; SB1.8; epididymis secretory sperm binding protein; DXS423E; CDLS2; SMC1 (structural maintenance of chromosomes 1, yeast)-like 1; Structural maintenance of chromosomes protein 1A; structural maintenance of chromosomes 1A
Clonality
Polyclonal
Host
Goat
Species Reactivity
Human, Mouse
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Purity/Purification
Antigen Affinity Purified
Concentration
1000ug/ml (lot specific)
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Molecular Weight
143,233 Da
Chromosome Location
Chromosome: X; NC_000023.10 (53401070..53449618, complement). Location: Xp11.22-p11.21
OMIM
300040
3D Structure
ModBase 3D Structure for Q14683
Predicted Reactivity
Rat, Bovine
Immunogen
Between 1183 and C-terminus
Conjugation
Unconjugated

Product Categories/Family for anti-SMC1 antibody

Primary Antibodies
Applications Tested/Suitable for anti-SMC1 antibody
Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC)
Application Notes for anti-SMC1 antibody
WB: 1:2000-1:10,000
IP: 6ug/mg lysate
IHC: 1:500-1:2000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

Immunohistochemistry (IHC) of anti-SMC1 antibody

Detection of human SMC1 by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified goat anti-SMC1 (Cat. No. MBS3904078 Lot1) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB
Goat Anti-SMC1 Polyclonal Antibody – Cat# MBS3904078

Immunohistochemistry (IHC) of anti-SMC1 antibody

Detection of mouse SMC1 by immunohistochemistry. Sample: FFPE section of mouse renal cell carcinoma. Antibody: Affinity purified goat anti-SMC1 (Cat. No. MBS3904078 Lot1) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB
Goat Anti-SMC1 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS3904078

Immunoprecipitation (IP) of anti-SMC1 antibody

Detection of human SMC1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified goat anti-SMC1 antibody MBS3904078 (lot MBS3904078-1) used for IP at 6 ug/mg lysate. SMC1 was also immunoprecipitated by rabbit anti-SMC1 antibody MBS3900729. For blotting immunoprecipitated SMC1, MBS3904078 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Goat Anti-SMC1 Polyclonal Antibody, Immunoprecipitation (IP) – Cat# MBS3904078

Western Blot (WB) of anti-SMC1 antibody

Detection of mouse SMC1 by western blot. Samples: Whole cell lysate (15 and 50 ug) from NIH 3T3 cells. Antibodies: Affinity purified goat anti-SMC1 antibody MBS3904078 (lot MBS3904078-1) used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Goat Anti-SMC1 Polyclonal Antibody, Western Blot (WB) – Cat# MBS3904078

Western Blot (WB) of anti-SMC1 antibody

Detection of human SMC1 by western blot. Samples: Whole cell lysate from HEK293T (15 and 50 ug), HeLa (H; 50 ug), and Jurkat (J; 50 ug) cells. Antibodies: Affinity purified goat anti-SMC1 antibody MBS3904078 (lot MBS3904078-1) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Goat Anti-SMC1 Polyclonal Antibody, Western Blot (WB) – Cat# MBS3904078

Other Notes

Small volumes of anti-SMC1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for SMC1 purchase

MBS3904078 is an antibody targeting SMC1. Also known as Goat anti-SMC1 Antibody, Affinity Purified. Reactive in Human, Mouse. Host: Goat.

Additional Details

NCBI/Uniprot data below describe general gene information for SMC1. It may not necessarily be applicable to this product.

NCBI GI #
30581135
NCBI GeneID
8243
NCBI Accession #
NP_006297.2
UniProt Primary Accession #
Q14683
UniProt Secondary Accession #
O14995; Q16351; Q2M228
UniProt Related Accession #
Q14683
GenBank Nucleotide #
NM_006306.2
NCBI Official Full Name
structural maintenance of chromosomes protein 1A
NCBI Official Synonym Full Names
structural maintenance of chromosomes 1A
NCBI Official Symbol
SMC1A
NCBI Official Synonym Symbols
SMC1; SMCB; CDLS2; SB1.8; SMC1L1; DXS423E; SMC1alpha
UniProt Protein Name
Structural maintenance of chromosomes protein 1A
UniProt Synonym Protein Names
Sb1.8
Protein Family
Structural maintenance of chromosomes protein
UniProt Gene Name
SMC1A
UniProt Synonym Gene Names
DXS423E; KIAA0178; SB1.8; SMC1; SMC1L1; SMC protein 1A; SMC-1-alpha; SMC-1A
UniProt Entry Name
SMC1A_HUMAN
NCBI Protein Information
structural maintenance of chromosomes protein 1A; SMC-1A; SMC-1-alpha; SMC protein 1A; segregation of mitotic chromosomes 1; SMC1 structural maintenance of chromosomes 1-like 1; SMC1 (structural maintenance of chromosomes 1, yeast)-like 1

