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NHP2L1 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS3904258

NHP2 non-histone chromosome protein 2-like 1 (NHP2L1) was originally named because of its sequence similarity to the Saccharomyces cerevisiae NHP2 (non-histone protein 2), this protein appears to be a highly conserved nuclear protein that is a component of… More details

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Product Overview

Online Datasheet in PDF
Product Name
NHP2L1, Polyclonal Antibody
Full Product Name
Rabbit anti-NHP2L1 Antibody, Affinity Purified
Product Gene Name
Product Synonym Gene Name
SNU13
Product Synonym Names
FA1; U4/U6.U5 tri-snRNP 15.5 kDa protein; U4/U6.U5 small nuclear ribonucleoprotein SNU13; SSFA1; sperm specific antigen 1; OTK27; SPAG12; SNU13 homolog, small nuclear ribonucleoprotein (U4/U6.U5); SNU13 homolog; SNRNP15-5; NHPX; NHP2-like protein 1; NHP2L1; NHP2 non-histone chromosome protein 2-like 1; hSNU13; FA-1; high mobility group-like nuclear protein 2 homolog 1; 15.5K; [U4/U6.U5] tri-snRNP 15.5 kD RNA binding protein; non-histone chromosome protein 2-like 1; NHP2-like protein 1; small nuclear ribonucleoprotein 13
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human, Mouse
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Purity/Purification
Antigen Affinity Purified
Concentration
1000ug/ml (lot specific)
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
OMIM
601304
3D Structure
ModBase 3D Structure for P55769
Predicted Reactivity
Rat, X. tropicalis, Bovine
Immunogen
Between 78 and C-terminus
Conjugation
Unconjugated

Product Categories/Family for anti-NHP2L1 antibody

Applications Tested/Suitable for anti-NHP2L1 antibody
Immunoprecipitation (IP), Immunohistochemistry (IHC)
Application Notes for anti-NHP2L1 antibody
IP: 6ug/mg lysate
IHC: 1:1000-1:5000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

Immunohistochemistry (IHC) of anti-NHP2L1 antibody

Detection of mouse NHP2L1 by immunohistochemistry. Sample: FFPE section of mouse renal cell carcinoma. Antibody: Affinity purified rabbit anti-NHP2L1 (Cat. No. MBS3904258 Lot1) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB
Rabbit Anti-NHP2L1 Polyclonal Antibody – Cat# MBS3904258

Immunohistochemistry (IHC) of anti-NHP2L1 antibody

Detection of human NHP2L1 by immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-NHP2L1 (Cat. No. MBS3904258 Lot1) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB. Counterstain: IHC Hematoxylin (blue).
Rabbit Anti-NHP2L1 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS3904258

Immunoprecipitation (IP) of anti-NHP2L1 antibody

Detection of human NHP2L1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-NHP2L1 antibody MBS3904258 (lot MBS3904258-1) used for IP at 6 ug/mg lysate. NHP2L1 was also immunoprecipitated by rabbit anti-NHP2L1 antibody BL14339. For blotting immunoprecipitated NHP2L1, MBS3904258 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Rabbit Anti-NHP2L1 Polyclonal Antibody, Immunoprecipitation (IP) – Cat# MBS3904258

Other Notes

Small volumes of anti-NHP2L1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for NHP2L1 purchase

MBS3904258 is an antibody targeting NHP2L1. Also known as Rabbit anti-NHP2L1 Antibody, Affinity Purified. Reactive in Human, Mouse. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for NHP2L1. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
NHP2-like protein 1
NCBI Official Synonym Full Names
small nuclear ribonucleoprotein 13
NCBI Official Symbol
SNU13
NCBI Official Synonym Symbols
FA1; FA-1; NHPX; 15.5K; OTK27; SSFA1; NHP2L1; SPAG12; SNRNP15-5
UniProt Protein Name
NHP2-like protein 1
UniProt Synonym Protein Names
High mobility group-like nuclear protein 2 homolog 1; OTK27; SNU13 homolog; hSNU13; U4/U6.U5 tri-snRNP 15.5 kDa proteinNHP2-like protein 1, N-terminally processed
UniProt Gene Name
NHP2L1
UniProt Synonym Gene Names
hSNU13
UniProt Entry Name
NH2L1_HUMAN
NCBI Protein Information
NHP2-like protein 1

NCBI Summary for NHP2L1

Originally named because of its sequence similarity to the Saccharomyces cerevisiae NHP2 (non-histone protein 2), this protein appears to be a highly conserved nuclear protein that is a component of the [U4/U6.U5] tri-snRNP. It binds to the 5' stem-loop of U4 snRNA. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]
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UniProt Comments for NHP2L1

NHP2L1: Binds to the 5'-stem-loop of U4 snRNA and may play a role in the late stage of spliceosome assembly. The protein undergoes a conformational change upon RNA-binding. Belongs to the ribosomal protein L7Ae family.

Protein type: RNA splicing; Nucleolus; Spliceosome; RNA-binding

Chromosomal Location of Human Ortholog: 22q13

Cellular Component: nucleoplasm; spliceosome; protein complex; nucleolus; nucleus

Molecular Function: snoRNA binding; protein binding; RNA binding

Biological Process: nuclear mRNA splicing, via spliceosome; RNA splicing; ribosome biogenesis and assembly; gene expression
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-NHP2L1 antibody

Diseases associated with anti-NHP2L1 antibody

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