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EDF-1 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS3904266

Endothelial differentiation-related factor 1 (EDF-1) is a protein that may regulate endothelial cell differentiation. It has been postulated that the protein functions as a bridging molecule that interconnects regulatory proteins and the basal transcriptional… More details

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Product Overview

Online Datasheet in PDF
Product Name
EDF-1, Polyclonal Antibody
Full Product Name
Rabbit anti-EDF-1 Antibody, Affinity Purified
Product Gene Name
anti-EDF-1 antibody
Product Synonym Gene Name
EDF1
Product Synonym Names
Multiprotein-bridging factor 1; MBF1; endothelial differentiation-related factor 1; EDF-1; CFAP280; multiprotein bridging factor 1; Endothelial differentiation-related factor 1; endothelial differentiation related factor 1
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Purity/Purification
Antigen Affinity Purified
Concentration
1000ug/ml (lot specific)
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Molecular Weight
17.4 kDa (156aa) confirmed by MALDI-TOF
OMIM
605107
3D Structure
ModBase 3D Structure for O60869
Predicted Reactivity
Bovine
Immunogen
Between 98 and C-terminus
Conjugation
Unconjugated

Product Categories/Family for anti-EDF-1 antibody

Primary Antibodies
Applications Tested/Suitable for anti-EDF-1 antibody
Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC)
Application Notes for anti-EDF-1 antibody
WB: 1:2000-1:10,000
IP: 6ug/mg lysate
IHC: 1:500-1:2000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

Immunohistochemistry (IHC) of anti-EDF-1 antibody

Detection of human EDF-1 by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-EDF-1 (Cat. No. MBS3904266 Lot1) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB
Rabbit Anti-EDF-1 Polyclonal Antibody – Cat# MBS3904266

Immunoprecipitation (IP) of anti-EDF-1 antibody

Detection of human EDF-1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HEK293T cells. Antibodies: Affinity purified rabbit anti-EDF-1 antibody MBS3904266 (lot MBS3904266-1) used for IP at 6 ug/mg lysate. EDF-1 was also immunoprecipitated by rabbit anti-EDF-1 antibody BL14527. For blotting immunoprecipitated EDF-1, MBS3904266 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.
Rabbit Anti-EDF-1 Polyclonal Antibody, Immunoprecipitation (IP) – Cat# MBS3904266

Western Blot (WB) of anti-EDF-1 antibody

Detection of human EDF-1 by western blot. Samples: Whole cell lysate (50 ug) from HEK293T, HeLa, and Jurkat cells. Antibodies: Affinity purified rabbit anti-EDF-1 antibody MBS3904266 (lot MBS3904266-1) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Rabbit Anti-EDF-1 Polyclonal Antibody, Western Blot (WB) – Cat# MBS3904266

Other Notes

Small volumes of anti-EDF-1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for EDF-1 purchase

MBS3904266 is an antibody targeting EDF-1. Also known as Rabbit anti-EDF-1 Antibody, Affinity Purified. Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for EDF-1. It may not necessarily be applicable to this product.

NCBI GI #
24497601
NCBI GeneID
8721
NCBI Accession #
NP_694880.1
UniProt Primary Accession #
O60869
UniProt Secondary Accession #
Q5T5T2; Q9UIM1
UniProt Related Accession #
O60869
GenBank Nucleotide #
NM_153200.1
NCBI Official Full Name
endothelial differentiation-related factor 1 isoform beta
NCBI Official Synonym Full Names
endothelial differentiation-related factor 1
NCBI Official Symbol
EDF1
NCBI Official Synonym Symbols
MBF1; EDF-1
UniProt Protein Name
Endothelial differentiation-related factor 1
UniProt Synonym Protein Names
Multiprotein-bridging factor 1; MBF1
Protein Family
Endothelial differentiation-related factor
UniProt Gene Name
EDF1
UniProt Synonym Gene Names
EDF-1; MBF1
UniProt Entry Name
EDF1_HUMAN
NCBI Protein Information
endothelial differentiation-related factor 1

UniProt Comments for EDF-1

EDF1: Transcriptional coactivator stimulating NR5A1 and ligand-dependent NR1H3/LXRA and PPARG transcriptional activities. Enhances the DNA-binding activity of ATF1, ATF2, CREB1 and NR5A1. Regulates nitric oxid synthase activity probably by sequestering calmodulin in the cytoplasm. May function in endothelial cells differentiation, hormone-induced cardiomyocytes hypertrophy and lipid metabolism. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Transcription, coactivator/corepressor; DNA-binding; Nuclear receptor co-regulator; RNA-binding

Chromosomal Location of Human Ortholog: 9q34.3

Cellular Component: transcription factor TFIID complex; cytoplasm; nucleolus; intracellular; nucleus

Molecular Function: calmodulin binding; methyltransferase activity; protein binding; histone acetyltransferase activity; sequence-specific DNA binding; transcription coactivator activity; transcription factor activity

Biological Process: endothelial cell differentiation; regulation of lipid metabolic process; transcription, DNA-dependent; regulation of transcription, DNA-dependent; positive regulation of transcription, DNA-dependent; multicellular organismal development; positive regulation of DNA binding
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-EDF-1 antibody

 Reference Product  PubMed Publications
 JUN antibody  >10 publications with EDF-1 and JUN
 TBP antibody  >6 publications with EDF-1 and TBP
 NR5A1 antibody  >1 publications with EDF-1 and NR5A1
 NR5A2 antibody  >1 publications with EDF-1 and NR5A2

Diseases associated with anti-EDF-1 antibody

 Disease Name  Pubmed Publications
 Breast Neoplasms Antibodies  >2 publications with EDF-1 and Breast Neoplasms
 Ovarian Diseases Antibodies  >1 publications with EDF-1 and Ovarian Diseases
 Atherosclerosis Antibodies  >1 publications with EDF-1 and Atherosclerosis
 Cardiovascular Diseases Antibodies  >1 publications with EDF-1 and Cardiovascular Diseases
 Heart Diseases Antibodies  >1 publications with EDF-1 and Heart Diseases
 Hypertrophy Antibodies  >1 publications with EDF-1 and Hypertrophy

Organs/Tissues associated with anti-EDF-1 antibody

 Organ/Tissue Name  Pubmed Publications
 Vascular Antibodies  >8 publications with EDF-1 and Vascular
 Embryonic Tissue Antibodies  >3 publications with EDF-1 and Embryonic Tissue
 Umbilical Cord Antibodies  >2 publications with EDF-1 and Umbilical Cord
 Brain Antibodies  >2 publications with EDF-1 and Brain
 Ear Antibodies  >1 publications with EDF-1 and Ear
 Ovary Antibodies  >1 publications with EDF-1 and Ovary
 Muscle Antibodies  >1 publications with EDF-1 and Muscle
 Heart Antibodies  >1 publications with EDF-1 and Heart
 Liver Antibodies  >1 publications with EDF-1 and Liver
 Skin Antibodies  >1 publications with EDF-1 and Skin
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