Rabbit Anti-RRP8 Polyclonal Antibody – Cat# MBS3904403

Detection of human RRP8 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from 293T cells. Antibodies: Affinity purified rabbit anti-RRP8 antibody MBS3904403 (lot MBS3904403-1) used for IP at 6 ug/mg lysate. RRP8 was also immunoprecipitated by rabbit anti-RRP8 antibody BL15381. For blotting immunoprecipitated RRP8, MBS3904402 was used at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

RRP8 Antibody – Rabbit Polyclonal

Name: RRP8 Antibody; Polyclonal; Rabbit; Human-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS3904403
Price: 250 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS3904403

Ribosomal RNA-processing protein (RRP8) is an essential component of the eNoSC (energy-dependent nucleolar silencing) complex, a complex that mediates silencing of rDNA in response to intracellular energy status and acts by recruiting histone-modifying… More details

$250 / 0.01 mg +1 FREE 8GB USB

Product Documentation

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Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

RRP8, Polyclonal Antibody

Full Product Name

Rabbit anti-RRP8 Antibody, Affinity Purified

Product Gene Name

anti-RRP8 antibody

Product Synonym Names

nucleomethylin; RRP8 methyltransferase homolog; cerebral protein 1; ribosomal RNA processing 8, methyltransferase, homolog; NML; KIAA0409; ribosomal RNA-processing protein 8; Ribosomal RNA-processing protein 8; ribosomal RNA processing 8

Clonality

Polyclonal

Host

Rabbit

Species Reactivity

Human

Form/Format

Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide

Purity/Purification

Antigen Affinity Purified

Concentration

1000ug/ml (lot specific)

Preparation and Storage

Store at 2-8 degree C
Shelf Life: 1 year from date of receipt

Molecular Weight

50,715 Da

Chromosome Location

Chromosome: 11; NC_000011.9 (6621144..6624880, complement). Location: 11p15.4

3D Structure

ModBase 3D Structure for O43159

Immunogen

Between 406 and C-terminus

Conjugation

Unconjugated

Product Categories/Family for anti-RRP8 antibody

Primary Antibodies

Applications Tested/Suitable for anti-RRP8 antibody

Immunoprecipitation (IP)

Application Notes for anti-RRP8 antibody

IP: 6ug/mg lysate

Other Notes

Small volumes of anti-RRP8 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for RRP8 purchase

MBS3904403 is an antibody targeting RRP8. Also known as Rabbit anti-RRP8 Antibody, Affinity Purified. Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for RRP8. It may not necessarily be applicable to this product.

NCBI GI #

12758125

NCBI GeneID

23378

NCBI Accession #

NP_056139.1

UniProt Primary Accession #

O43159

UniProt Secondary Accession #

Q7KZ78; Q9BVM6

UniProt Related Accession #

O43159

GenBank Nucleotide #

NM_015324.3

NCBI Official Full Name

ribosomal RNA-processing protein 8

NCBI Official Synonym Full Names

ribosomal RNA processing 8, methyltransferase, homolog (yeast)

NCBI Official Symbol

RRP8

NCBI Official Synonym Symbols

KIAA0409

UniProt Protein Name

Ribosomal RNA-processing protein 8

UniProt Synonym Protein Names

Cerebral protein 1; Nucleomethylin

Protein Family

25S rRNA

UniProt Gene Name

RRP8

UniProt Synonym Gene Names

KIAA0409; NML

UniProt Entry Name

RRP8_HUMAN

NCBI Protein Information

ribosomal RNA-processing protein 8; nucleomethylin; cerebral protein 1; RRP8 methyltransferase homolog

UniProt Comments for RRP8

RRP8: Essential component of the eNoSC (energy-dependent nucleolar silencing) complex, a complex that mediates silencing of rDNA in response to intracellular energy status and acts by recruiting histone-modifying enzymes. The eNoSC complex is able to sense the energy status of cell: upon glucose starvation, elevation of NAD(+)/NADP(+) ratio activates SIRT1, leading to histone H3 deacetylation followed by dimethylation of H3 at 'Lys- 9' (H3K9me2) by SUV39H1 and the formation of silent chromatin in the rDNA locus. In the complex, RRP8 binds to H3K9me2 and probably acts as a methyltransferase. Its substrates are however unknown. Component of the eNoSC complex, composed of SIRT1, SUV39H1 and RRP8. Belongs to the methyltransferase superfamily. RRP8 family.

Protein type: EC 2.1.1.-; RNA-binding; Nucleolus

Chromosomal Location of Human Ortholog: 11p15.4

Cellular Component: nucleoplasm; chromatin silencing complex; cytoplasm; plasma membrane; nucleolus; nucleus

Molecular Function: protein binding; S-adenosylmethionine-dependent methyltransferase activity; methylated histone residue binding

Biological Process: methylation; chromatin silencing at rDNA; cellular response to glucose starvation; transcription, DNA-dependent; negative regulation of gene expression, epigenetic; gene expression; chromatin modification; regulation of gene expression, epigenetic; regulation of transcription by glucose; rRNA processing

Research Articles on RRP8

1. These observations suggest that NML may regulate consumption of hepatic triglyceride in liver regeneration after partial hepatectomy due to storage of excess ATP.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-RRP8 antibody

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