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PSME3 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS3904122

The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2… More details

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Product Overview

Online Datasheet in PDF
Product Name
PSME3, Polyclonal Antibody
Full Product Name
Rabbit anti-PSME3 Antibody, Affinity Purified
Product Gene Name
anti-PSME3 antibody
Product Synonym Names
PA28gamma; REG-GAMMA; REG gamma-3; 11S regulator complex gamma subunit; proteasome activator complex subunit 3; proteasome activator 28-gamma; proteasome activator 28 subunit gamma; proteasome (prosome, macropain) activator subunit 3 (PA28 gamma); PA28-gamma; PA28 gamma variant 5; Ki nuclear autoantigen; Ki antigen; Ki; HEL-S-283; epididymis secretory protein Li 283; 11S regulator complex subunit gamma; activator of multicatalytic protease subunit 3; PA28G; Proteasome activator complex subunit 3; proteasome activator subunit 3
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human, Mouse
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Purity/Purification
Antigen Affinity Purified
Concentration
1000ug/ml (lot specific)
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Molecular Weight
30,890 Da
OMIM
605129
3D Structure
ModBase 3D Structure for P61289
Predicted Reactivity
Chicken, Pig, Orangutan
Immunogen
Between 204 and C-terminus
Conjugation
Unconjugated

Product Categories/Family for anti-PSME3 antibody

Primary Antibodies
Applications Tested/Suitable for anti-PSME3 antibody
Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC)
Application Notes for anti-PSME3 antibody
WB: 1:500-1:2500
IP: 6ug/mg lysate
IHC: 1:500-1:2000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

Immunohistochemistry (IHC) of anti-PSME3 antibody

Detection of human PSME3 by immunohistochemistry. Sample: FFPE section of human non-small cell lung cancer. Antibody: Affinity purified rabbit anti-PSME3 (Cat. No. MBS3904122 Lot1) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB
Rabbit Anti-PSME3 Polyclonal Antibody – Cat# MBS3904122

Immunohistochemistry (IHC) of anti-PSME3 antibody

Detection of mouse PSME3 by immunohistochemistry. Sample: FFPE section of mouse renal cell carcinoma. Antibody: Affinity purified rabbit anti-PSME3 (Cat. No. MBS3904122 Lot1) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB
Rabbit Anti-PSME3 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS3904122

Immunoprecipitation (IP) of anti-PSME3 antibody

Detection of human PSME3 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HEK293T cells. Antibodies: Affinity purified rabbit anti-PSME3 antibody MBS3904122 (lot MBS3904122-1) used for IP at 6 ug/mg lysate. For blotting immunoprecipitated, MBS3904122 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Rabbit Anti-PSME3 Polyclonal Antibody, Immunoprecipitation (IP) – Cat# MBS3904122

Western Blot (WB) of anti-PSME3 antibody

Detection of human and mouse PSME3 by western blot. Samples: Whole cell lysate from HEK293T (15 and 50 ug), HeLa (50ug), Jurkat (50ug), and mouse NIH 3T3 (50ug) cells. Antibodies: Affinity purified rabbit anti-PSME3 antibody MBS3904122 (lot MBS3904122-1) used for WB at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Rabbit Anti-PSME3 Polyclonal Antibody, Western Blot (WB) – Cat# MBS3904122

Other Notes

Small volumes of anti-PSME3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for PSME3 purchase

MBS3904122 is an antibody targeting PSME3. Also known as Rabbit anti-PSME3 Antibody, Affinity Purified. Reactive in Human, Mouse. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for PSME3. It may not necessarily be applicable to this product.

NCBI GI #
30410794
NCBI GeneID
10197
NCBI Accession #
NP_005780.2
UniProt Primary Accession #
P61289
UniProt Secondary Accession #
O35563; P97373; Q12920; Q13172; Q9BQD9; A8K9A3
UniProt Related Accession #
P61289
GenBank Nucleotide #
NM_005789.3
NCBI Official Full Name
proteasome activator complex subunit 3 isoform 1
NCBI Official Synonym Full Names
proteasome activator subunit 3
NCBI Official Symbol
PSME3
NCBI Official Synonym Symbols
Ki; PA28G; HEL-S-283; PA28gamma; REG-GAMMA; PA28-gamma
UniProt Protein Name
Proteasome activator complex subunit 3
UniProt Synonym Protein Names
11S regulator complex subunit gamma; REG-gamma; Activator of multicatalytic protease subunit 3; Ki nuclear autoantigen; Proteasome activator 28 subunit gamma
Protein Family
Proteasome activator
UniProt Gene Name
PSME3
UniProt Synonym Gene Names
REG-gamma; PA28g; PA28gamma
UniProt Entry Name
PSME3_HUMAN
NCBI Protein Information
proteasome activator complex subunit 3

