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ADAR1 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS3904127

ADAR1 (adenosine deaminase, RNA specific) is an enzyme responsible for RNA editing by site-specific deamination of adenosines. This enzyme destabilizes double-stranded RNA through conversion of adenosine to inosine. Mutations in this gene have been associated… More details

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Product Overview

Online Datasheet in PDF
Product Name
ADAR1, Polyclonal Antibody
Full Product Name
Rabbit anti-ADAR1 Antibody, Affinity Purified
Product Gene Name
anti-ADAR1 antibody
Product Synonym Gene Name
ADAR
Product Synonym Names
dsRNA adeonosine deaminase; ADAR1; K88DSRBP; interferon-inducible protein 4; interferon-induced protein 4; IFI-4; IFI4; G1P1; P136; dsRNA adenosine deaminase; DSRAD; DSH; DRADA; double-stranded RNA-specific adenosine deaminase; adenosine deaminase acting on RNA 1-A; 136 kDa double-stranded RNA-binding protein; AGS6; Double-stranded RNA-specific adenosine deaminase; adenosine deaminase, RNA specific
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Purity/Purification
Antigen Affinity Purified
Concentration
1000ug/ml (lot specific)
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
OMIM
127400
3D Structure
ModBase 3D Structure for P55265
Predicted Reactivity
Mouse, Rat
Immunogen
Between 975 and 1025
Conjugation
Unconjugated

Product Categories/Family for anti-ADAR1 antibody

Primary Antibodies
Applications Tested/Suitable for anti-ADAR1 antibody
Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC)
Application Notes for anti-ADAR1 antibody
WB: 1:1000-1:5000
IP: 6ug/mg lysate
IHC: 1:500-1:2000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

Immunohistochemistry (IHC) of anti-ADAR1 antibody

Detection of human ADAR1 by immunohistochemistry. Samples: FFPE sections of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-ADAR1 Cat. No. MBS3904126 Lot1 used at a dilution of 1:1,000 (1ug/ml) (left) and Cat. No. MBS3904127 Lot1 used at a dilution of 1:1,000 (1ug/ml) (right). Detection: DAB
Rabbit Anti-ADAR1 Polyclonal Antibody – Cat# MBS3904127

Immunoprecipitation (IP) of anti-ADAR1 antibody

Detection of human ADAR1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HEK293T cells. Antibodies: Affinity purified rabbit anti-ADAR1 antibody MBS3904127 (lot MBS3904127-1) used for IP at 6 ug/mg lysate. ADAR1 was also immunoprecipitated by rabbit anti-ADAR1 antibody MBS3904126. For blotting immunoprecipitated ADAR1, MBS3901395 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Rabbit Anti-ADAR1 Polyclonal Antibody, Immunoprecipitation (IP) – Cat# MBS3904127

Western Blot (WB) of anti-ADAR1 antibody

Detection of human ADAR1 by western blot. Samples: Whole cell lysate from HEK293T (15 and 50 ug), HeLa (50 ug), and Jurkat (50ug) cells. Antibodies: Affinity purified rabbit anti-ADAR1 antibody MBS3904127 (lot MBS3904127-1) used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
Rabbit Anti-ADAR1 Polyclonal Antibody, Western Blot (WB) – Cat# MBS3904127

Other Notes

Small volumes of anti-ADAR1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for ADAR1 purchase

MBS3904127 is an antibody targeting ADAR1. Also known as Rabbit anti-ADAR1 Antibody, Affinity Purified. Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for ADAR1. It may not necessarily be applicable to this product.

NCBI GeneID
103
NCBI Accession #
NP_056656.3
UniProt Primary Accession #
P55265
UniProt Protein Name
Double-stranded RNA-specific adenosine deaminase
UniProt Synonym Protein Names
136 kDa double-stranded RNA-binding protein; p136; Interferon-inducible protein 4; IFI-4; K88DSRBP
UniProt Gene Name
ADAR
UniProt Synonym Gene Names
ADAR1; DSRAD; G1P1; IFI4; DRADA; p136; IFI-4
UniProt Entry Name
DSRAD_HUMAN

UniProt Comments for ADAR1

ADAR: Converts multiple adenosines to inosines and creates I/U mismatched base pairs in double-helical RNA substrates without apparent sequence specificity. Has been found to modify more frequently adenosines in AU-rich regions, probably due to the relative ease of melting A/U base pairs as compared to G/C pairs. Functions to modify viral RNA genomes and may be responsible for hypermutation of certain negative-stranded viruses. Edits the messenger RNAs for glutamate receptor (GLUR) subunits by site- selective adenosine deamination. Produces low-level editing at the GLUR-B Q/R site, but edits efficiently at the R/G site and HOTSPOT1. Binds to short interfering RNAs (siRNA) without editing them and suppresses siRNA-mediated RNA interference. Binds to ILF3/NF90 and up-regulates ILF3-mediated gene expression. Isoform 1 is induced by interferon alpha. Isoform 5 is constitutively expressed. Homodimer. Isoform 1 interacts with ILF2/NF45 and ILF3/NF90. 5 isoforms of the human protein are produced by alternative promoter.

Protein type: RNA-binding; RNA processing; Nucleolus; Hydrolase; EC 3.5.4.37

Chromosomal Location of Human Ortholog: 1q21.3

Cellular Component: nucleoplasm; membrane; cytoplasm; nucleolus; nucleus

Molecular Function: double-stranded RNA adenosine deaminase activity; protein binding; DNA binding; metal ion binding

Biological Process: positive regulation of viral genome replication; base conversion or substitution editing; in utero embryonic development; response to virus; cytokine and chemokine mediated signaling pathway; miRNA-mediated gene silencing, miRNA loading onto RISC; somatic diversification of immune receptors via somatic mutation; adenosine to inosine editing; osteoblast differentiation; negative regulation of viral genome replication; protein import into nucleus; pre-microRNA processing; mRNA modification; innate immune response; erythrocyte differentiation; gene expression; hemopoietic progenitor cell differentiation; protein export from nucleus; mRNA processing; defense response to virus; negative regulation of apoptosis

Disease: Dyschromatosis Symmetrica Hereditaria; Aicardi-goutieres Syndrome 6
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
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