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TIA-1 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS3904226

TIA-1 (T-cell-restricted intracellular antigen-1) is involved in alternative pre-RNA splicing and regulation of mRNA translation by binding to AU-rich elements (AREs) located in mRNA 3' untranslated regions (3' UTRs). TIA-1 possesses nucleolytic activity… More details

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Product Overview

Online Datasheet in PDF
Product Name
TIA-1, Polyclonal Antibody
Full Product Name
Rabbit anti-TIA-1 Antibody, Affinity Purified
Product Gene Name
Product Synonym Gene Name
TIA1
Product Synonym Names
nucleolysin TIA-1 isoform p40; WDM; TIA-1; T-cell-restricted intracellular antigen-1; RNA-binding protein TIA-1; p40-TIA-1; nucleolysin TIA-1; p40-TIA-1 (containing p15-TIA-1); Nucleolysin TIA-1 isoform p40; TIA1 cytotoxic granule associated RNA binding protein
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human, Mouse
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Purity/Purification
Antigen Affinity Purified
Concentration
1000ug/ml (lot specific)
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
OMIM
gene 604454
3D Structure
ModBase 3D Structure for P31483
Immunogen
Between 1 and 50
Conjugation
Unconjugated

Product Categories/Family for anti-TIA-1 antibody

Applications Tested/Suitable for anti-TIA-1 antibody
Western Blot (WB)
Application Notes for anti-TIA-1 antibody
WB: 1:2000-1:10,000

Western Blot (WB) of anti-TIA-1 antibody

Detection of human and mouse TIA-1 by western blot. Samples: Whole cell lysate from HeLa (15 ug), HEK293T (15 ug), Jurkat (15 ug), mouse TCMK-1 (50ug), and mouse NIH 3T3 (50ug) cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-TIA-1 antibody MBS3904226 (lot MBS3904226-2) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Rabbit Anti-TIA-1 Polyclonal Antibody – Cat# MBS3904226

Other Notes

Small volumes of anti-TIA-1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for TIA-1 purchase

MBS3904226 is an antibody targeting TIA-1. Also known as Rabbit anti-TIA-1 Antibody, Affinity Purified. Reactive in Human, Mouse. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for TIA-1. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
nucleolysin TIA-1 isoform p40 isoform 1
NCBI Official Synonym Full Names
TIA1 cytotoxic granule associated RNA binding protein
NCBI Official Symbol
TIA1
NCBI Official Synonym Symbols
WDM; TIA-1
UniProt Protein Name
Nucleolysin TIA-1 isoform p40
UniProt Synonym Protein Names
RNA-binding protein TIA-1; T-cell-restricted intracellular antigen-1; TIA-1; p40-TIA-1
Protein Family
UniProt Gene Name
TIA1
UniProt Synonym Gene Names
TIA-1
UniProt Entry Name
TIA1_HUMAN
NCBI Protein Information
nucleolysin TIA-1 isoform p40; nucleolysin TIA-1

NCBI Summary for TIA-1

The product encoded by this gene is a member of a RNA-binding protein family and possesses nucleolytic activity against cytotoxic lymphocyte (CTL) target cells. It has been suggested that this protein may be involved in the induction of apoptosis as it preferentially recognizes poly(A) homopolymers and induces DNA fragmentation in CTL targets. The major granule-associated species is a 15-kDa protein that is thought to be derived from the carboxyl terminus of the 40-kDa product by proteolytic processing. Alternative splicing resulting in different isoforms has been found for this gene. [provided by RefSeq, May 2017]
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UniProt Comments for TIA-1

TIA1: Involved in alternative pre-RNA splicing and regulation of mRNA translation by binding to AU-rich elements (AREs) located in mRNA 3' untranslated regions (3' UTRs). Possesses nucleolytic activity against cytotoxic lymphocyte target cells. May be involved in apoptosis. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Translation; RNA-binding

Chromosomal Location of Human Ortholog: 2p13

Cellular Component: nucleoplasm; stress granule; cytoplasm

Molecular Function: protein binding; nucleotide binding; AU-rich element binding; poly(A) binding

Biological Process: regulation of nuclear mRNA splicing, via spliceosome; apoptosis; negative regulation of translation; negative regulation of cytokine biosynthetic process

Disease: Welander Distal Myopathy
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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