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CARF Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS3904106

CARF (collaborator of ARF) is also known as CDKN2A interacting protein. CARF plays a role in regulating the p53 pathway as well as the senescence pathway. More details

$250 / 0.01 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
CARF, Polyclonal Antibody
Full Product Name
Rabbit anti-CARF Antibody, Affinity Purified
Product Gene Name
Product Synonym Gene Name
CDKN2AIP
Product Synonym Names
collaborator of ARF; collaborates/cooperates with ARF (alternate reading frame) protein; CARF; CDKN2A-interacting protein; CDKN2A-interacting protein; CDKN2A interacting protein
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Purity/Purification
Antigen Affinity Purified
Concentration
1000ug/ml (lot specific)
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Molecular Weight
61,125 Da
Chromosome Location
Chromosome: 4; NC_000004.11 (184365788..184369348). Location: 4q35.1
3D Structure
ModBase 3D Structure for Q9NXV6
Immunogen
Between 225 and 275
Conjugation
Unconjugated

Product Categories/Family for anti-CARF antibody

Applications Tested/Suitable for anti-CARF antibody
Western Blot (WB), Immunoprecipitation (IP)
Application Notes for anti-CARF antibody
WB: 1:1000-1:5000
IP: 6ug/mg lysate

Immunoprecipitation (IP) of anti-CARF antibody

Detection of human CARF by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HEK293T cells. Antibodies: Affinity purified rabbit anti-CARF antibody MBS3904106 (lot MBS3904106-1) used for IP at 6 ug/mg lysate. CARF was also immunoprecipitated by rabbit anti-CARF antibody MBS3904105. For blotting immunoprecipitated CARF, MBS3904106 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
Rabbit Anti-CARF Polyclonal Antibody – Cat# MBS3904106

Western Blot (WB) of anti-CARF antibody

Detection of human CARF by western blot. Samples: Whole cell lysate from HEK293T (15 and 50 ug), HeLa (H; 50 ug), and Jurkat (J; 50 ug) cells. Antibodies: Affinity purified rabbit anti-CARF antibody MBS3904106 (lot MBS3904106-1) used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Rabbit Anti-CARF Polyclonal Antibody, Western Blot (WB) – Cat# MBS3904106

Other Notes

Small volumes of anti-CARF antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CARF purchase

MBS3904106 is an antibody targeting CARF. Also known as Rabbit anti-CARF Antibody, Affinity Purified. Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for CARF. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
CDKN2A-interacting protein
NCBI Official Synonym Full Names
CDKN2A interacting protein
NCBI Official Symbol
CDKN2AIP
NCBI Official Synonym Symbols
CARF
UniProt Protein Name
CDKN2A-interacting protein
UniProt Synonym Protein Names
Collaborator of ARF
UniProt Gene Name
CDKN2AIP
UniProt Synonym Gene Names
CARF
UniProt Entry Name
CARF_HUMAN
NCBI Protein Information
CDKN2A-interacting protein; collaborator of ARF; collaborates/cooperates with ARF (alternate reading frame) protein

UniProt Comments for CARF

CDKN2AIP: co-localizes and interacts with p14ARF in the nucleolus. Co-regulated with ARF and cooperates with it in activating p53.

Protein type: Unknown function

Chromosomal Location of Human Ortholog: 4q35.1

Cellular Component: nucleoplasm; granular component; cytoplasm; nucleolus; nucleus

Molecular Function: protein binding; RNA binding; p53 binding

Biological Process: positive regulation of signal transduction; regulation of protein stability; negative regulation of cell growth; response to DNA damage stimulus; positive regulation of cell growth
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-CARF antibody

Organs/Tissues associated with anti-CARF antibody

 Organ/Tissue Name  Pubmed Publications
 Nerve Antibodies  >1 publications with CARF and Nerve
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