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GOLGB1 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS3904260

Golgin subfamily B member 1 (GOLGB1) is a single pass type I membrane protein located in the golgi apparatus membrane. GOLGB1 may participate in forming intercisternal cross-bridges of the Golgi complex [taken from the Universal Protein Resource (UniProt)… More details

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Product Overview

Online Datasheet in PDF
Product Name
GOLGB1, Polyclonal Antibody
Full Product Name
Rabbit anti-GOLGB1 Antibody, Affinity Purified
Product Gene Name
Product Synonym Names
golgi autoantigen, golgin subfamily b, macrogolgin (with transmembrane signal), 1; Macrogolgin; GOLIM1; golgin subfamily B member 1; golgi integral membrane protein 1; giantin; GCP372; GCP; 372 kDa Golgi complex-associated protein; golgin B1, golgi integral membrane protein; Golgin subfamily B member 1; golgin B1
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Purity/Purification
Antigen Affinity Purified
Concentration
1000ug/ml (lot specific)
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Molecular Weight
367,423 Da
3D Structure
ModBase 3D Structure for Q14789
Immunogen
Between 425 and 475
Conjugation
Unconjugated

Product Categories/Family for anti-GOLGB1 antibody

Applications Tested/Suitable for anti-GOLGB1 antibody
Western Blot (WB), Immunoprecipitation (IP)
Application Notes for anti-GOLGB1 antibody
WB: 1:2000-1:10,000 (3-8% Tris-acetate gel recommended)
IP: 6ug/mg lysate

Immunoprecipitation (IP) of anti-GOLGB1 antibody

Detection of human GOLGB1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HEK293T cells. Antibodies: Affinity purified rabbit anti-GOLGB1 antibody MBS3904260 (lot MBS3904260-1) used for IP at 6 ug/mg lysate. GOLGB1 was also immunoprecipitated by rabbit anti-GOLGB1 antibodies MBS3904261 and MBS3904262. For blotting immunoprecipitated GOLGB1, MBS3904260 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
Rabbit Anti-GOLGB1 Polyclonal Antibody – Cat# MBS3904260

Western Blot (WB) of anti-GOLGB1 antibody

Detection of human GOLGB1 by western blot. Samples: Whole cell lysate (50 ug) from HEK293T, HeLa, and Jurkat cells. Antibodies: Affinity purified rabbit anti-GOLGB1 antibody MBS3904260 (lot MBS3904260-1) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
Rabbit Anti-GOLGB1 Polyclonal Antibody, Western Blot (WB) – Cat# MBS3904260

Other Notes

Small volumes of anti-GOLGB1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for GOLGB1 purchase

MBS3904260 is an antibody targeting GOLGB1. Also known as Rabbit anti-GOLGB1 Antibody, Affinity Purified. Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for GOLGB1. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
Homo sapiens golgin B1 (GOLGB1), transcript variant 2, mRNA
NCBI Official Synonym Full Names
golgin B1
NCBI Official Symbol
GOLGB1
NCBI Official Synonym Symbols
GCP; GCP372; GOLIM1
UniProt Protein Name
Golgin subfamily B member 1
UniProt Synonym Protein Names
372 kDa Golgi complex-associated protein; GCP372; Giantin; Macrogolgin
Protein Family
UniProt Gene Name
GOLGB1
UniProt Synonym Gene Names
GCP372
NCBI Protein Information
Golgin subfamily B member 1; 372 kDa Golgi complex-associated protein; giantin; golgi autoantigen, golgin subfamily b, macrogolgin (with transmembrane signal), 1; golgi integral membrane protein 1; golgin B1, golgi integral membrane protein

UniProt Comments for GOLGB1

May participate in forming intercisternal cross-bridges of the Golgi complex.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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