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KAP-1 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS3904356

KAP-1 (KRAB-Associated Protein 1) is a member of the tripartite motif RBCC/Trim domain family that contains a RING finger domain, B boxes, and an alpha helical coiled coil region. KAP-1 functions as a corepressor by interacting with the KRAB domain of KRAB… More details

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Sample Manual Insert View PDF
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Testing Data View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
KAP-1, Polyclonal Antibody
Full Product Name
Rabbit anti-Phospho KAP-1 (S824) Antibody, Affinity Purified
Product Gene Name
Product Synonym Gene Name
Trim28
Product Synonym Names
KAP-1, Phospho (S824); E3 SUMO-protein ligase TRIM28; Tif1beta; tripartite motif-containing protein 28; tripartite motif protein 28; transcriptional intermediary factor 1, beta; transcription intermediary factor 1-beta; TIF1-beta; RING-type E3 ubiquitin transferase TIF1-beta; MommeD9; KRIP-1; AA408787; KAP-1; Tif1b; KRAB-A-interacting protein; Transcription intermediary factor 1-beta; tripartite motif-containing 28
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Mouse
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Purity/Purification
Antigen Affinity Purified
Concentration
1000ug/ml (lot specific)
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Molecular Weight
88,847 Da
3D Structure
ModBase 3D Structure for Q62318
Predicted Reactivity
Rat
Immunogen
Surrounding serine 824
Conjugation
Unconjugated

Product Categories/Family for anti-KAP-1 antibody

Applications Tested/Suitable for anti-KAP-1 antibody
Western Blot (WB), Immunoprecipitation (IP)
Application Notes for anti-KAP-1 antibody
WB: 1:2000-1:10,000
IP: 6ug/mg lysate

Immunoprecipitation (IP) of anti-KAP-1 antibody

Detection of mouse Phospho KAP-1 (S824) by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from TCMK cells treated with etoposide (+) and/or Lambda PP (+) or mock treated (-). Antibodies: Affinity purified rabbit anti- KAP-1 antibody (MBS3904355) used for IP at 6 ug per reaction. For blotting immunoprecipitated Phospho KAP-1 (S824), MBS3904356 lot 3 was used at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 1 second. Lower Panel: Rabbit anti-KAP-1 antibody (MBS3904355).
Rabbit Anti-KAP-1 Polyclonal Antibody – Cat# MBS3904356

Western Blot (WB) of anti-KAP-1 antibody

Detection of mouse Phospho KAP-1 (S824) by western blot. Samples: Whole cell lysate (10 ug) from BeWo and AlphaTC1 Clone 9 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Phospho KAP-1 (S824) antibody MBS3904356 (lot MBS3904356-3) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 1 second.
Rabbit Anti-KAP-1 Polyclonal Antibody, Western Blot (WB) – Cat# MBS3904356

Other Notes

Small volumes of anti-KAP-1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for KAP-1 purchase

MBS3904356 is an antibody targeting KAP-1. Also known as Rabbit anti-Phospho KAP-1 (S824) Antibody, Affinity Purified. Reactive in Mouse. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for KAP-1. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
transcription intermediary factor 1-beta
NCBI Official Synonym Full Names
tripartite motif-containing 28
NCBI Official Symbol
Trim28
NCBI Official Synonym Symbols
KAP-1; Tif1b; KRIP-1; MommeD9; AA408787; Tif1beta
UniProt Protein Name
Transcription intermediary factor 1-beta
UniProt Synonym Protein Names
E3 SUMO-protein ligase TRIM28 (EC:6.3.2.-); KRAB-A-interacting protein; KRIP-1; Tripartite motif-containing protein 28
UniProt Gene Name
Trim28
UniProt Synonym Gene Names
Kap1; Krip1; Tif1b; TIF1-beta
UniProt Entry Name
TIF1B_MOUSE
NCBI Protein Information
transcription intermediary factor 1-beta; TIF1-beta; KRAB-A-interacting protein; tripartite motif protein 28; E3 SUMO-protein ligase TRIM28; tripartite motif-containing protein 28; transcriptional intermediary factor 1, beta

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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