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NRF Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS3904251

NFkappaB repressing factor (NRF) is a transcriptional repressor that interacts with specific negative regulatory elements to mediate transcriptional repression of certain nuclear factor kappa B responsive genes. NRF localizes predominantly to the nucleolus… More details

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Sample Manual Insert View PDF
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Testing Data View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
NRF, Polyclonal Antibody
Full Product Name
Rabbit anti-NRF Antibody, Affinity Purified
Product Gene Name
Product Synonym Gene Name
NKRF
Product Synonym Names
ITBA4; NF-kappa-B-repressing factor; NFkB-repressing factor; NRF; Protein ITBA4; transcription factor NRF; NF-kappa-B-repressing factor; NFKB repressing factor
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Purity/Purification
Antigen Affinity Purified
Concentration
1000ug/ml (lot specific)
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Molecular Weight
77,673 Da
Chromosome Location
Chromosome: X; NC_000023.10 (118722300..118739846, complement). Location: Xq24
OMIM
300440
3D Structure
ModBase 3D Structure for O15226
Immunogen
Between 50 and 100
Conjugation
Unconjugated

Product Categories/Family for anti-NRF antibody

Applications Tested/Suitable for anti-NRF antibody
Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC)
Application Notes for anti-NRF antibody
WB: 1:2000-1:10,000
IP: 6ug/mg lysate
IHC: 1:500-1:2000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

Immunohistochemistry (IHC) of anti-NRF antibody

Detection of human NRF by immunohistochemistry. Sample: FFPE section of human non-small cell lung cancer. Antibody: Affinity purified rabbit anti-NRF (Cat. No. MBS3904251 Lot1) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB
Rabbit Anti-NRF Polyclonal Antibody – Cat# MBS3904251

Immunoprecipitation (IP) of anti-NRF antibody

Detection of human NRF by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-NRF antibody MBS3904251 (lot MBS3904251-1) used for IP at 6 ug/mg lysate. NRF was also immunoprecipitated by rabbit anti-NRF antibody BL14509. For blotting immunoprecipitated NRF, MBS3904251 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
Rabbit Anti-NRF Polyclonal Antibody, Immunoprecipitation (IP) – Cat# MBS3904251

Western Blot (WB) of anti-NRF antibody

Detection of human NRF by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, and Jurkat cells. Antibodies: Affinity purified rabbit anti-NRF antibody MBS3904251 (lot MBS3904251-1) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Rabbit Anti-NRF Polyclonal Antibody, Western Blot (WB) – Cat# MBS3904251

Other Notes

Small volumes of anti-NRF antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for NRF purchase

MBS3904251 is an antibody targeting NRF. Also known as Rabbit anti-NRF Antibody, Affinity Purified. Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for NRF. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
NF-kappa-B-repressing factor isoform 2
NCBI Official Synonym Full Names
NFKB repressing factor
NCBI Official Symbol
NKRF
NCBI Official Synonym Symbols
NRF; ITBA4
UniProt Protein Name
NF-kappa-B-repressing factor
UniProt Synonym Protein Names
Protein ITBA4; Transcription factor NRF
UniProt Gene Name
NKRF
UniProt Synonym Gene Names
ITBA4; NRF; NFkB-repressing factor
UniProt Entry Name
NKRF_HUMAN
NCBI Protein Information
NF-kappa-B-repressing factor; OTTHUMP00000023906; OTTHUMP00000023907; transcription factor NRF

NCBI Summary for NRF

This gene encodes a transcriptional repressor that interacts with specific negative regulatory elements to mediate transcriptional repression of certain nuclear factor kappa B responsive genes. The protein localizes predominantly to the nucleolus with a small fraction found in the nucleoplasm and cytoplasm. Alternate splicing results in multiple transcript variants. [provided by RefSeq]
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UniProt Comments for NRF

NKRF: Interacts with a specific negative regulatory element (NRE) 5'-AATTCCTCTGA-3' to mediate transcriptional repression of certain NK-kappa-B responsive genes. Involved in the constitutive silencing of the interferon beta promoter, independently of the virus-induced signals, and in the inhibition of the basal and cytokine-induced iNOS promoter activity. Also involved in the regulation of IL-8 transcription.

Protein type: RNA-binding; Transcription, coactivator/corepressor; Nucleolus

Chromosomal Location of Human Ortholog: Xq24

Cellular Component: endoplasmic reticulum; nucleolus; nucleus

Molecular Function: protein binding; DNA binding

Biological Process: transcription, DNA-dependent; negative regulation of transcription, DNA-dependent
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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