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BRG1/SMARCA4 Antibody – Goat Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS3904120

The SWI/SNF-related, matrix-associated, actin-dependent regulators of chromatin (SMARC), also called BRG1-associated factors (BAFs), have been identified as components of the human SWI/SNF-like chromatin-remodeling protein complexes. BRG1/SMARCA4 is an ATPase… More details

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Product Overview

Online Datasheet in PDF
Product Name
BRG1/SMARCA4, Polyclonal Antibody
Full Product Name
Goat anti-BRG1/SMARCA4 Antibody, Affinity Purified
Product Synonym Gene Name
SMARCA4
Product Synonym Names
SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 4; transcription activator BRG1; protein brahma homolog 1; protein BRG-1; RTPS2; SNF2; SNF2-beta; SNF2L4; SNF2LB; sucrose nonfermenting-like 4; mitotic growth and transcription activator; SWI2; SNF2-like 4; BRG1; MRD16; nuclear protein GRB1; BAF190; brahma protein-like 1; ATP-dependent helicase SMARCA4; BRG1-associated factor 190A; BRM/SWI2-related gene 1; CSS4; global transcription activator homologous sequence; homeotic gene regulator; hSNF2b; BAF190A; Transcription activator BRG1; SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4
Clonality
Polyclonal
Host
Goat
Species Reactivity
Human, Mouse
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Purity/Purification
Antigen Affinity Purified
Concentration
1000ug/ml (lot specific)
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Molecular Weight
181,261 Da
OMIM
603254
3D Structure
ModBase 3D Structure for P51532
Immunogen
Between 75 and 125
Conjugation
Unconjugated

Product Categories/Family for anti-BRG1/SMARCA4 antibody

Applications Tested/Suitable for anti-BRG1/SMARCA4 antibody
Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC)
Application Notes for anti-BRG1/SMARCA4 antibody
WB: 1:1000-1:5000
IP: 6ug/mg lysate
IHC: 1:500-1:2000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

Immunohistochemistry (IHC) of anti-BRG1/SMARCA4 antibody

Detection of mouse BRG1 by immunohistochemistry. Sample: FFPE section of mouse renal cell carcinoma. Antibody: Affinity purified goat anti-BRG1 (Cat. No. MBS3904120 lot 1) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB
Goat Anti-BRG1/SMARCA4 Polyclonal Antibody – Cat# MBS3904120

Immunohistochemistry (IHC) of anti-BRG1/SMARCA4 antibody

Detection of human BRG1 by immunohistochemistry. Sample: FFPE section of human colon carcinoma. Antibody: Affinity purified goat anti-BRG1 (Cat. No. MBS3904120 lot 1) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB
Goat Anti-BRG1/SMARCA4 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS3904120

Immunoprecipitation (IP) of anti-BRG1/SMARCA4 antibody

Detection of human BRG1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HEK293T cells. Antibodies: Affinity purified goat anti-BRG1 antibody MBS3904120 (lot MBS3904120-1) used for IP at 6 ug/mg lysate. BRG1 was also immunoprecipitated by rabbit anti-BRG1 antibody MBS3901327. For blotting immunoprecipitated BRG1, MBS3904120 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Goat Anti-BRG1/SMARCA4 Polyclonal Antibody, Immunoprecipitation (IP) – Cat# MBS3904120

Western Blot (WB) of anti-BRG1/SMARCA4 antibody

Detection of human and mouse BRG1 by western blot. Samples: Whole cell lysate from HEK293T (15 and 50 ug), HeLa (50ug), Jurkat (50ug), and mouse NIH 3T3 (50ug) cells. Antibodies: Affinity purified goat anti-BRG1 antibody MBS3904120 (lot MBS3904120-1) used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
Goat Anti-BRG1/SMARCA4 Polyclonal Antibody, Western Blot (WB) – Cat# MBS3904120

Other Notes

Small volumes of anti-BRG1/SMARCA4 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for BRG1/SMARCA4 purchase

MBS3904120 is an antibody targeting BRG1/SMARCA4. Also known as Goat anti-BRG1/SMARCA4 Antibody, Affinity Purified. Reactive in Human, Mouse. Host: Goat.

Additional Details

NCBI/Uniprot data below describe general gene information for BRG1/SMARCA4. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
Homo sapiens SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4 (SMARCA4), transcript variant 3, mRNA
NCBI Official Synonym Full Names
SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4
NCBI Official Symbol
SMARCA4
NCBI Official Synonym Symbols
BRG1; SNF2; SWI2; MRD16; RTPS2; BAF190; SNF2L4; SNF2LB; hSNF2b; BAF190A
UniProt Protein Name
Transcription activator BRG1
UniProt Synonym Protein Names
ATP-dependent helicase SMARCA4; BRG1-associated factor 190A; BAF190A; Mitotic growth and transcription activator; Protein BRG-1; Protein brahma homolog 1; SNF2-beta; SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 4
UniProt Gene Name
SMARCA4
UniProt Synonym Gene Names
BAF190A; BRG1; SNF2B; SNF2L4; BAF190A
UniProt Entry Name
SMCA4_HUMAN
NCBI Protein Information
transcription activator BRG1; ATP-dependent helicase SMARCA4; BRG1-associated factor 190A; BRM/SWI2-related gene 1; SNF2-beta; SNF2-like 4; brahma protein-like 1; global transcription activator homologous sequence; homeotic gene regulator; mitotic growth

NCBI Summary for BRG1/SMARCA4

The protein encoded by this gene is a member of the SWI/SNF family of proteins and is similar to the brahma protein of Drosophila. Members of this family have helicase and ATPase activities and are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The encoded protein is part of the large ATP-dependent chromatin remodeling complex SNF/SWI, which is required for transcriptional activation of genes normally repressed by chromatin. In addition, this protein can bind BRCA1, as well as regulate the expression of the tumorigenic protein CD44. Mutations in this gene cause rhabdoid tumor predisposition syndrome type 2. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2012]
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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