UniProt Comments for PSME3

PSME3: a protein implicated in immunoproteasome assembly and required for efficient antigen processing. The PA28 activator complex enhances the generation of class I binding peptides by altering the cleavage pattern of the proteasome. Homohexamer. Significantly elevated in colorectal cancer patients compared with healthy donors and patients with benign bowel disease. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Nuclear receptor co-regulator; Proteasome complex; Protease

Chromosomal Location of Human Ortholog: 17q21

Cellular Component: nucleoplasm; proteasome complex; proteasome activator complex; membrane; cytoplasm; nucleus; cytosol

Molecular Function: protein binding; p53 binding

Biological Process: positive regulation of ubiquitin-protein ligase activity during mitotic cell cycle; negative regulation of ubiquitin-protein ligase activity during mitotic cell cycle; protein polyubiquitination; viral reproduction; apoptosis; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent; DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest; regulation of apoptosis; antigen processing and presentation of peptide antigen via MHC class I; regulation of ubiquitin-protein ligase activity during mitotic cell cycle; anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; antigen processing and presentation of exogenous peptide antigen via MHC class I; gene expression; mitotic cell cycle; regulation of amino acid metabolic process; G1/S transition of mitotic cell cycle; negative regulation of apoptosis
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-PSME3 antibody

 Products by Pathway  Pathway Diagram
 APC/C-mediated Degradation Of Cell Cycle Proteins Pathway antibodies  APC/C-mediated Degradation Of Cell Cycle Proteins Pathway Diagram
 APC/C:Cdc20 Mediated Degradation Of Securin Pathway antibodies  APC/C:Cdc20 Mediated Degradation Of Securin Pathway Diagram
 APC/C:Cdc20 Mediated Degradation Of Mitotic Proteins Pathway antibodies  APC/C:Cdc20 Mediated Degradation Of Mitotic Proteins Pathway Diagram
 APC/C:Cdh1 Mediated Degradation Of Cdc20 And Other APC/C:Cdh1 Targeted Proteins In Late Mitosis/early G1 Pathway antibodies  APC/C:Cdh1 Mediated Degradation Of Cdc20 And Other APC/C:Cdh1 Targeted Proteins In Late Mitosis/early G1 Pathway Diagram
 APC:Cdc20 Mediated Degradation Of Cell Cycle Proteins Prior To Satisfation Of The Cell Cycle Checkpoint Pathway antibodies  APC:Cdc20 Mediated Degradation Of Cell Cycle Proteins Prior To Satisfation Of The Cell Cycle Checkpoint Pathway Diagram
 ARMS-mediated Activation Pathway antibodies  ARMS-mediated Activation Pathway Diagram
 AUF1 (hnRNP D0) Binds And Destabilizes MRNA Pathway antibodies  AUF1 (hnRNP D0) Binds And Destabilizes MRNA Pathway Diagram
 Activation Of APC/C And APC/C:Cdc20 Mediated Degradation Of Mitotic Proteins Pathway antibodies  Activation Of APC/C And APC/C:Cdc20 Mediated Degradation Of Mitotic Proteins Pathway Diagram
 Activation Of NF-kappaB In B Cells Pathway antibodies  Activation Of NF-kappaB In B Cells Pathway Diagram
 Adaptive Immune System Pathway antibodies  Adaptive Immune System Pathway Diagram

Diseases associated with anti-PSME3 antibody

 Disease Name  Pubmed Publications
 Adenocarcinoma Antibodies  >1 publications with PSME3 and Adenocarcinoma
 Inflammation Antibodies  >1 publications with PSME3 and Inflammation

Organs/Tissues associated with anti-PSME3 antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >2 publications with PSME3 and Blood
 Muscle Antibodies  >2 publications with PSME3 and Muscle
 Kidney Antibodies  >2 publications with PSME3 and Kidney
 Bone Antibodies  >1 publications with PSME3 and Bone
 Bone Marrow Antibodies  >1 publications with PSME3 and Bone Marrow
 Spleen Antibodies  >1 publications with PSME3 and Spleen
 Vascular Antibodies  >1 publications with PSME3 and Vascular
 Eye Antibodies  >1 publications with PSME3 and Eye
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