NCBI Summary for SMC1

Proper cohesion of sister chromatids is a prerequisite for the correct segregation of chromosomes during cell division. The cohesin multiprotein complex is required for sister chromatid cohesion. This complex is composed partly of two structural maintenance of chromosomes (SMC) proteins, SMC3 and either SMC1L2 or the protein encoded by this gene. Most of the cohesin complexes dissociate from the chromosomes before mitosis, although those complexes at the kinetochore remain. Therefore, the encoded protein is thought to be an important part of functional kinetochores. In addition, this protein interacts with BRCA1 and is phosphorylated by ATM, indicating a potential role for this protein in DNA repair. This gene, which belongs to the SMC gene family, is located in an area of the X-chromosome that escapes X inactivation. [provided by RefSeq, Jul 2008]
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UniProt Comments for SMC1

Smc1: a component of the cohesin multiprotein complex that is required for sister chromatid cohesion. Most of the cohesin complexes dissociate from the chromosomes before mitosis, although those complexes at the kinetochore remain. Thought to be an important part of functional kinetochores. Interacts with BRCA1 and is phosphorylated by ATM, indicating a potential role for this protein in DNA repair.

Protein type: RNA splicing; DNA replication

Chromosomal Location of Human Ortholog: Xp11.22-p11.21

Cellular Component: kinetochore; nucleoplasm; cohesin core heterodimer; condensed nuclear chromosome; cytoplasm; nucleolus; chromosome; nucleus; cytosol; chromosome, pericentric region

Molecular Function: protein binding; protein heterodimerization activity; microtubule motor activity; chromatin binding; ATP binding

Biological Process: meiosis; mitotic sister chromatid segregation; mitotic cell cycle checkpoint; RNA splicing; stem cell maintenance; mitotic sister chromatid cohesion; DNA damage response, signal transduction; sister chromatid cohesion; negative regulation of DNA endoreduplication; DNA repair; nuclear mRNA splicing, via spliceosome; response to radiation; mitotic spindle organization and biogenesis; cell division; gene expression; mitotic cell cycle

Disease: Cornelia De Lange Syndrome 2
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Research Articles on SMC1

1. c-MYC down-regulation caused by cohesin mutations in SMC1A and SMC3 genes may be an early/primary event in the pathogenesis of Cornelia de Lange syndrome.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-SMC1 antibody

 Reference Product  PubMed Publications
 SMC3 antibody  >28 publications with SMC1 and SMC3
 RAD21 antibody  >9 publications with SMC1 and RAD21
 ATM antibody  >1 publications with SMC1 and ATM

Pathways associated with anti-SMC1 antibody

 Products by Pathway  Pathway Diagram
 Cell Cycle Pathway antibodies  Cell Cycle Pathway Diagram
 Cell Cycle, Mitotic Pathway antibodies  Cell Cycle, Mitotic Pathway Diagram
 Cell Cycle Pathway antibodies  Cell Cycle Pathway Diagram
 Cell Cycle Pathway antibodies  Cell Cycle Pathway Diagram
 Cell Cycle Pathway antibodies  Cell Cycle Pathway Diagram
 Chromosome Maintenance Pathway antibodies  Chromosome Maintenance Pathway Diagram
 Cohesin Loading Onto Chromatin Pathway antibodies  Cohesin Loading Onto Chromatin Pathway Diagram
 DNA Damage Response Pathway antibodies  DNA Damage Response Pathway Diagram
 Establishment Of Sister Chromatid Cohesion Pathway antibodies  Establishment Of Sister Chromatid Cohesion Pathway Diagram
 Gene Expression Pathway antibodies  Gene Expression Pathway Diagram

Diseases associated with anti-SMC1 antibody

 Disease Name  Pubmed Publications
 CdLS, X-Linked Antibodies  >37 publications with SMC1 and CdLS, X-Linked
 Neoplasms Antibodies  >5 publications with SMC1 and Neoplasms
 Heart Diseases Antibodies  >4 publications with SMC1 and Heart Diseases
 Death Antibodies  >4 publications with SMC1 and Death
 Cardiomyopathies Antibodies  >1 publications with SMC1 and Cardiomyopathies
 Precancerous Conditions Antibodies  >1 publications with SMC1 and Precancerous Conditions
 Immune System Diseases Antibodies  >1 publications with SMC1 and Immune System Diseases
 Hypertrophy Antibodies  >1 publications with SMC1 and Hypertrophy

Organs/Tissues associated with anti-SMC1 antibody

 Organ/Tissue Name  Pubmed Publications
 Bone Antibodies  >4 publications with SMC1 and Bone
 Heart Antibodies  >4 publications with SMC1 and Heart
 Blood Antibodies  >3 publications with SMC1 and Blood
 Muscle Antibodies  >2 publications with SMC1 and Muscle
 Mouth Antibodies  >2 publications with SMC1 and Mouth
 Lung Antibodies  >2 publications with SMC1 and Lung
 Bone Marrow Antibodies  >2 publications with SMC1 and Bone Marrow
 Eye Antibodies  >2 publications with SMC1 and Eye
 Esophagus Antibodies  >1 publications with SMC1 and Esophagus
 Embryonic Tissue Antibodies  >1 publications with SMC1 and Embryonic Tissue
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